O6 Reanalysis of day 5 embryos following preimplantation genetic screening (PGS) for chromosomal abnormalities by fluorescence in situ hybridization on a single blastomere among different indication groups

2010 ◽  
Vol 20 ◽  
pp. S17
Author(s):  
D. Christopikou ◽  
E. Tsorva ◽  
A. Garantzioti ◽  
M. Argyrou ◽  
M. Moschopoulou ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Genetic Screening ◽  
Chromosomal Abnormalities ◽  
Preimplantation Genetic Screening ◽  
Single Blastomere

scholarly journals Application of the FISH Technique to Visualize Sex Chromosomes in Domestic Cat Spermatozoa

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2106
Author(s):  
Barbara Kij-Mitka ◽  
Halina Cernohorska ◽  
Svatava Kubickova ◽  
Sylwia Prochowska ◽  
Wojciech Niżański ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Sex Chromosomes ◽  
Chromosomal Abnormalities ◽  
Molecular Cytogenetics ◽  
Molecular Probes ◽  
Domestic Cat ◽  
Fish Technique ◽  
Y Chromosomes

Fluorescence in situ hybridization is a molecular cytogenetics technique that enables the visualization of chromosomes in cells via fluorescently labeled molecular probes specific to selected chromosomes. Despite difficulties in carrying out the FISH technique on sperm, related to the need for proper nuclear chromatin decondensation, this technique has already been used to visualize chromosomes in human, mouse, cattle, swine, horse, and dog spermatozoa. Until now, FISH has not been performed on domestic cat sperm; therefore, the aim of this study was to visualize sex chromosomes in domestic cat sperm. The results showed the presence of X and Y chromosomes in feline spermatozoa. The procedure used for sperm decondensation and fluorescence in situ hybridization was adequate to visualize chromosomes in domestic cat spermatozoa and, in the future, it may be used to determine the degree of chromosomal abnormalities in these gametes.

Characterization of quantitative chromosomal abnormalities in renal cell carcinomas by interphase four-color fluorescence in situ hybridization

2005 ◽  
Vol 158 (2) ◽  
pp. 110-118 ◽  
Author(s):  
Aline Ossard Receveur ◽  
Jérôme Couturier ◽  
Vincent Molinié ◽  
Annick Vieillefond ◽  
François Desangles ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Renal Cell ◽  
Chromosomal Abnormalities ◽  
Renal Cell Carcinomas
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