A steady state fluorescence study of nonamphiphilic N,N′-bis(2,6-dimethylphenyl)-3,4,9,10-perylenetetracarboxylic diimide in supramolecular Langmuir-Blodgett assemblies

1998 ◽  
Vol 114 (2) ◽  
pp. 145-150 ◽  
Author(s):  
A.K. Dutta
Keyword(s):  
Steady State ◽  
Fluorescence Study ◽  
Langmuir Blodgett ◽  
Steady State Fluorescence

Organization of Cationic Porphyrins in Mixed Langmuir−Blodgett Films. An Absorption and Steady-State Fluorescence Study

Langmuir ◽  
2002 ◽  
Vol 18 (15) ◽  
pp. 5772-5781 ◽  
Author(s):  
Maria Isabel Viseu ◽  
Amélia M. Gonçalves da Silva ◽  
Patrícia Antunes ◽  
Sílvia M. B. Costa
Keyword(s):  
Steady State ◽  
Fluorescence Study ◽  
Cationic Porphyrins ◽  
Langmuir Blodgett ◽  
Steady State Fluorescence ◽  
Langmuir Blodgett Films

scholarly journals Effect of PAF on polyrnorphonuclear leucocyte plasma membrane polarity: a fluorescence study

1993 ◽  
Vol 2 (2) ◽  
pp. 149-151 ◽  
Author(s):  
A. Kantar ◽  
P. L. Giorgi ◽  
R. Fiorini
Keyword(s):  
Plasma Membrane ◽  
Steady State ◽  
Polymorphonuclear Leukocytes ◽  
Fluorescence Emission ◽  
Emission Spectra ◽  
Blue Shift ◽  
Fluorescence Study ◽  
Polar Solvents ◽  
Fluorescence Emission Spectra ◽  
Steady State Fluorescence

The effect of PAF on the plasma membrane polarity of polymorphonuclear leukocytes (PMNs) was investigated by measuring the steady-state fluorescence emission spectra of 2-dimethylamino(6-1auroyl) naphthalene (Laurdan), which is known to be incorporated at the hydrophobic-hydrophilic interface of the bilayer, displaying spectral sensitivity to the polarity of its surrounding. Laurdan shows a marked steady-state emission blue-shift in non-polar solvents, with respect to polar solvents. Our results demonstrate that PAF (10−7M) induces a blue shift of the fluorescence emission spectra of Laurdan. These changes are blocked in the presence of the PAF antagonist, L-659,989. Our data indicate that the interaction between PAF and PMNs is accompanied by a decrease in polarity in the hydrophobic-hydrophilic interface of the plasma membrane.

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