Immunofluorescent Study of Human MII Oocytes Failed to Fertilize after IVF and ICSI

2010 ◽  
Vol 21 (1) ◽  
pp. 9-15
Author(s):  
Hai-ning LUO ◽  
Gui-jin ZHU ◽  
Juan HU ◽  
Yan-lin WANG ◽  
Yu-lan WEI
Keyword(s):  
Immunofluorescent Study

Etude par immunofluorescence de l'apparition d'antigènes neuro-spécifiques d'adulte chez l'embryon de Poulet

Development ◽  
1977 ◽  
Vol 39 (1) ◽  
pp. 1-7
Author(s):  
N. Touzet ◽  
R. Jeanmaire-Zylberberg ◽  
M. Chaminade
Keyword(s):  
Chick Embryo ◽  
Outer Layer ◽  
Immunofluorescent Study ◽  
Specific Antigens ◽  
Fluorescent Cells ◽  
Marked Fluorescence

Immunofluorescent study of the distribution of adult neuro-specific antigens in the chick embryo The adult neuro-specific antigens have been localized by immunofluorescence techniques in diencephalon and mesencephalon of chick embryo. This study has been made using fresh or fixed tissues from embryos 72, 48 or 36 h old. At 72 h of incubation the wall of diencephalon shows marked fluorescence; at 48 h of incubation the fluorescent cells are localized in an outer layer and an inner one. In the 48 h-old embryo the reaction is more distinct and intensive in fresh tissues than in fixed tissues. At 36 h of incubation no fluorescence has been detected either in fresh tissues or in fixed tissues.

Identification Of The AT III Synthesizing Hepatocytes By Immunofluorescent Technique

1981 ◽  
Author(s):  
M Watada ◽  
M Nakagawa ◽  
T Kitani ◽  
Y Okajima ◽  
Y Maeda ◽  
...  
Keyword(s):  
Rat Liver ◽  
Gamma Globulin ◽  
Regulatory Mechanism ◽  
Antithrombin Iii ◽  
Immunofluorescent Staining ◽  
Immunofluorescent Study ◽  
At Iii ◽  
Immunofluorescent Technique ◽  
Reported Data ◽  
Indirect Immunofluorescent

Antithrombin III (AT III), heparin cofactor has been known to be one of the important protease inhibitors on the regulatory mechanism of coagulation and fibrinolysis, but the site of synthesis of plasma AT HI has not yet been conclusively established, although some observations suggested that the major site of AT III production may be the liver and AT III levels in plasma is known to be decreased on the hepatic disorders. This paper reports the deposition of the AT III specific fluorescence in the hepatocytes of rat liver by immunofluorescent examination. Plasma AT III was purified by heparin sepharose affinity chromatography and gel filtrations. Rabbit was immunized with this purified AT III and Freund’s complete adjuvant and rabbit anti rat AT III serum was obtained. This antiserum was comfirmed to be monospecific to rat AT HI by means of immunoelectrophoresis. Freshly isolated rat liver was prepared as specimen and fixed in acid alcohol and processed to dehydration and embedding into paraffin in conventional way. Indirect immunofluorescent technique with FITC labeled anti rabbit gamma-globulin were utilized for immunofluorescent staining and histological observation was performed under Nikon FL fluorescent microscope. The localization of fluorescence was observed in the hepatocytes which scattered in the acinus structures. The fluorescence in the cytoplasma of these hepatocytes was homogeneously stained. As far as the fluorescence is concerned, the synthesis of AT III may be performed not synchronizing among hepatocytes. This immunofluorescent study supports the reported data of hepatic perfusion.

DIRECT IMMUNOFLUORESCENT STUDY OF THE SKIN ON OCCURRENCE OF COMPLEMENT IN PEMPHIGUS

1972 ◽  
Vol 87 (5) ◽  
pp. 466-474 ◽  
Author(s):  
TH. JOOST ◽  
R. H. CORMANE ◽  
K. W. PONDMAN
Keyword(s):  
Immunofluorescent Study

Immunofluorescent Study on Middle Ear Mucosa

1980 ◽  
Vol 89 (3_suppl) ◽  
pp. 333-338 ◽  
Author(s):  
Goro Mogi ◽  
Shoichi Maeda ◽  
Noritake Watanabe
Keyword(s):  
Middle Ear ◽  
Guinea Pigs ◽  
Secretory Component ◽  
Direct Immunofluorescence ◽  
Secretory Proteins ◽  
Tympanic Cavity ◽  
Defense System ◽  
Middle Ear Mucosa ◽  
Local Synthesis ◽  
Immunofluorescent Study

To clarify the developmental course of the immunological defense system in the middle ear, immunoglobulin-forming cells of different classes and secretory proteins, such as secretory component (SC) and lactoferrin (Lf), were investigated in the middle ear mucosa of 20 developing and 5 normal adult guinea pigs by use of direct immunofluorescence technique. Changes in the middle ear mucosa were also observed after antigenic challenges directly to the tympanic cavity of 6 developing and 39 adult guinea pigs. IgA- and IgM-forming cells began to appear in the tubal mucosa at the seventh postnatal day, while it was scarcely possible to find IgG1- and IgG2-forming cells in developing guinea pigs. Immunoglobulin-forming cells of all classes increased in the middle ear mucosa after the antigenic stimuli. The injection of antigens to the tympanic cavity of developing animals induced the most striking accumulation of immunoglobulin-forming cells in the middle ear mucosa. Results of this study showed that local synthesis of IgA, as well as other classes, is latent in the middle ear, that the middle ear of immature animals is vulnerable to antigenic stimuli, and that the middle ear of developing animals possesses potential immune responsiveness.

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