scholarly journals Comparación teórica entre técnicas fenotípicas y genotípicas utilizadas en la identificación de Listeria monocytogenes

2021 ◽  
Vol 16 (2) ◽  
pp. 7-19
Author(s):  
Adriana Giraldo Aristizábal ◽  
Astrid Maribel Aguilera Becerra ◽  
Eliana Ximena Urbano Cáceres ◽  
Adriana María Pedraza Bernal ◽  
Claudia Patricia Jaimes Bernal
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Typing ◽  
Bacterial Typing

Listeria monocytogenes es un patógeno ubicuo intracelular, causante de la Listeriosis, la cual se considera una enfermedad transmitida por alimentos (ETA). En la actualidad existe una creciente demanda de consumidores de productos alimenticios tratados mínimamente que pueden favorecer la proliferación de este microorganismo. Es necesario contar con programas de vigilancia que incluyan métodos fiables para la detección de este patógeno en casos de brotes epidémicos. Esta revisión bibliográfica compara las ventajas y desventajas de las técnicas fenotípicas y genotípicas utilizadas en la determinación de L. monocytogenes con el fin de definir la más adecuada que permita obtener resultados confiables y en el menor tiempo posible. Se realizó una búsqueda bibliográfica en bases de datos como Pubmed, Science Direct, Proquest y Ovid, en inglés y español, utilizando los siguientes descriptores: L. monocytogenes, molecular typing, diagnosis, PCR y bacterial typing techniques. Estos se combinaron de diferentes maneras para, finalmente, recopilar setenta artículos que cumplieron con los criterios de selección propuestos. Como resultado se presentan las técnicas de diagnóstico fenotípico y genotípico que representan una opción útil para el aislamiento e identificación de este patógeno a partir de diferentes orígenes. Las técnicas revisadas permiten la diferenciación entre especies patógenas y no patógenas, así como de serotipos y genotipos con base en la implementación de procedimientos cuya fundamentación puede diferir, pero que igualmente pueden ser complementarias.

Analysis of virulence genes and molecular typing of Listeria monocytogenes isolates from human, food, and livestock from 2008 to 2016 in Iran

2021 ◽  
Vol 53 (1) ◽  
Author(s):  
Masoud Naghizadeh Heidarlo ◽  
Lida Lotfollahi ◽  
Saber Yousefi ◽  
Vahid Lohrasbi ◽  
Gholamreza Irajian ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Typing ◽  
Virulence Genes ◽  
Human Food

scholarly journals More than one variant ofListeria monocytogenesisolated from each of two human cases of invasive listeriosis

2006 ◽  
Vol 135 (5) ◽  
pp. 854-856 ◽  
Author(s):  
W. THAM ◽  
G. LOPEZ VALLADARES ◽  
S. HELMERSSON ◽  
A. ÖSTERLUND ◽  
M.-L. DANIELSSON-THAM
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Blood Cultures ◽  
Pulsed Field Gel Electrophoresis ◽  
The Other ◽  
Dna Profile ◽  
Pulsed Field ◽  
Different Strains

SUMMARYTwo variants ofListeria monocytogeneswere isolated from blood cultures from each of two patients with listeriosis. Each variant displayed a two-band difference in DNA profile from the other by pulsed-field gel electrophoresis. Although this difference in profile is insufficient to distinguish clearly between the variants, the possibility of co-infection with different strains ofL. monocytogenesneeds to be considered. We suggest that more than one colony should be selected for molecular typing to aid interpretation during investigation of the sources and routes ofListeriainfection.

P1858 Molecular typing and pathogenic potential of Listeria monocytogenes isolates from food and clinical origin

2007 ◽  
Vol 29 ◽  
pp. S532
Author(s):  
N. Papageorgiou ◽  
E. Scoulica ◽  
C. Panoulis ◽  
S. Maraki ◽  
A. Christidou ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Typing ◽  
Pathogenic Potential

scholarly journals Genetic diversity and molecular typing of Listeria monocytogenes in China

2012 ◽  
Vol 12 (1) ◽  
pp. 119 ◽  
Author(s):  
Yan Wang ◽  
Ailan Zhao ◽  
Renfa Zhu ◽  
Ruiting Lan ◽  
Dong Jin ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Listeria Monocytogenes ◽  
Molecular Typing

scholarly journals Whole genome sequencing for typing and characterisation of Listeria monocytogenes isolated in a rabbit meat processing plant

2017 ◽  
Vol 6 (3) ◽  
Author(s):  
Federica Palma ◽  
Frédérique Pasquali ◽  
Alex Lucchi ◽  
Alessandra De Cesare ◽  
Gerardo Manfreda
Keyword(s):  
Listeria Monocytogenes ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Molecular Typing ◽  
Virulence Genes ◽  
Processing Plant ◽  
Whole Genome ◽  
Meat Processing ◽  
Typing Methods ◽  
Rabbit Meat

Listeria monocytogenes is a food-borne pathogen able to survive and grow in different environments including food processing plants where it can persist for month or years. In the present study the discriminatory power of Whole Genome Sequencing (WGS)-based analysis (cgMLST) was compared to that of molecular typing methods on 34 L. monocytogenes isolates collected over one year in the same rabbit meat processing plant and belonging to three genotypes (ST14, ST121, ST224). Each genotype included isolates indistinguishable by standard molecular typing methods. The virulence potential of all isolates was assessed by Multi Virulence-Locus Sequence Typing (MVLST) and the investigation of a representative database of virulence determinant genes. The whole genome of each isolate was sequenced on a MiSeq platform. The cgMLST, MVLST, and in silico identification of virulence genes were performed using publicly available tools. Draft genomes included a number of contigs ranging from 13 to 28 and N50 ranging from 456298 to 580604. The coverage ranged from 41 to 187X. The cgMLST showed a significantly superior discriminatory power only in comparison to ribotyping, nevertheless it allows the detection of two singletons belonging to ST14 that were not observed by other molecular methods. All ST14 isolates belonged to VT107, which 7-loci concatenated sequence differs for only 4 nucleotides to VT1 (Epidemic clone III). Analysis of virulence genes showed the presence of a fulllength inlA version in all ST14 isolates and of a mutated version including a premature stop codon (PMSC) associated to attenuated virulence in all ST121 isolates.

scholarly journals Molecular Typing by Pulsed-Field Gel Electrophoresis of Spanish Animal and Human Listeria monocytogenes Isolates

2001 ◽  
Vol 67 (12) ◽  
pp. 5840-5843 ◽  
Author(s):  
A. I. Vela ◽  
J. F. Fernandez-Garayzabal ◽  
J. A. Vazquez ◽  
M. V. Latre ◽  
M. M. Blanco ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Clinical Samples ◽  
Pulsed Field ◽  
Clinical Strains ◽  
Great Genetic Diversity ◽  
Different Sources ◽  
Maize Stalks

ABSTRACT A total of 153 strains of Listeria monocytogenesisolated from different sources (72 from sheep, 12 from cattle, 18 from feedstuffs, and 51 from humans) in Spain from 1989 to 2000 were characterized by pulsed-field gel electrophoresis. The strains ofL. monocytogenes displayed 55 pulsotypes. The 84 animal, 51 human, and 18 feedstuff strains displayed 31, 29, and 7 different pulsotypes, respectively, indicating a great genetic diversity among the Spanish L. monocytogenes isolates studied. L. monocytogenes isolates from clinical samples and feedstuffs consumed by the diseased animals were analyzed in 21 flocks. In most cases, clinical strains from different animals of the same flock had identical pulsotypes, confirming the existence of a listeriosis outbreak. L. monocytogenes strains with pulsotypes identical to those of clinical strains were isolated from silage, potatoes, and maize stalks. This is the first study wherein potatoes and maize stalks are epidemiologically linked with clinical listeriosis.

scholarly journals Comparison of multilocus enzyme electrophoresis (MEE), ribotyping, restriction enzyme analysis (REA) and phage typing for typing ofListeria monocytogenes

1993 ◽  
Vol 111 (1) ◽  
pp. 71-79 ◽  
Author(s):  
B. Nørrung ◽  
P. Gerner-Smith
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Typing ◽  
Phage Typing ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Enzyme Electrophoresis ◽  
Typing Method ◽  
Multilocus Enzyme Electrophoresis ◽  
Serotype 1 ◽  
Typing Methods

SummaryThe discriminatory power of four methods for typing ofListeria monocytogeneswas compared. The four methods were multilocus enzyme electrophoresis (MEE), ribotyping, restriction enzyme analysis (REA), and a newly developed Danish phage typing system. Ninety-nine human clinical, food and slaughterhouse isolates ofListeria monocytogeneswere typed by each method. The most discriminatory single typing method was phage typing with an overall discriminatory index (DI) of 0·88 followed by REA, MEE and ribotyping with DI-values at 0·87, 0·83 and 0·79 respectively. Considering strains from each of the two predominant O-serotypes alone, serotype 1 was best discriminated by the molecular typing methods, in particular REA, which showed a DI of 0·92. The serotype 4 strains were best discriminated by phage typing (DI = 0·78). If two or more typing methods were combined, the combination of REA and MEE were found to be the most discriminatory combination. The DI values were 0·96, 0·74 and 0·90 for serotype 1, 4, and both combined, respectively. Phage typing is a rapid and inexpensive typing method but not as reproducible as the molecular typing methods. It is the most suitable method for mass screening. In situations where results are required to be highly reliable, i.e. when studying the relationships between only a few strains, a single or a combination of molecular typing methods should be used, preferable MEE and REA.

scholarly journals Selection and Identification of a Listeria monocytogenes Target Strain for Pulsed Electric Field Process Optimization

2003 ◽  
Vol 69 (4) ◽  
pp. 2223-2229 ◽  
Author(s):  
Beatrice H. Lado ◽  
Ahmed E. Yousef
Keyword(s):  
Listeria Monocytogenes ◽  
Electric Field ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Pulsed Electric Field ◽  
Pulsed Field Gel Electrophoresis ◽  
Target Strain ◽  
Acid Whey ◽  
Arbitrarily Primed Pcr ◽  
Pcr Techniques

ABSTRACT Nine Listeria monocytogenes strains were treated individually with a continuous pulsed electric field (PEF) apparatus, and their sensitivities to the treatment were compared at 25 kV/cm. When cell suspensions of these strains in 0.1% NaCl (pH 7.0) were treated at 23°C for 144 μs, inactivation ranged from 0.7 to 3.7 log10 CFU/ml. Inactivation by 72-μs PEF treatments at 37°C ranged from 0.3 to 2.5 log10 CFU/ml. L. monocytogenes OSY-8578 was substantially more resistant than other strains when cells were PEF treated in 0.1% NaCl, whereas Scott A was one of the most sensitive strains. The superiority of OSY-8578's resistance to that of Scott A was confirmed in 50% diluted acid whey (pH 4.2). Changes in sensitivity to PEF during phases of growth were minimal in OSY-8578 and substantial in Scott A. Use of L. monocytogenes OSY-8578, therefore, is recommended in studies to optimize PEF processes that target L. monocytogenes. The nine L. monocytogenes strains were genotyped with pulsed-field gel electrophoresis (PFGE) and arbitrarily primed PCR (AP-PCR) techniques. These strains were better differentiated with PFGE than with AP-PCR. The target strain (OSY-8578) was characterized by both molecular typing techniques, but resistance to PEF, in general, was not associated with a particular genotype group.

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