Growth of inoculated white-rot fungi and their interactions with the bacterial community in soil contaminated with polycyclic aromatic hydrocarbons, as measured by phospholipid fatty acids

2000 ◽  
Vol 73 (1) ◽  
pp. 29-36 ◽  
Author(s):  
B.E Andersson ◽  
L Welinder ◽  
P.A Olsson ◽  
S Olsson ◽  
T Henrysson
Keyword(s):  
Fatty Acids ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Bacterial Community ◽  
Aromatic Hydrocarbons ◽  
White Rot Fungi ◽  
Phospholipid Fatty Acids ◽  
White Rot ◽  
Polycyclic Aromatic

Enhancement of bioavailability of polycyclic aromatic hydrocarbons by sequential breakdown by white-rot fungi and soil bacteria

1995 ◽  
pp. 1301-1302
Author(s):  
Rogier Meulenberg ◽  
Rob M. Hoefhagel ◽  
Carola E. M. Heeremans ◽  
Jim A. Field ◽  
Huub H. M. Rijnaarts
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Soil Bacteria ◽  
White Rot Fungi ◽  
White Rot ◽  
Polycyclic Aromatic

KEMAMPUAN DAN KEKUATAN BIOREMEDIASI AGEN HAYATI JAMUR FUNGI PELAPUK PUTIH

2018 ◽  
Vol 4 (3) ◽  
Author(s):  
Donawati Dkk
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Environmental Pollutants ◽  
White Rot Fungi ◽  
White Rot ◽  
White Rot Fungus ◽  
Huge Number ◽  
Tropical Country ◽  
The World ◽  
Polycyclic Aromatic

Indonesia is a tropical country and the richest country in the world for biodiversity, including fl ora and fauna, invertebrates, phytoplankton, bacteria, and huge number of fungus such as mushrooms, molds, mildews and smuts. White rot fungi is one of the ubiquitous creatures in Indonesia. Results indicated that white rot fungus has ability to degrade successfully some organic pollutansin the environment, such as polycyclic aromatic hydrocarbons (PAH), Polychlorinated Biphenyls (PCB’s), Trinitrotoluence (TNT), and DDT. This organism has ability to release intra and extra-cellular enzymes. There are three important rules in these degradation processes, called lignin modifi ed enzymes (LME’s) including Lignin peroxidase, Mn-dependent peroxidase and Laccase. White rot fungi contains all three enzymes and able to breakdown and mineralize several environmental pollutants into non toxic forms. This paper discuss several processes responsible for this degradation.Key words: White rot fungi, bioremediation, lignin modifi ed enzymes, persistent organic pollutants.

scholarly journals Colorimetric Assays for Biodegradation of Polycyclic Aromatic Hydrocarbons by Fungal Laccases

2002 ◽  
Vol 7 (6) ◽  
pp. 547-553 ◽  
Author(s):  
Miguel Alcalde ◽  
Thomas Bulter ◽  
Frances H. Arnold
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Solid Phase ◽  
White Rot Fungi ◽  
Water Soluble ◽  
White Rot ◽  
Fungal Laccases ◽  
Polycyclic Aromatic ◽  
Colorimetric Assays ◽  
Solid Phase Assay

Polycyclic aromatic hydrocarbons (PAHs) are highly toxic organic pollutants widely distributed in terrestrial and aquatic environments. In the present work, 2 colorimetric assays for laccase-catalyzed degradation of PAHs were developed based on studies of the oxidation of 12 aromatic hydrocarbons by fungal laccases from Trametes versicolor and Myceliophthora thermophila. Using a sodium borohydride water-soluble solution, the authors could reduce the single product of laccase-catalyzed anthracene biooxidation into the orange-colored 9,10-anthrahydroquinone, which is quantifiable spectrophotometrically. An assay using polymeric dye (Poly R-478) as a surrogate substrate for lignin degradation by laccase in the presence of mediator is also presented. The decolorization of Poly R-478 was correlated to the oxidation of PAHs mediated by laccases. This demonstrates that a ligninolytic indicator such as Poly R-478 can be used to screen for PAH-degrading laccases; it will also be useful in screening mutant libraries in directed evolution experiments. Poly R-478 is stable and readily soluble. It has a high extinction coefficient and low toxicity toward white rot fungi, yeast, and bacteria, which allow its application in a solid-phase assay format.

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