Linoleic Acid Activates GPR40/FFA1 and Phospholipase C to Increase [Ca2+]i Release and Insulin Secretion in Islet Beta-Cells

2012 ◽  
Vol 27 (1) ◽  
pp. 18-23 ◽  
Author(s):  
Yi-jun Zhou ◽  
Yu-ling Song ◽  
Hui Zhou ◽  
Yan Li
Keyword(s):  
Insulin Secretion ◽  
Linoleic Acid ◽  
Phospholipase C ◽  
Beta Cells ◽  
Islet Beta Cells

scholarly journals Overexpression of beta 2-microglobulin in transgenic mouse islet beta cells results in defective insulin secretion.

1991 ◽  
Vol 88 (6) ◽  
pp. 2070-2074 ◽  
Author(s):  
J. Allison ◽  
L. Malcolm ◽  
J. Culvenor ◽  
R. K. Bartholomeusz ◽  
K. Holmberg ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Transgenic Mouse ◽  
Beta Cells ◽  
Mouse Islet ◽  
Islet Beta Cells ◽  
Beta 2 ◽  
Beta 2 Microglobulin

scholarly journals Engineering of Glycerol-stimulated Insulin Secretion in Islet Beta Cells

1997 ◽  
Vol 272 (30) ◽  
pp. 18621-18627 ◽  
Author(s):  
Richard J. Noel ◽  
Peter A. Antinozzi ◽  
J. Denis McGarry ◽  
Christopher B. Newgard
Keyword(s):  
Insulin Secretion ◽  
Beta Cells ◽  
Islet Beta Cells

Niacin receptor GPR109A inhibits insulin secretion and is down-regulated in type 2 diabetic islet beta-cells

2016 ◽  
Vol 237 ◽  
pp. 98-108 ◽  
Author(s):  
Na Wang ◽  
De-yu Guo ◽  
Xiong Tian ◽  
Hao-peng Lin ◽  
Yun-pan Li ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Beta Cells ◽  
Islet Beta Cells ◽  
Type 2 Diabetic

scholarly journals Purification of age-distinct insulin secretory granules through antigen restriction using the CLIP-tag

2020 ◽  
Author(s):  
Martin Neukam ◽  
Katharina Ganß ◽  
Jovana Vasiljević ◽  
Johannes Broichhagen ◽  
Kai Johnsson ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Insulin Secretion ◽  
Beta Cells ◽  
Solid Phase ◽  
Secretory Granules ◽  
Islet Beta Cells ◽  
Temporal Variables ◽  
Pancreatic Islet Beta Cells ◽  
Insulin Granules ◽  
Insulin Granule

AbstractPancreatic islet beta cells employ secretory granules for the storage and glucose-stimulated release of the hormone insulin. The competence of an insulin granule for exocytosis depends on spatial and temporal variables such as its proximity to the plasma membrane as well as its age, with newly-generated granules being preferentially released. The molecular underpinnings for the control of these variables remain largely unknown and their uncovering is of high relevance for the study of diabetes, which results from deficient insulin secretion. However, we still lack a comprehensive view about the molecular composition of the insulin granules and how this may change over their lifetime. Here we report a strategy for the background-free purification of insulin secretory granules of distinct age from insulinoma INS-1 cells. We show that utilization of an immuno-based affinity approach for pulse-chase labeled insulin secretory granules, produces a highly enriched granular fraction. Our approach precludes typical contaminants from the solid phase and may be designed to purify secretory granules of a distinct age.

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