Overexpression of DsbC and DsbG markedly improves soluble and functional expression of single-chain Fv antibodies in Escherichia coli

2002 ◽  
Vol 26 (2) ◽  
pp. 218-228 ◽  
Author(s):  
Zhong Zhang ◽  
Zhi-Hua Li ◽  
Fei Wang ◽  
Min Fang ◽  
Chang-Cheng Yin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Functional Expression ◽  
Single Chain ◽  
Single Chain Fv

Functional expression of single-chain variable fragment antibody against c-Met in the cytoplasm of Escherichia coli

2006 ◽  
Vol 47 (1) ◽  
pp. 203-209 ◽  
Author(s):  
Mi-Ae Heo ◽  
Su-Hyun Kim ◽  
So-Yeon Kim ◽  
Yu-Jin Kim ◽  
Junho Chung ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Functional Expression ◽  
Single Chain Variable Fragment ◽  
Single Chain

scholarly journals Specific Secretion of Active Single-Chain Fv Antibodies into the Supernatants of Escherichia coliCultures by Use of the Hemolysin System

2000 ◽  
Vol 66 (11) ◽  
pp. 5024-5029 ◽  
Author(s):  
Luis A. Fernández ◽  
Isabel Sola ◽  
Luis Enjuanes ◽  
Víctor de Lorenzo
Keyword(s):  
Escherichia Coli ◽  
Transport System ◽  
Culture Media ◽  
Single Chain ◽  
Extracellular Medium ◽  
Simple Method ◽  
E Coli ◽  
Single Chain Fv ◽  
Bacterial Cytoplasm ◽  
Culture Supernatants

ABSTRACT A simple method for the nontoxic, specific, and efficient secretion of active single-chain Fv antibodies (scFvs) into the supernatants ofEscherichia coli cultures is reported. The method is based on the well-characterized hemolysin transport system (Hly) of E. coli that specifically secretes the target protein from the bacterial cytoplasm into the extracellular medium without a periplasmic intermediate. The culture media that accumulate these Hly-secreted scFv's can be used in a variety of immunoassays without purification. In addition, these culture supernatants are stable over long periods of time and can be handled basically as immune sera.

scholarly journals Neutralizationof Enteric Coronaviruses with Escherichia coli CellsExpressing Single-Chain Fv-AutotransporterFusions

2003 ◽  
Vol 77 (24) ◽  
pp. 13396-13398 ◽  
Author(s):  
Esteban Veiga ◽  
Víctor de Lorenzo ◽  
Luis Angel Fernández
Keyword(s):  
Escherichia Coli ◽  
Infectious Agent ◽  
Target Cells ◽  
Single Chain ◽  
E Coli ◽  
Single Chain Antibodies ◽  
Single Chain Fv ◽  
Transmissible Gastroenteritis ◽  
Iga Protease

ABSTRACT We report here that fusions of single-chain antibodies (scFvs) to the autotransporter β domain of the IgA protease of Neisseria gonorrhoeae are instrumental in locating virus-neutralizing activity on the cell surface of Escherichia coli. E. coli cells displaying scFvs against the transmissible gastroenteritis coronavirus on their surface blocked in vivo the access of the infectious agent to cultured epithelial cells. This result raises prospects for antiviral strategies aimed at hindering the entry into target cells by bacteria that naturally colonize the same intestinal niches.

Generation of single-chain Fv antibody fragments against Mu-2-related death-inducing gene in Escherichia coli

2019 ◽  
Vol 46 (4) ◽  
pp. 4027-4037
Author(s):  
Dimuthu Dhammika Wickramanayake ◽  
Jun-Ha Choi ◽  
Juhyun Shin ◽  
Jae-Wook Oh
Keyword(s):  
Escherichia Coli ◽  
Antibody Fragments ◽  
Single Chain ◽  
Single Chain Fv ◽  
Fv Antibody ◽  
Single Chain Fv Antibody

Expression and purification of an anti-clenbuterol single chain Fv antibody in Escherichia coli

2010 ◽  
Vol 72 (1) ◽  
pp. 26-31 ◽  
Author(s):  
Hong Wang ◽  
Xixia Liu ◽  
Yongsheng He ◽  
Jiexian Dong ◽  
Yuanming Sun ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Single Chain ◽  
Expression And Purification ◽  
Single Chain Fv ◽  
Fv Antibody ◽  
Single Chain Fv Antibody
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