A growing family of cytochrome b5-domain fusion proteins

1999 ◽  
Vol 4 (1) ◽  
pp. 2-4 ◽  
Author(s):  
Johnathan A. Napier ◽  
Olga Sayanova ◽  
Petra Sperling ◽  
Ernst Heinz
Keyword(s):  
Fusion Proteins ◽  
Cytochrome B5 ◽  
Domain Fusion

scholarly journals The C-terminus of cytochrome b5 confers endoplasmic reticulum specificity by preventing spontaneous insertion into membranes

2007 ◽  
Vol 401 (3) ◽  
pp. 701-709 ◽  
Author(s):  
Matthew P. A. Henderson ◽  
Yeen Ting Hwang ◽  
John M. Dyer ◽  
Robert T. Mullen ◽  
David W. Andrews
Keyword(s):  
Endoplasmic Reticulum ◽  
Subcellular Localization ◽  
Lipid Bilayers ◽  
Fusion Proteins ◽  
Molecular Mechanisms ◽  
Cytochrome B5 ◽  
Terminal Sequence ◽  
C Terminus

The molecular mechanisms that determine the correct subcellular localization of proteins targeted to membranes by tail-anchor sequences are poorly defined. Previously, we showed that two isoforms of the tung oil tree [Vernicia (Aleurites) fordii] tail-anchored Cb5 (cytochrome b5) target specifically to ER (endoplasmic reticulum) membranes both in vivo and in vitro [Hwang, Pelitire, Henderson, Andrews, Dyer and Mullen (2004) Plant Cell 16, 3002–3019]. In the present study, we examine the targeting of various tung Cb5 fusion proteins and truncation mutants to purified intracellular membranes in vitro in order to assess the importance of the charged CTS (C-terminal sequence) in targeting to specific membranes. Removal of the CTS from tung Cb5 proteins resulted in efficient binding to both ER and mitochondria. Results from organelle competition, liposome-binding and membrane proteolysis experiments demonstrated that removal of the CTS results in spontaneous insertion of tung Cb5 proteins into lipid bilayers. Our results indicate that the CTSs from plant Cb5 proteins provide ER specificity by preventing spontaneous insertion into incorrect subcellular membranes.

scholarly journals Inhibition of Tat-mediated transactivation and HIV replication with Tat mutant and repressor domain fusion proteins

Gene Therapy ◽  
1998 ◽  
Vol 5 (7) ◽  
pp. 946-954 ◽  
Author(s):  
C Fraisier ◽  
DA Abraham ◽  
M van Oijen ◽  
V Cunliffe ◽  
A Irvine ◽  
...  
Keyword(s):  
Fusion Proteins ◽  
Hiv Replication ◽  
Domain Fusion

Matrix-Assisted Refolding of Single-Chain Fv– Cellulose Binding Domain Fusion Proteins

1999 ◽  
Vol 17 (2) ◽  
pp. 249-259 ◽  
Author(s):  
Yevgeny Berdichevsky ◽  
Raphael Lamed ◽  
Dan Frenkel ◽  
Uri Gophna ◽  
Edward A. Bayer ◽  
...  
Keyword(s):  
Fusion Proteins ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Single Chain ◽  
Domain Fusion ◽  
Single Chain Fv ◽  
Cellulose Binding

Enhanced antiangiogenic therapy with antibody-collagen XVIII NC1 domain fusion proteins engineered to exploit matrix remodeling events

2006 ◽  
Vol 119 (2) ◽  
pp. 455-462 ◽  
Author(s):  
Víctor J. Sánchez-Arévalo Lobo ◽  
Ángel M. Cuesta ◽  
Laura Sanz ◽  
Marta Compte ◽  
Pascal García ◽  
...  
Keyword(s):  
Fusion Proteins ◽  
Antiangiogenic Therapy ◽  
Matrix Remodeling ◽  
Domain Fusion ◽  
Collagen Xviii ◽  
Nc1 Domain
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