Hypolipidemic effects of aqueous extract ofAcalypha capitata leaves in rats fed on high cholesterol diet

Purpose This study aims to investigate the possible effect of Citrus latifolia (CL) extract on biomarkers of oxidative stress, including lipid peroxidation products in rats fed a high cholesterol diet Design/methodology/approach Hypercholesterolemia was induced by feeding normocholesterolemic rats 1 per cent cholesterol-enriched diet for 15 days. An experimental group (n = 20) was divided into two groups (n = 10) and fed the same diet with or without CL lyophilized aqueous extract (1 per cent) for four weeks. At day 28, ten rats from each group were killed. Findings Treatment with CL lyophilized aqueous extract compared with the untreated group had decreased plasma total cholesterol (TC) (−36 per cent), triacylglycerols (−48 per cent), isoprostanes values (−74 per cent) and reduced thiobarbituric acid reactive substances in erythrocytes (−21 per cent). However, the supplementation of CL peels in the hypercholesterolemic diet enhanced superoxide dismutase (+69 per cent), glutathione reductase (+30 per cent) and catalase activities (+34 per cent). Originality/value In hypercholesterolemic rats, administering CL extract ameliorates dyslipidemia and attenuates lipid peroxidation in tissues. These results suggest that CL could be beneficial in the primary treatment of hypercholesterolemia and oxidative damage caused by a high-cholesterol diet.

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Pharmacological evaluation of aqueous extract of syzigium cumini for its antihyperglycemic and antidyslipidemic properties in diabetic rats fed a high cholesterol diet—Role of PPARγ and PPARα

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2017.02.048 ◽

2017 ◽

Vol 89 ◽

pp. 447-453 ◽

Cited By ~ 37

Author(s):

Sandhya Sharma ◽

Sachchidanand Pathak ◽

Gaurav Gupta ◽

Satish Kumar Sharma ◽

Lalit Singh ◽

...

Keyword(s):

Aqueous Extract ◽

Diabetic Rats ◽

High Cholesterol Diet ◽

Cholesterol Diet ◽

High Cholesterol ◽

Pharmacological Evaluation

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The Effect of Aqueous Extract of Vernonia Amygdalina (Bitter Leaf) on Antioxidants and the Liver of Rabbits on High Cholesterol Diet

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2020/v29i930228 ◽

2020 ◽

pp. 91-100

Author(s):

O. Abdulmalik ◽

O. O. Oladapo

Keyword(s):

Superoxide Dismutase ◽

Aqueous Extract ◽

Fatty Infiltration ◽

Atherogenic Diet ◽

Normal Diet ◽

High Cholesterol Diet ◽

Aspartate Transaminase ◽

Cholesterol Diet ◽

High Cholesterol ◽

Significant Difference

Aims: To determine the effect of V. amygdalina on the levels of antioxidants and the liver of rabbits fed on a atherogenic diet. Study Design: Prospective experimental study. Place and Duration of study: Study lasted 14 weeks at Department of Anatomy, University of Ibadan, Nigeria. Methodology: Eighteen male rabbits were randomly divided into three groups comprising control, high cholesterol diet and high cholesterol diet plus 200mg/kg of aqueous extract of V. amygdalina. The animals were fed a normal animal chow, or a diet supplemented by 0.5% cholesterol and 1% methionine for twelve weeks. Subsequently, serum was obtained for liver function tests. Samples of the liver of the animals were obtained for antioxidant tests and histology. Results: Aspartate transaminase was significantly elevated in atherogenic diet only group compared to animals on normal diet. There was also no statistically significant difference across the three groups in the alanine transaminase values. Superoxide dismutase were significantly reduced in animals that were administered extract. Malondialdehyde was not statistically different across the three groups. The histology of the liver of the animals on high cholesterol diet (with and without extract administration) revealed similar microscopic presence of fatty infiltration of the liver. Conclusion: The use of high cholesterol diet for a duration of twelve weeks in rabbits resulted in significant increased relative liver weight, aspartate transaminase, superoxide dismutase and hepatic microvesicular steatosis. Furthermore, the use of 200mg/kg of aqueous extract of V. amygdalina had ameliorative effect on the level of superoxide dismutase. It did not have a measurable effect on the malondialdehyde, liver enzymes and liver histology.

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Evaluation of in vitro Cholesterol esterase inhibitory and in vivo Anti-hyperlipidemic activity of aqueous extract of Plukenetia conophora Mull. Arg. (Euphorbiaceae)

International Journal of Phytomedicine ◽

10.5138/09750185.2324 ◽

2019 ◽

Vol 11 (1) ◽

Author(s):

Glory Oluremilekun Ajayi ◽

Aleshe Modupe Nofisat ◽

Bassey Mfon Jessica

Keyword(s):

Aqueous Extract ◽

Cholesterol Esterase ◽

High Cholesterol Diet ◽

Phytochemical Analysis ◽

Cholesterol Diet ◽

High Cholesterol ◽

Edible Plant ◽

Standard Drug

Hyperlipidemia is a condition of abnormally high lipids levels in the blood which has been ranked as one of the greatest risk factors contributing to prevalence and severity of coronary heart disease. The available antihyperlipidemic drugs have been associated with some side effects however, herbal management of hyperlipidemia are relatively safe, cheap and readily available. P. conophora is an edible plant consumed in Nigeria as snack and speculated to have beneficial effect on blood lipid profile. The present study evaluates anti-hyperlipidemic effect of aqueous extract of cooked P. conophora nut using in vivo and in vitro experimental models.The anti-hyperlipidemic activity was evaluated using tyloxapol induced-hyperlipidemic rats by intraperitoneal injections of Tyloxapol at a dose of 300 mg/kg body weight and high cholesterol-diet induced rats by oral administration of high cholesterol diet for 60 days. Cholesterol esterase enzyme inhibition was used for the in vitro evaluation.Aqueous extract of P. conophora at varying doses, reduced the elevated lipid parameters in both models; the dose of 500 mg/kg showed comparable hypolipidemic effects with standard drug (Simvastatin) at 10 mg/kg (P<0.01). The extract also inhibited cholesterol esterase enzyme with IC50 value of 129.30±0.10μg/ml while Simvastatin with IC50 value of 51.42±0.13μg/ml. Preliminary phytochemical analysis revealed the presence of; Flavonoids, saponin, cardiac glycoside, alkaloids, tannins, steroids and reducing sugar.P. conophora extract exhibited strong hypolipidemic activity and the dose of 500mg/kg demonstrated equipotent activity as the standard drug; Simvastatin 10mg/kg. The extract also showed inhibitory activity against pancreatic cholesterol esterase enzyme; hence can be used to limit absorption of dietary cholesterol, prevent and treat hyperlipidemia.

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Antihypercholesterolemic and anti-atherogenic effects of lyophilized aqueous extract of Globularia alypum leaves in rats fed a high-cholesterol diet

Nutrition & Food Science ◽

10.1108/nfs-01-2020-0025 ◽

2020 ◽

Vol ahead-of-print (ahead-of-print) ◽

Author(s):

Nawal Taleb-Dida ◽

Djamil Krouf ◽

Yasmina Bahlil ◽

Sarra Dali ◽

Fatima Zohra Alachaher ◽

...

Keyword(s):

Aqueous Extract ◽

Atherogenic Index ◽

High Cholesterol Diet ◽

Standard Diet ◽

Lcat Activity ◽

Cholesterol Diet ◽

High Cholesterol ◽

Apo B ◽

Content Type ◽

Globularia Alypum

Purpose This paper aims to investigate the preventive effects of a concomitant supplementation of a lyophilized aqueous extract of Globularia alypum (Ga) leaves in a high cholesterol-diet (HC-D) on lipid profile and lecithin cholesterol acyltransferase (LCAT) activity in hypercholesterolemic rats. Design/methodology/approach Twenty-four male Wistar rats weighing 232 ± 10 g were divided into four groups (n = 6). Two control groups were fed a standard-diet (St-D) supplemented (C-Ga) or not (C) with 1.66% Ga leaf extract. The two others experimental groups were fed HC-D, which contains the St-D plus 1% of cholesterol and 0.5% of cholic acid supplemented (HC-Ga) or not (HC) with the same amount of Ga. At d28, feces were collected and fasting rats were anesthetized; bloods and livers were removed to measure biochemical parameters. Findings In hypercholesterolemic (HC) rats, Ga supplementation in HC-D induced a significant reduction in ALT (−64%, p = 0.002) and AST (−71%; p = 0.005) activities, in plasma TC (−55%; p = 0.03) and TG (−54%; p = 0.01) concentrations, in cholesterol contents of atherogenic lipoproteins VLDL (−78%; p = 0.004) and LDL-HDL1 (−64%; p = 0.003) and inversely, an increase in those of anti-atherogenic HDL2 (+14%; p = 0.002). Feeding the HC-D-Ga exhibited a reduction in atherogenic index Apo B/Apo A-I (−72%; p = 0.002), an increase in faecal lipids, cholesterol excretion and in plasma apo A-I (+60%; p = 0.002) and HDL2-cholesteryl esters (+32%, p = 0.04) and then improved LCAT activity (+31%; p = 0.03). Originality/value In hypercholesterolemic rats, Globularia alypum extract was effective in preventing lipid disorders by its hypolipidemic action, had an anti-atherogenic potential and a protective effect against cardiovascular risk by enhancing LCAT activity.

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