Abstract #790986: Single Nucleotide Polymorphisms at + 276 G > T and at + 45 T > G of the Adiponectin Gene and Plasma Adiponectin Level in Myanmar Type 2 Diabetic Patients

Objective: to study the effect of MATE 1, MATE 2 and OCT1 genetic variants on metformin action in recently diagnosed Egyptian Type-2 diabetic patients. Patients & Methods: One hundred type-2 DM patients and forty healthy control were included in the study. All patients were recently diagnosed receiving no treatment before participation in the study. Three single nucleotide polymorphisms (SNPs) were Genotyped using real time PCR, Sequence Detection System: MATE1 (rs2252281), OCT1 coding variants (rs12208357) (SLC22A1) and MATE2 (rs12943590). Results: there is a significant differences between control and patients regarding MATE2 (p<0.05), OCT1 (P<0.005) distribution; in which GG (54%), CC (62%) is the most prevalent among studied patients respectively. MATE1 SNP; Patients with CC alleles and TT allele had better HBA1C (8.577±.2924), (8.7±.25) compared to CT allele patients (9.584±.3023) (P= .04) (P=.019) respectively. OCT1 SNP; CG allele patients showed better RBS (251±9.565) compared to CC allele (294.42±8.476) (p=0.004). Logistic regression test showed that RBS (p=.00001), ALT (p=.0001) and TLC (p=.025) are independent factors affecting blood glucose. Conclusion: MATE1 and OCT1 SNPs may have a potential role in metformin efficacy.

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Associations between two common single nucleotide polymorphisms (rs2241766 and rs1501299) of ADIPOQ gene and coronary artery disease in type 2 diabetic patients: a systematic review and meta-analysis

Oncotarget ◽

10.18632/oncotarget.18317 ◽

2017 ◽

Vol 8 (31) ◽

pp. 51994-52005 ◽

Cited By ~ 2

Author(s):

Na Zhao ◽

Ningxia Li ◽

Shengjun Zhang ◽

Qiang Ma ◽

Cong Ma ◽

...

Keyword(s):

Systematic Review ◽

Meta Analysis ◽

Diabetic Patients ◽

Nucleotide Polymorphisms ◽

Type 2 Diabetic Patients ◽

Single Nucleotide ◽

Adipoq Gene ◽

Artery Disease ◽

Type 2 Diabetic

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The +276 G/T Single Nucleotide Polymorphism of the Adiponectin Gene Is Associated With Coronary Artery Disease in Type 2 Diabetic Patients

Diabetes Care ◽

10.2337/diacare.27.8.2015 ◽

2004 ◽

Vol 27 (8) ◽

pp. 2015-2020 ◽

Cited By ~ 100

Author(s):

S. Bacci ◽

C. Menzaghi ◽

T. Ercolino ◽

X. Ma ◽

A. Rauseo ◽

...

Keyword(s):

Coronary Artery Disease ◽

Single Nucleotide Polymorphism ◽

Diabetic Patients ◽

Adiponectin Gene ◽

Nucleotide Polymorphism ◽

Type 2 Diabetic Patients ◽

Single Nucleotide ◽

Artery Disease ◽

Type 2 Diabetic

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Association of genetic variants in the adiponectin gene with adiponectin level and hypertension in Hong Kong Chinese

Acta Endocrinologica ◽

10.1530/eje-10-0251 ◽

2010 ◽

Vol 163 (2) ◽

pp. 251-257 ◽

Cited By ~ 50

Author(s):

Kwok Leung Ong ◽

Mingfang Li ◽

Annette W K Tso ◽

Aimin Xu ◽

Stacey S Cherny ◽

...

Keyword(s):

Hong Kong ◽

Genetic Variants ◽

Adiponectin Level ◽

Sandwich Elisa ◽

Resistance Index ◽

Nucleotide Polymorphisms ◽

Adiponectin Gene ◽

Plasma Adiponectin ◽

Plasma Adiponectin Level ◽

Significant Independent Factor

ObjectiveLow plasma adiponectin level can predict the development of hypertension after 5 years in our population. We therefore investigated whether single-nucleotide polymorphisms (SNPs) in the adiponectin gene influenced plasma adiponectin level and whether they were associated with hypertension.Design and methodsWe genotyped 14 tagging SNPs in 1616 subjects with persistent normotensive or hypertensive status during a 6.4-year follow-up period in the Hong Kong Cardiovascular Risk Factor Prevalence Study-2 (CRISPS-2). Plasma adiponectin level was measured in 1385 subjects using in-house sandwich ELISA.ResultsThe minor G allele of the SNP rs266729 was significantly associated with higher odds of hypertension (odds ratio (95% confidence interval)=1.49 (1.13–1.95), P=0.0044) after adjusting for covariates. In stepwise multiple logistic regression, this SNP (P=0.006) was a significant independent factor of hypertension, together with age (P<0.001), body mass index (P<0.001), triglycerides (P=0.021), and insulin resistance index (P<0.001). Among the 14 SNPs, rs266729 (β=−0.067, P=0.0037), −10677C>T (β=0.069, P=0.0027), rs182052 (β=−0.097, P<0.0001), and rs12495941 (β=0.103, P<0.0001) were significantly associated with adiponectin level after adjusting for covariates. No significant sex interaction was found for the associations of SNPs with hypertension and adiponectin level. Similar results were obtained in haplotype analysis.ConclusionIn our population, genetic variants in the adiponectin gene influenced plasma adiponectin levels, and one of them was associated with hypertension. This study has provided further evidence for a role of adiponectin in the development of hypertension.

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Hypoadiponectinemia Is Associated with Impaired Endothelium-Dependent Vasodilation

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jc.2003-031012 ◽

2004 ◽

Vol 89 (2) ◽

pp. 765-769 ◽

Cited By ~ 244

Author(s):

K. C. B. Tan ◽

A. Xu ◽

W. S. Chow ◽

M. C. W. Lam ◽

V. H. G. Ai ◽

...

Keyword(s):

Endothelial Cells ◽

Brachial Artery ◽

Adiponectin Level ◽

Univariate Analysis ◽

Diabetic Patients ◽

Type 2 Diabetic Patients ◽

Brachial Artery Diameter ◽

Plasma Adiponectin ◽

Plasma Adiponectin Level

Adiponectin may have an antiatherogenic effect by reducing endothelial activation. We hypothesized that plasma adiponectin levels were correlated with endothelial function. Plasma adiponectin level was determined by an in-house RIA assay using a rabbit polyclonal antibody in 73 type 2 diabetic patients and 73 controls. Endothelium-dependent and independent vasodilation of the brachial artery was measured by high-resolution vascular ultrasound. Plasma adiponectin level was lower in diabetic patients than in controls (4.73 ± 1.96 vs. 7.69 ± 2.80 μg/ml, respectively; P < 0.001), and they also had impaired endothelium-dependent (5.6 ± 3.6 vs. 8.6 ± 4.5%, respectively; P < 0.001) and -independent vasodilation (13.3 ± 4.9 vs. 16.5 ± 5.6%, respectively; P < 0.001). Plasma adiponectin correlated with endothelium-dependent vasodilation in controls (P = 0.02) and diabetic patients (P = 0.04). On general linear-model univariate analysis, brachial artery diameter, the presence of diabetes, plasma adiponectin, and high-density lipoprotein were significant independent determinants of endothelium-dependent vasodilation. In vitro experiments showed that endothelial cells expressed adiponectin receptors, and adiponectin increased nitric oxide production in human aortic endothelial cells. In conclusion, low plasma adiponectin level is associated with impaired endothelium-dependent vasodilation, and the association is independent of diabetes mellitus. Adiponectin may act as a link between adipose tissue and the vasculature.

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