scholarly journals Cytotoxicity of methanol extracts of Elaeis guineensis on MCF-7 and Vero cell lines

2012 ◽  
Vol 2 (10) ◽  
pp. 826-829 ◽  
Author(s):  
Soundararajan Vijayarathna ◽  
Sreenivasan Sasidharan
Keyword(s):  
Vero Cell ◽  
Cell Lines ◽  
Elaeis Guineensis ◽  
Methanol Extracts ◽  
Vero Cell Lines ◽  
Mcf 7

scholarly journals Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts fromEupatorium odoratum In Vitro: Effects on MCF-7 and Vero Cell Lines

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Faizah Bt. Harun ◽  
Syed Mohsin Syed Sahil Jamalullail ◽  
Khoo Boon Yin ◽  
Zulkhairi Othman ◽  
Anita Tilwari ◽  
...  
Keyword(s):  
Cell Death ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Vero Cell ◽  
Cell Lines ◽  
Autophagic Cell Death ◽  
Biologically Active ◽  
Vero Cell Lines ◽  
Mcf 7 ◽  
Ethyl Acetate Extracts

Eupatorium odoratum (EO)contains many biologically active compounds, the anticancer effects of which are not well documented. This study evaluates the cytotoxic effects and mechanism of action ofEOextracts on MCF-7 and Vero cell lines. Evaluation of the cytotoxic activity using MTT assay, morphological alterations, and apoptosis were carried out. Autophagy was evaluated by LC3-A protein expression. Cytotoxic activity, membrane blebbing and ballooning at 24 hours, replacement by mass vacuolation, and double membrane vesicles mimicking autophagy and cell death were observed in the cancer cells. No apoptosis was observed by DNA fragmentation assay. Overexpression of LC3-A protein indicated autophagic cell death. Cell cycle analysis showed G0 and G2/M arrest. The Vero cells did not show significant cell death at concentrations <100 μg/mL. These results thus suggest that acetone and ethyl acetate extracts ofEOinduce cell death through induction of autophagy and hold potential for development as potential anticancer drugs.

Synthesis, In Silico and In Vivo Evaluation of Novel 1, 3, 4-Thiadiazole Analogues as Novel Anticancer Agents

2020 ◽  
Vol 17 (4) ◽  
pp. 434-444 ◽  
Author(s):  
Swathi Krishna ◽  
Byran Gowramma ◽  
Manal Mohammed ◽  
Rajagopal Kalirajan ◽  
Lakshman Kaviarasan ◽  
...  
Keyword(s):  
Vero Cell ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Binding Interaction ◽  
Standard Drug ◽  
Discovery Studio ◽  
Vero Cell Lines ◽  
Mcf 7

Background: 1,3,4-thiadiazole is a lead molécule which is versatile for a wide variety of biological activities and in continuation of our interest in establishing some novel heterocyclic compounds for antitumor activity. Objective: The objective of the study was to synthesize series of 5-(1,3-benzodioxol-5-yl)-1,3,4- thiadiazol-2-amine derivative and evaluated for their possible in vitro and in vivo anticancer activity. Methods: The synthesis of 2-aminonaphthoxy-1,3,4-thiadiazole and 5-(1,3-benzodioxol-5-yl)-1,3,4- thiadiazol-2-amine as intermediates were carried out by cyclization method. A mixture of thiosemicarbazide and naphthoxyacetic acid/piperonylic acid and phosphoryl chloride were subjected to cyclization with phosphorous oxychloride to obtain compound 3. Further compounds 1 and 3 were reacted with different aromatic aldehydes in methanol to form compounds 2a-e and 4a-e. The compounds 2a-e and 4a-e were characterized for the melting points, IR, Proton NMR and Mass spectra. The compounds were further evaluated for their anticancer activity. The docking study was performed using Discovery studio 4.1 (Accelrys) software against DNA-binding domain of STAT3. The compounds were analyzed for the ligand-protein binding interaction(s) by molecular docking into the active site of STAT3β using the CDOCKER protocol of Discovery studio (v4.1). Results: The title compounds were screened for in vitro anticancer on human breastadenocarcinoma cells (MCF-7 and Vero). Compounds 4c, 4d and 2d against MCF 7 and 4d against Vero cell lines were found to be the most active dérivatives with IC50 values of 1.03, 2.81 and 3.45 µg/ml against MCF 7 and 31.81 µg/ml against Vero cell lines, respectively. Conclusion: From the in vivo anticancer studies, it was concluded that the synthesized compounds 4c and 4d displayed anticancer activity comparable to the standard drug, while the rest of the compounds demonstrated mild potency for anticancer studies.

Cytotoxic effects of Tetraselmis suecica chloroform extracts with silver nanoparticle co-application on MCF-7, 4 T1, and Vero cell lines

2019 ◽  
Vol 32 (1) ◽  
pp. 127-143 ◽  
Author(s):  
Hanaa Ali Hussein ◽  
Habsah Mohamad ◽  
Maziah Mohd Ghazaly ◽  
A. A. Laith ◽  
Mohd Azmuddin Abdullah
Keyword(s):  
Vero Cell ◽  
Cell Lines ◽  
Silver Nanoparticle ◽  
Tetraselmis Suecica ◽  
Cytotoxic Effects ◽  
Vero Cell Lines ◽  
Mcf 7

scholarly journals Whole-Genome Sequences of Zika Virus FLR Strains after Passage in Vero or C6/36 Cells

2018 ◽  
Vol 6 (4) ◽  
Author(s):  
Lindsey A. Moser ◽  
Lauren M. Oldfield ◽  
Nadia Fedorova ◽  
Vinita Puri ◽  
Susmita Shrivastava ◽  
...  
Keyword(s):  
Vero Cell ◽  
Cell Lines ◽  
Virus Strain ◽  
Zika Virus ◽  
Whole Genome ◽  
Genome Sequences ◽  
Complete Genomes ◽  
Vero Cell Lines

ABSTRACT We report 26 complete genomes of Zika virus (ZIKV) isolated after passaging the Zika virus strain FLR in mosquito (C6/36) and mammalian (Vero) cell lines. The consensus ZIKV genomes we recovered show greater than 99% nucleotide identify with each other and with the FLR strain used as input.

Effect of Different Hydatid Cyst Molecules on Hela and Vero Cell Lines Growth In vitro

2013 ◽  
Vol 02 (01) ◽  
Author(s):  
Nasir Aref ◽  
Hedayatollah Shirzad ◽  
Morteza Yousefi ◽  
Hossein Yousofi.Darani
Keyword(s):  
Hydatid Cyst ◽  
Vero Cell ◽  
Cell Lines ◽  
Vero Cell Lines

scholarly journals Growth and Maintenance of Vero Cell Lines

2008 ◽  
Vol 11 (1) ◽  
Author(s):  
Nicole C. Ammerman ◽  
Magda Beier‐Sexton ◽  
Abdu F. Azad
Keyword(s):  
Vero Cell ◽  
Cell Lines ◽  
Vero Cell Lines
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