Genetic Characterization of Listeria monocytogenes Food Isolates and Pathogenic Potential within Serovars 1/2a and 1/2b

2004 ◽  
Vol 27 (4) ◽  
pp. 454-461 ◽  
Author(s):  
Paula Cabrita ◽  
Sónia Correia ◽  
Suzana Ferreira-Dias ◽  
Luisa Brito
Keyword(s):  
Listeria Monocytogenes ◽  
Genetic Characterization ◽  
Pathogenic Potential

scholarly journals Recurrent and Sporadic Listeria monocytogenes Contamination in Alheiras Represents Considerable Diversity, Including Virulence-Attenuated Isolates

2007 ◽  
Vol 73 (12) ◽  
pp. 3887-3895 ◽  
Author(s):  
M. T. S. Fel�cio ◽  
T. Hogg ◽  
P. Gibbs ◽  
P. Teixeira ◽  
M. Wiedmann
Keyword(s):  
Listeria Monocytogenes ◽  
Control Strategies ◽  
Tetracycline Resistance ◽  
The United States ◽  
Pulsed Field Gel Electrophoresis ◽  
Pathogenic Potential ◽  
Molecular Serotyping ◽  
Production And Distribution ◽  
Invasion Assays

ABSTRACT Microbiological characterization of alheiras, traditional smoked meat sausages produced in northern Portugal, had previously shown that more than 60% of the lots analyzed were contaminated with Listeria monocytogenes at levels higher than 100 CFU/g. In order to better understand L. monocytogenes contamination patterns in alheiras, we characterized 128 L. monocytogenes isolates from alheiras using a variety of subtyping techniques (i.e., molecular serotyping; arsenic, cadmium, and tetracycline resistance typing; and pulsed-field gel electrophoresis [PFGE]). Subtyping of isolates from products collected on two separate dates provided evidence for the persistence of specific L. monocytogenes PFGE types in the production and distribution chains of alheiras from four different processors. A subset of 21 isolates was further characterized using ribotyping and Caco-2 cell invasion assays to evaluate the pathogenic potential of L. monocytogenes present in alheiras. Caco-2 invasion assays revealed seven isolates with invasion efficiencies that were less than 20% of that of the control strain 10403S. All seven isolates had premature stop codons in inlA that represented three distinct mutations, which had previously been observed in isolates from the United States or France. Our findings indicate the need for a comprehensive approach to control L. monocytogenes in alheiras, including strategies to reduce persistence. The presence of considerable diversity in invasion phenotypes among L. monocytogenes strains present in alheiras, including the presence of subtypes likely to be virulence attenuated, may provide an opportunity to initially focus control strategies on the subtypes most likely to cause human disease.

scholarly journals Prevalence and Genetic Characterization of Listeria monocytogenes in Retail Broiler Meat in Estonia

2006 ◽  
Vol 69 (2) ◽  
pp. 436-440 ◽  
Author(s):  
KRISTI PRAAKLE-AMIN ◽  
MARJA-LIISA HÄNNINEN ◽  
HANNU KORKEALA
Keyword(s):  
Genetic Diversity ◽  
Listeria Monocytogenes ◽  
Genetic Characterization ◽  
The United States ◽  
Pulsed Field Gel Electrophoresis ◽  
Broiler Meat ◽  
Pfge Typing ◽  
Foreign Origin ◽  
Serotype 1

The prevalence and genetic diversity of Listeria monocytogenes in raw broiler legs at the retail level in Estonia were studied. A total of 240 raw broiler legs (120 from Estonia and 120 of foreign origin, which had been imported to Estonia from Denmark, Finland, Hungary, Sweden, and the United States) from 12 retail stores in the two largest cities in Estonia (Tallinn and Tartu) were investigated from January to December 2002. Of these, 70% were positive for L. monocytogenes. The prevalence of L. monocytogenes in broiler legs of Estonian origin (88%) was significantly higher than in broiler legs of foreign origin (53%) (P < 0.001). Altogether, 169 (106 Estonian and 63 imported) L. monocytogenes isolates were characterized by pulsed-field gel electrophoresis (PFGE) typing after treatment with the restriction enzyme AscI. The isolates showed a wide genetic diversity, with 35 different PFGE types obtained. Of these, 11 PFGE types came only from isolates of broiler legs of Estonian origin, 4 of Danish origin, 2 of Finnish origin, and 4 of Hungarian origin. Fourteen PFGE types came from isolates of broiler legs that originated from various countries. The strains that shared the same PFGE types from isolates of Estonian origin were recovered from broiler legs that came from different stores over the course of several months. Seventy-one L. monocytogenes isolates, including all PFGE types, were serotyped, and three serotypes (1/2a, 1/2b, and 4b) were obtained. Serotype 1/2a accounted for 96% of the isolates.

scholarly journals Comparative genetic characterization of Listeria monocytogenes isolates from human and animal listeriosis cases

Microbiology ◽  
2001 ◽  
Vol 147 (5) ◽  
pp. 1095-1104 ◽  
Author(s):  
Gregory T. Jeffers ◽  
James L. Bruce ◽  
Patrick L. McDonough ◽  
Janet Scarlett ◽  
Kathryn J. Boor ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Genetic Characterization

scholarly journals Whole-Genome Sequencing-Based Characterization of a Listeria monocytogenes Strain from an Aborted Water Buffalo in Southern Italy

2021 ◽  
Vol 9 (9) ◽  
pp. 1875
Author(s):  
Rubina Paradiso ◽  
Marita Georgia Riccardi ◽  
Bianca Cecere ◽  
Nunzia Riccone ◽  
Roberto Scialla ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Water Buffalo ◽  
Southern Italy ◽  
Antibiotic Resistance Genes ◽  
Epidemiological Studies ◽  
Whole Genome ◽  
Pathogenic Potential

Listeria monocytogenes is a Gram-positive pathogen causing life-threatening infections both in humans and animals. In livestock farms, it can persist for a long time and primarily causes uterine infections and encephalitis in farmed animals. Whole genome sequencing (WGS) is currently becoming the best method for molecular typing of this pathogen due to its high discriminatory power and efficiency of characterization. This study describes the WGS-based characterization of an L. monocytogenes strain from an aborted water buffalo fetus in southern Italy. The strain under study was classified as molecular serogroup IVb, phylogenetic lineage I, MLST sequence type 6, Clonal Complex 6, and cgMLST type CT3331, sublineage 6. Molecular analysis indicated the presence of 61 virulence genes and 4 antibiotic resistance genes. Phylogenetic analysis, including all the publicly available European L. monocytogenes serogroup IVb isolates, indicated that our strain clusterized with all the other CC6 strains and that different CCs were variably distributed within countries and isolation sources. This study contributes to the current understanding of the genetic diversity of L. monocytogenes from animal sources and highlights how the WGS strategy can provide insights into the pathogenic potential of this microorganism, acting as an important tool for epidemiological studies.

scholarly journals Prevalence, Antibiogram and Genetic Characterization of Listeria monocytogenes from Food Products in Egypt

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1381
Author(s):  
Eman E. Abdeen ◽  
Walid S. Mousa ◽  
Ola. H. Harb ◽  
Gehad A. Fath-Elbab ◽  
Mohammed Nooruzzaman ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Genetic Characterization ◽  
Food Products ◽  
Raw Milk ◽  
Food Samples ◽  
World Health ◽  
Fish Fillet ◽  
Minced Meat ◽  
The Impact

World Health Organization classified Listeria monocytogenes as a major notable foodborne pathogen associated with high mortality and hospitalization. The study reports the prevalence, antibiogram, virulence determination and genetic characterization of L. monocytogenes from different food products. A total of 250 food samples, fifty samples each from raw milk, ice cream, minced meat, fish fillet and sausage were collected from the Menoufiya governorate in Egypt. L. monocytogenes was detected in 17 (6.8%) of the tested food samples including minced meat (14%), fish fillet (8%), sausage (6%) and raw milk (6%). The antimicrobial susceptibility assay of 17 L. monocytogenes isolates against seventeen antibiotics belonging to eight antibiotics classes revealed a high susceptibility to norfloxacin (82.3%), amoxicillin-clavulanic acid (76.4%), cefotaxime (70.5%), erythromycin (64.6%), amoxicillin (64.6%), gentamicin (58.7%) and vancomycin (58.7%). While, high resistance was observed against oxytetracycline (76.4%), trimethoprim-sulfamethoxazole (76.4%), chloramphenicol (70.5%), doxycycline (64.6%), levofloxacin (41.2%) and azithromycin (41.2%). Of note, all L. monocytogenes isolates were multidrug-resistant. The multiplex PCR successfully amplified L. monocytogenes in all tested isolates. Screening of the five virulence-related genes revealed the hlyA and iap as the most prevalent genes followed by actA gene, however, the inlA and prfA genes were not detected in any of the studied isolates. The partial 16S rRNA gene sequencing of three L. monocytogenes isolates showed a high nucleotide similarity (99.1–99.8%) between the study isolates and various global clones, and phylogenetic analysis clustered these L. monocytogenes strains with other Listeria species including L. welshimeri, L. seeligeri and L. innocua. This study demonstrates the impact of L. monocytogenes as a major contaminant of various food products and suggests more attention to the awareness and hygienic measures in the food industry.

Genetic characterization of Listeria monocytogenes from ruminant listeriosis from different geographical regions in the U.S.

2018 ◽  
Vol 215 ◽  
pp. 93-97 ◽  
Author(s):  
Abbey J. Steckler ◽  
Maria X. Cardenas-Alvarez ◽  
Megan K. Townsend Ramsett ◽  
Neil Dyer ◽  
Teresa M. Bergholz
Keyword(s):  
Listeria Monocytogenes ◽  
Genetic Characterization ◽  
Geographical Regions ◽  
The U.S

scholarly journals Genetic characterization of clones of the bacterium Listeria monocytogenes causing epidemic disease.

1989 ◽  
Vol 86 (10) ◽  
pp. 3818-3822 ◽  
Author(s):  
J. C. Piffaretti ◽  
H. Kressebuch ◽  
M. Aeschbacher ◽  
J. Bille ◽  
E. Bannerman ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Genetic Characterization ◽  
Epidemic Disease

scholarly journals Genetic Characterization of a Listeria monocytogenes Serotype IVb Variant 1 Strain Isolated from Vegetal Matrix in Italy

2020 ◽  
Vol 9 (33) ◽  
Author(s):  
Marina Torresi ◽  
Massimiliano Orsini ◽  
Vicdalia Acciari ◽  
Gabriella Centorotola ◽  
Valeria Di Lollo ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Genetic Characterization ◽  
Content Type

ABSTRACT We report the chromosome and plasmid sequences of a strain of Listeria monocytogenes IVb variant 1, a recently emerging serotype, isolated in Italy from ready-to-eat vegetables.

scholarly journals Genetic Characterization of Plasmid-Associated Benzalkonium Chloride Resistance Determinants in a Listeria monocytogenes Strain from the 1998-1999 Outbreak

2010 ◽  
Vol 76 (24) ◽  
pp. 8231-8238 ◽  
Author(s):  
Driss Elhanafi ◽  
Vikrant Dutta ◽  
Sophia Kathariou
Keyword(s):  
Listeria Monocytogenes ◽  
Food Processing ◽  
Benzalkonium Chloride ◽  
Genetic Basis ◽  
Genetic Characterization ◽  
Chloride Resistance ◽  
Processing Plants ◽  
Resistant Strains ◽  
Ammonium Compounds

ABSTRACT Quaternary ammonium compounds such as benzalkonium chloride (BC) are widely used as disinfectants in both food processing and medical environments. BC-resistant strains of Listeria monocytogenes have been implicated in multistate outbreaks of listeriosis and have been frequently isolated from food processing plants. However, the genetic basis for BC resistance in L. monocytogenes remains poorly understood. In this study, we have characterized a plasmid (pLM80)-associated BC resistance cassette in L. monocytogenes H7550, a strain implicated in the 1998-1999 multistate outbreak involving contaminated hot dogs. The BC resistance cassette (bcrABC) restored resistance to BC (MIC, 40 μg/ml) in a plasmid-cured derivative of H7550. All three genes of the cassette were essential for imparting BC resistance. The transcription of H7550 BC resistance genes was increased under sublethal (10 μg/ml) BC exposure and was higher at reduced temperatures (4, 8, or 25°C) than at 37°C. The level of transcription was higher at 10 μg/ml than at 20 or 40 μg/ml. In silico analysis suggested that the BC resistance cassette was harbored by an IS1216 composite transposon along with other genes whose functions are yet to be determined. The findings from this study will further our understanding of the adaptations of this organism to disinfectants such as BC and may contribute to the elucidation of possible BC resistance dissemination in L. monocytogenes.

Sign in / Sign up

Export Citation Format

Share Document