Specific Secretion of Active Single-Chain Fv Antibodies into the Supernatants of Escherichia coliCultures by Use of the Hemolysin System
2000 ◽
Vol 66
(11)
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pp. 5024-5029
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Keyword(s):
E Coli
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ABSTRACT A simple method for the nontoxic, specific, and efficient secretion of active single-chain Fv antibodies (scFvs) into the supernatants ofEscherichia coli cultures is reported. The method is based on the well-characterized hemolysin transport system (Hly) of E. coli that specifically secretes the target protein from the bacterial cytoplasm into the extracellular medium without a periplasmic intermediate. The culture media that accumulate these Hly-secreted scFv's can be used in a variety of immunoassays without purification. In addition, these culture supernatants are stable over long periods of time and can be handled basically as immune sera.
1998 ◽
Vol 64
(12)
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pp. 4862-4869
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2003 ◽
Vol 102
(2)
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pp. 177-189
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Keyword(s):
2011 ◽
Vol 64
(8)
◽
pp. 1736-1742
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1988 ◽
Vol 85
(16)
◽
pp. 5879-5883
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Keyword(s):