scholarly journals Postulation of Leaf Rust Resistance Genes in Seven Chinese Spring Wheat Cultivars

2013 ◽  
Vol 12 (9) ◽  
pp. 1580-1588 ◽  
Author(s):  
Li-hong SHI ◽  
Na ZHANG ◽  
Ya-ya HU ◽  
Xue-jun WEI ◽  
Wen-xiang YANG ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Spring Wheat ◽  
Resistance Genes ◽  
Chinese Spring ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Wheat Cultivars ◽  
Chinese Spring Wheat ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

scholarly journals Characterization of Leaf Rust Resistance in Hard Red Spring Wheat Cultivars

Plant Disease ◽  
2004 ◽  
Vol 88 (10) ◽  
pp. 1127-1133 ◽  
Author(s):  
L. M. Oelke ◽  
J. A. Kolmer
Keyword(s):  
Leaf Rust ◽  
Spring Wheat ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Adult Plant ◽  
Wheat Cultivars ◽  
Seedling Leaf ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Leaf rust, caused by Puccinia triticina Eriks., is the most common disease of wheat (Triticum aestivum L.) in the United States and worldwide. The objective of this study was to characterize seedling and adult plant leaf rust resistance in hard red spring wheat cultivars grown in Minnesota, North Dakota, and South Dakota, and postulate the identity of the seedling leaf rust resistance genes in the cultivars. Twenty-six cultivars, near-isogenic lines of Thatcher wheat that differ for single leaf rust resistance genes, and three wheat cultivars with known leaf rust resistance genes, were tested with 11 different isolates of leaf rust collected from the United States and Canada. The leaf rust infection types produced on seedling plants of the cultivars in greenhouse tests were compared with the infection types produced by the same isolates on the Thatcher near-isogenic lines to postulate which seedling leaf rust resistance genes were present. Seedling leaf rust resistance genes Lr1, Lr2a, Lr10, Lr16, Lr21, and Lr24 were postulated to be present in spring wheat cultivars. Seedling genes Lr3, Lr14a, and Lr23 likely were present in some cultivars but could not be clearly identified in this study. Most of the cultivars had some level of adult plant leaf rust resistance, most likely due to Lr34. Cultivars that had seedling resistance genes Lr1, Lr2a, Lr10, or Lr16 had poor to intermediate levels of leaf rust resistance in field plots. Cultivars with combinations of seedling resistance genes Lr16 and Lr24 with additional adult plant resistance were highly resistant to leaf rust.

scholarly journals Inheritance of Leaf Rust Resistance in Wheat Cultivars Grandin and CDC Teal

Plant Disease ◽  
1997 ◽  
Vol 81 (5) ◽  
pp. 505-508 ◽  
Author(s):  
J. Q. Liu ◽  
J. A. Kolmer
Keyword(s):  
Leaf Rust ◽  
Spring Wheat ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Adult Plant ◽  
Wheat Cultivars ◽  
Hard Red Spring Wheat ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

The hard red spring wheat cultivars Grandin and CDC Teal were genetically examined to determine the number and identity of the leaf rust resistance genes present in both wheats. The two cultivars were crossed with the leaf rust susceptible cultivar Thatcher, and the F1 plants were backcrossed to Thatcher. Fifty-four and 80 BC1F1 plants derived respectively from Grandin and CDC Teal were selfed to produce BC1F2 families. The BC1F2 families were tested as seedlings with isolates of Puccinia recondita f. sp. tritici that differed for virulence to specific leaf rust resistance genes. The BC1F2 families were also tested in the adult-plant stage in field rust nursery tests. Segregation of BC1F2 families in the seedling tests indicated that Grandin had resistance genes Lr2a, Lr3, and Lr10, and was heterogeneous for Lr16. CDC Teal was shown to have the seedling leaf rust gene Lr1. In field rust nursery tests, both Grandin and CDC Teal were shown to have adult-plant resistance genes Lr13 and Lr34. Additional leaf rust resistance genes that condition effective field resistance should be incorporated into hard red spring wheat cultivars to diversify the leaf rust resistance in this wheat class.

scholarly journals Identification of Leaf Rust Resistance Genes in Selected Wheat Cultivars and Development of Multiplex PCR

2019 ◽  
Vol 14 (1) ◽  
pp. 327-334 ◽  
Author(s):  
Agnieszka Tomkowiak ◽  
Roksana Skowrońska ◽  
Alicja Buda ◽  
Danuta Kurasiak-Popowska ◽  
Jerzy Nawracała ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Field Resistance ◽  
Wheat Cultivars ◽  
Pcr Conditions ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

AbstractTen leading wheat cultivars originating from the Plant Breeding and Acclimatization Institute (IHAR) - National Research Institute (Poland) and the Department of Gene Bank (Czech Republic) were used to establish a field experiment in 2017 and 2018 at the Dłoń Experimental Farm. The analyzed wheat genotypes were characterized by diversified field resistance to leaf rust. Jubilatka, Thatcher and Sparta were the most resistant cultivars in field conditions in both 2017 and 2018. The aim of the work was to identify the Lr11, L13, Lr16 and Lr26 genes encoding resistance to leaf rust using molecular SSR markers (wmc24, wmc261, Xgwm630, Xwmc764 and P6M12) and to develop multiplex PCR conditions to accelerate identification of these genes. Markers of three leaf rust resistance genes have been identified simultaneously in these cultivars. Jubilatka, Thatcher and Sparta cultivars may serve as a good source of the analyzed leaf rust resistance genes. In addition, multiplex PCR conditions have been developed for the simultaneous identification of the Lr11 and Lr16 and Lr11 and Lr26 gene pairs.

Registration of Spring Wheat Sources of Leaf Rust Resistance Genes Lr53 , Lr56 , Lr59 , and Lr62

2017 ◽  
Vol 12 (1) ◽  
pp. 157-161
Author(s):  
G. F. Marais ◽  
B. McCallum ◽  
J. A. Kolmer ◽  
S.-M. Pirseyedi ◽  
B. R. Bisek ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Spring Wheat ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

scholarly journals Seedling and Slow Rusting Resistance to Leaf Rust in Chinese Wheat Cultivars

Plant Disease ◽  
2010 ◽  
Vol 94 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Z. F. Li ◽  
X. C. Xia ◽  
Z. H. He ◽  
X. Li ◽  
L. J. Zhang ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Wheat Cultivars ◽  
Slow Rusting ◽  
Cropping Seasons ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Identification of resistance genes is important for developing leaf rust resistant wheat (Triticum aestivum) cultivars. A total of 102 Chinese winter wheat cultivars and advanced lines were inoculated with 24 pathotypes of Puccinia triticina for postulation of leaf rust resistance genes effective at the seedling stage. These genotypes were also planted in the field for characterization of slow rusting responses to leaf rust in the 2006–07 and 2007–08 cropping seasons. Fourteen leaf rust resistance genes—Lr1, Lr2a, Lr3bg, Lr3ka, Lr14a, Lr16, Lr17a, Lr18, Lr20, Lr23, Lr24, Lr26, Lr34, and LrZH84—either singly or in combinations, were postulated in 65 genotypes, whereas known resistance genes were not identified in the other 37 accessions. Resistance gene Lr26 was present in 44 accessions. Genes Lr14a and Lr34 were each detected in seven entries. Lr1 and Lr3ka were each found in six cultivars, and five lines possessed Lr16. Lr17a and Lr18 were each identified in four lines. Three cultivars were postulated to possess Lr3bg. Genes Lr20, Lr24, and LrZH84 were each present in two cultivars. Each of the genes Lr2a and Lr23 may exist in one line. Fourteen genotypes showed slow leaf rusting resistance in two cropping seasons.

scholarly journals Evaluation of Wheat Germplasm for Resistance to Leaf Rust (Puccinia triticina) and Identification of the Sources of Lr Resistance Genes Using Molecular Markers

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1484
Author(s):  
Alma Kokhmetova ◽  
Shynbolat Rsaliyev ◽  
Makpal Atishova ◽  
Madina Kumarbayeva ◽  
Angelina Malysheva ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Spring Wheat ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Seedling Resistance ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Leaf rust, caused by Puccinia triticina (Ptr), is a significant disease of spring wheat spread in Kazakhstan. The development of resistant cultivars importantly requires the effective use of leaf rust resistance genes. This study aims to: (i) determine variation in Ptr population using races from the East Kazakhstan, Akmola, and Almaty regions of Kazakhstan; (ii) examine resistance during seedling and adult plant stages; and (iii) identify the sources of Lr resistance genes among the spring wheat collection using molecular markers. Analysis of a mixed population of Ptr identified 25 distinct pathotypes. Analysis of these pathotypes using 16 Thatcher lines that are near-isogenic for leaf rust resistance genes (Lr) showed different virulence patterns, ranging from least virulent “CJF/B” and “JCL/G” to highly virulent “TKT/Q”. Most of the pathotypes were avirulent to Lr9, Lr19, Lr24, and Lr25 and virulent to Lr1, Lr2a, Lr3ka, Lr11, and Lr30. The Ptr population in Kazakhstan is diverse, as indicated by the range of virulence observed in five different races analyzed in this study. The number of genotypes showed high levels of seedling resistance to each of the five Ptr races, thus confirming genotypic diversity. Two genotypes, Stepnaya 62 and Omskaya 37, were highly resistant to almost all five tested Ptr pathotypes. Stepnaya 62, Omskaya 37, Avangard, Kazakhstanskaya rannespelaya, and Kazakhstanskaya 25 were identified as the most stable genotypes for seedling resistance. However, most of the varieties from Kazakhstan were susceptible in the seedling stage. Molecular screening of these genotypes showed contrasting differences in the genes frequencies. Among the 30 entries, 22 carried leaf rust resistance gene Lr1, and two had Lr9 and Lr68. Lr10 and Lr28 were found in three and four cultivars, respectively. Lr19 was detected in Omskaya 37. Two single cultivars separately carried Lr26 and Lr34, while Lr37 was not detected in any genotypes within this study. Field evaluation demonstrated that the most frequent Lr1 gene is ineffective. Kazakhstanskaya 19 and Omskaya 37 had the highest number of resistance genes: three and four Lr genes, respectively. Two gene combinations (Lr1, Lr68) were detected in Erythrospermum 35 and Astana. The result obtained may assist breeders in incorporating effective Lr genes into new cultivars and developing cultivars resistant to leaf rust.

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