OC.05.4: MUCOSAL MEDIATORS FROM PATIENTS WITH IRRITABLE BOWEL SYNDROME STIMULATE ENTERIC CHOLINERGIC MOTOR NEURONS IN VITRO VIA PURINERGIC, PROSTAGLANDIN, AND TRPV1 PATHWAYS

2011 ◽  
Vol 43 ◽  
pp. S129
Author(s):  
C. Cremon ◽  
B. Balestra ◽  
G. Dothel ◽  
G. Carini ◽  
L. Zecchi ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Motor Neurons ◽  
Irritable Bowel

scholarly journals The Impact of Low-Level Iron Supplements on the Faecal Microbiota of Irritable Bowel Syndrome and Healthy Donors Using In Vitro Batch Cultures

Nutrients ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3819
Author(s):  
Carlos Poveda ◽  
Dora I. A. Pereira ◽  
Marie C. Lewis ◽  
Gemma E. Walton
Keyword(s):  
Irritable Bowel Syndrome ◽  
Gut Microbiota ◽  
Iron Supplementation ◽  
Ferrous Sulphate ◽  
Fluorescence In Situ Hybridisation ◽  
Batch Cultures ◽  
Low Level ◽  
Irritable Bowel ◽  
The Impact

Ferrous iron supplementation has been reported to adversely alter the gut microbiota in infants. To date, the impact of iron on the adult microbiota is limited, particularly at low supplementary concentrations. The aim of this research was to explore the impact of low-level iron supplementation on the gut microbiota of healthy and Irritable Bowel Syndrome (IBS) volunteers. Anaerobic, pH-controlled in vitro batch cultures were inoculated with faeces from healthy or IBS donors along with iron (ferrous sulphate, nanoparticulate iron and pea ferritin (50 μmol−1 iron)). The microbiota were explored by fluorescence in situ hybridisation coupled with flow cytometry. Furthermore, metabolite production was assessed by gas chromatography. IBS volunteers had different starting microbial profiles to healthy controls. The sources of iron did not negatively impact the microbial population, with results of pea ferritin supplementation being similar to nanoparticulate iron, whilst ferrous sulphate led to enhanced Bacteroides spp. The metabolite data suggested no shift to potentially negative proteolysis. The results indicate that low doses of iron from the three sources were not detrimental to the gut microbiota. This is the first time that pea ferritin fermentation has been tested and indicates that low dose supplementation of iron is unlikely to be detrimental to the gut microbiota.

Excitatory effect of colonic mast cell mediators of irritable bowel syndrome patients on enteric cholinergic motor neurons

2006 ◽  
Vol 38 ◽  
pp. S33 ◽  
Author(s):  
C. Cremon ◽  
G. Barbara ◽  
R. Vicini ◽  
E. Cervio ◽  
B. Balestra ◽  
...  
Keyword(s):  
Mast Cell ◽  
Irritable Bowel Syndrome ◽  
Motor Neurons ◽  
Excitatory Effect ◽  
Irritable Bowel

scholarly journals Increased Vδ1γδT cells Predominantly Contributed to IL-17 Production in the Development of Adult Human Post-infectious Irritable Bowel Syndrome

2020 ◽  
Author(s):  
liwei dong ◽  
xiaoning sun ◽  
zhichao ma ◽  
jiao fu ◽  
fujin liu ◽  
...  
Keyword(s):  
T Cells ◽  
Irritable Bowel Syndrome ◽  
Peripheral Blood ◽  
Γδ T Cells ◽  
Γδt Cells ◽  
Peripheral T Cells ◽  
Confocal Fluorescence ◽  
Irritable Bowel ◽  
Post Infectious

Abstract Background: γδT cells play an important role in the mucosa inflammation and immunity-associated disorders. Our previous study reported that γδT cells producing IL-17 were involved in the pathogenesis of post-infectious irritable bowel syndrome (PI-IBS). However, their subset characteristic profile in this kind of disease remains unclear. Thus the current study’s aim is to investigate the functionally predominant subset and its role in PI-IBS. Methods: The total T cells were collected from the peripheral blood of patients with PI-IBS. The peripheral proportion of Vδ1 and Vδ2 subset was detected by FACS after stained with anti δ1-PE and anti δ2-APC. The local colonic proportion of this two subsets were measured under laser confocal fluorescence microscope. Vδ1 γδ T cells were enriched from the total peripheral T cells by minoantibody-immuno-microbeads (MACS) method. and cultured, functionally evaluated by CCK-8 assay (proliferation)，CD69/CD62L molecules expression assay (activation) and ELISA (IL-17 production) respectively.Results: 1. Vδ1 γδ T cells significantly increased while Vδ2 γδ T cells remained unchanged in both the peripheral blood and local colonic tissue from PI-IBS patients (P<0.05). 2. When cultured in vitro, the Vδ1 γδ T cells remarkably proliferated, activated and produced IL-17 (P<0.05). Conclusions: Our results suggest that Vδ1 γδ T cells was the predominant γδ T cells subset in both peripheral and intestinal tissue, and was the major IL-17 producing γδ T cells in PI-IBS.

scholarly journals Increased Vδ1 γδT cells Predominantly Contributed to IL-17 Production in the Development of Adult Human Post-infectious Irritable Bowel Syndrome

2020 ◽  
Author(s):  
liwei dong ◽  
xiaoning sun ◽  
zhichao ma ◽  
jiao fu ◽  
fujin liu ◽  
...  
Keyword(s):  
T Cells ◽  
Irritable Bowel Syndrome ◽  
Peripheral Blood ◽  
Γδ T Cells ◽  
Γδt Cells ◽  
Peripheral T Cells ◽  
Confocal Fluorescence ◽  
Irritable Bowel ◽  
Post Infectious

Abstract Background: γδT cells play an important role in the mucosa inflammation and immunity-associated disorders. Our previous study reported that γδT cells producing IL-17 were involved in the pathogenesis of post-infectious irritable bowel syndrome (PI-IBS). However, their subset characteristic profile in this kind of disease remains unclear. Thus the current study’s aim is to investigate the functionally predominant subset and its role in PI-IBS. Methods: The total T cells were collected from the peripheral blood of patients with PI-IBS. The peripheral proportion of Vδ1 and Vδ2 subset was detected by FACS after stained with anti δ1-PE and anti δ2-APC. The local colonic proportion of this two subsets were measured under laser confocal fluorescence microscope. Vδ1 γδT cells were enriched from the total peripheral T cells by minoantibody-immuno-microbeads (MACS) method. and cultured, functionally evaluated by CCK-8 assay (proliferation)，CD69/CD62L molecules expression assay (activation) and ELISA (IL-17 production) respectively. Results: 1. Vδ1 γδ T cells significantly increased while Vδ2 gd T cells remained unchanged in both the peripheral blood and local colonic tissue from PI-IBS patients (P<0.05). 2. When cultured in vitro, the Vδ1 gd T cells remarkably proliferated, activated and produced IL-17 (P<0.05）. Conclusions: Our results suggest that Vδ1 γδ T cells was the predominant γδ T cells subset in both peripheral and intestinal tissue, and was the major IL-17 producing γδ T cells in PI-IBS.

In vitro adrenergic and glucocorticoid regulation of cytokine production in postinfectious irritable bowel syndrome

2006 ◽  
Vol 20 (3) ◽  
pp. 44
Author(s):  
Ayscha Lucas ◽  
Pieter Cobelens ◽  
Gerald Holtmann ◽  
Guido Gerken ◽  
Sebastian Haag ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Cytokine Production ◽  
Irritable Bowel ◽  
Postinfectious Irritable Bowel Syndrome

scholarly journals Relationship ofCampylobacterToxigenicity In Vitro to the Development of Postinfectious Irritable Bowel Syndrome

2001 ◽  
Vol 184 (5) ◽  
pp. 606-609 ◽  
Author(s):  
Jonathan P. Thornley ◽  
David Jenkins ◽  
Keith Neal ◽  
Trina Wright ◽  
Jo Brough ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Irritable Bowel ◽  
Postinfectious Irritable Bowel Syndrome

Colonic mucosal mediators from patients with irritable bowel syndrome excite enteric cholinergic motor neurons

2012 ◽  
Vol 24 (12) ◽  
pp. 1118-e570 ◽  
Author(s):  
B. Balestra ◽  
R. Vicini ◽  
C. Cremon ◽  
L. Zecchi ◽  
G. Dothel ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Motor Neurons ◽  
Irritable Bowel

scholarly journals Effect of Breadmaking Process on In Vitro Gut Microbiota Parameters in Irritable Bowel Syndrome

PLoS ONE ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. e111225 ◽  
Author(s):  
Adele Costabile ◽  
Sara Santarelli ◽  
Sandrine P. Claus ◽  
Jeremy Sanderson ◽  
Barry N. Hudspith ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Gut Microbiota ◽  
Irritable Bowel

scholarly journals Sensitive Skins May Be Neuropathic Disorders: Lessons from Studies on Skin and Other Organs

Cosmetics ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 14
Author(s):  
Laurent Misery
Keyword(s):  
Irritable Bowel Syndrome ◽  
Nerve Ending ◽  
Sensitive Skin ◽  
Sensory Disorders ◽  
Small Fiber ◽  
Irritable Bowel

Sensitive skin can be considered a neuropathic disorder. Sensory disorders and the decrease in intra-epidermal nerve ending density are strong arguments for small-fiber neuropathies. Sensitive skin is frequently associated with irritable bowel syndrome or sensitive eyes, which are also considered neuropathic disorders. Consequently, in vitro co-cultures of skin and neurons are adequate models for sensitive skin.

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