P17: Analytical evaluation of newly developed enzymelinked immunosorbent assay kit for the detection of tramadol and the metabolite O-desmethyl tramadol in urine and blood

2014 ◽  
Vol 26 (2) ◽  
pp. S37-S38
Author(s):  
L. Stewart ◽  
J. Darragh ◽  
M.E. Benchikh ◽  
M.L. Rodriguez ◽  
R.I. McConnell ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Analytical Evaluation ◽  
Enzymelinked Immunosorbent Assay

Staphylococcus aureus

1991 ◽  
Vol 74 (4) ◽  
pp. 706-710 ◽  
Author(s):  
Merlin S Bergdoll
Keyword(s):  
Staphylococcus Aureus ◽  
Immunosorbent Assay ◽  
Analytical Methods ◽  
Latex Agglutination ◽  
Staphylococcal Enterotoxins ◽  
Strain Testing ◽  
Enzymelinked Immunosorbent Assay ◽  
Gel Diffusion

Abstract The analytical methods for the detection of the staphylococcal enterotoxins can be divided into 2 categories: (1) methods for detection of enterotoxin-producing staphylococcal strains; (2) methods for detection of enterotoxin in foods. Gel diffusion methods (Ouchterlony, microslide), in which the enterotoxin produced by any given strain is compared to one of the identified enterotoxins, are used most frequently for strain testing. The sensitivity of these methods is from 0.1 to 0.5 μg enterotoxin/mL, which is normally adequate to determine the enterotoxigenicity of strains. The methods for the detection of enterotoxin in foods need to be much more sensitive to detect less than 1 ng of enterotoxin/g of food that may be present. The radioimmunoassay (RIA), the enzymelinked immunosorbent assay (ELISA), and the reversed passive latex agglutination (RPLA) method have the necessary sensitivity to detect 1 ng/g of enterotoxin in foods without the use of complicated extraction-concentration procedures. Kits based on the ELISA and RPLA methods are now available commercially for the detection of enterotoxins in foods. Tests have shown that the ELISA methods are somewhat more sensitive than the RPLA method.

scholarly journals PENGARUH OLAHRAGA SELAMA PUASA TERHADAP HORMON MELATONIN PADA TIKUS PUTIH JANTAN JENIS WISTAR

2019 ◽  
Vol 9 (1) ◽  
pp. 84
Author(s):  
Emilia Jefanggi Lolita Widiantini ◽  
Sugiharto Sugiharto ◽  
Olivia Andiana
Keyword(s):  
Time Series ◽  
Immunosorbent Assay ◽  
Series Design ◽  
Time Series Design ◽  
Enzymelinked Immunosorbent Assay

Abstrak:Rendahnya kadar hormon melatonin mengakibatkan insomnia,penyakit kardiovaskular dan alzheimer.Atas dasar itu maka penelitianbertujuan untuk mengetahui pengaruh puasa dengan olahraga terhadaphormon melatonin pada minggu pertama, kedua, ketiga dan keempat.Penelitian ini menggunakan binatang coba tikus putih jenis wistarsebanyak 84 ekor berusia 1 bulan dengan berat badan 50-80gram.Penelitian menggunakan desain randomize true exsperiment controlgroup time series design. Pengumpulan data dilakukan pada minggu 1,minggu 2, minggu 3 dan minggu 4. Pada penelitian ini hewan coba dibagimenjadi 3 kelompok perlakuan yaitu kontrol, puasa dan puasa denganolahraga moderat. Olahraga moderat dilakukan dengan cara lari di atastreadmill khusus tikus dengan dengan kecepatan 14-16 m/menitselama 30menit. Olahraga moderat dilakukan 3 kali/minggu. Sedangkan, puasadilakukan selama 12 jam dimulai dari pukul 18.00 WIB (sahur)-06.00WIB (buka). Pengumpulan sampel darah untuk pemeriksaan melatonindilakukan setelah binatang coba dipuasakan 12 jam, sedangkananalisissampel darah untuk pengukuran melatonin dengan metode ELISA(EnzymeLinked Immunosorbent Assay). Uji analisis melatoninmenggunakan uji beda Anava dengan taraf signifikansi α= 1% atau 0,01.Berdasarkan hasil analisis data terdapat perbedan yang signifikan antarakelompokkadar melatonin pada puasa dan olahraga data bahwa p=002*atau p<0,01 pada kelompok puasa dan p= 0,79 atau p>0,01 padakelompok puasa dan olahraga.Kata kunci:Puasa, Olaharaga Moderat, Hormon Melatonin

scholarly journals El tratamiento con resiniferatoxina exhibe propiedades antiinflamatorias en un modelo murino de inflamación inducida por lipopolisacárido

2021 ◽  
Vol 32 (3) ◽  
pp. 137-146
Author(s):  
José Luis Muñoz-Carrillo ◽  
◽  
Ana María Puebla-Pérez ◽  
Argelia López-Luna ◽  
Juan Armando Flores-De La Torre ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Tnf Α ◽  
Enzymelinked Immunosorbent Assay ◽  
Pg E2 ◽  
Óxido Nítrico

Introducción. La inflamación es una respuesta fisiológica protectora del sistema inmunológico frente a diversos estímulos, tales como la infección o daño celular, la cual, al no resolverse de manera adecuada, puede ser perjudicial para le hospedero. En la búsqueda de nuevas lternativas terapéuticas que inhiban la respuesta inflamatoria, diversos estudios han reportado el uso de varias moléculas, tales como la resiniferatoxina (RTX), un potente agonista del receptor de potencial transitorio vanilloide (TRPV)-1. Objetivo. Evaluar si el tratamiento con RTX exhibe propiedades antiinflamatorias, utilizando un modelo murino de inflamación inducida por lipopolisacárido (LPS). Material y métodos. Se estimularon ratones BALB/c con LPS y posteriormente fueron tratados con dexametasona (DEX), capsaicina (CAP), Bay 11-7082, capsazepina (CPZ) y RTX. Además, otros grupos de ratones fueron estimulados con LPS y luego tratados con Bay 11-7082 y CPZ más RTX. Después del tratamiento, se determinaron cuantitativamente los niveles plasmáticos de prostaglandina (PG)-E2, óxido nítrico (NO), interleucina (IL)-1β y factor de necrosis tumoral (TNF)-α utilizando kits de EnzymeLinked ImmunoSorbent Assay ELISA. Resultados. El tratamiento con RTX disminuyó de manera significativa los niveles plasmáticos de PGE2, NO, IL-1β y TNF-α. Así mismo, se observó que los tratamientos con Bay 11-7082 y CPZ más RTX mostraron un efecto antiinflamatorio sinérgico, observándose una disminución significativa más pronunciada en los niveles plasmáticos de TNF-α y PGE2. Conclusión. Estos hallazgos sugieren que el tratamiento con RTX muestra propiedades antiinflamatorias, aparentemente asociadas con la vía de señalización factor nuclear (NF)-κB, independiente de los receptores TRPV1, colocando a la RTX como un fármaco potencial en el tratamiento de enfermedades inflamatorias.

scholarly journals Detection of enterotoxigenic strains Staphylococcus aureus, producing SEC and SEI, isolated in patients with pneumonia, sepsis and burns

2019 ◽  
pp. 72-78
Author(s):  
F. S. Fluer ◽  
Ya. A. Panova ◽  
A. A. Azanova ◽  
E. V. Mamycheva
Keyword(s):  
Staphylococcus Aureus ◽  
Virulence Factors ◽  
Immunosorbent Assay ◽  
Toxic Shock Syndrome ◽  
Infection Process ◽  
Frequency Of Occurrence ◽  
Toxic Shock ◽  
Lethal Sepsis ◽  
Enzymelinked Immunosorbent Assay ◽  
Severe Intoxication

Aim. To identify the frequency of occurrence of staphylococcal enterotoxins of the SEC and SEI type produced by Staphylococcus aureus strains isolated from patients with different nosology. As you know, the infection process in them proceeds with severe intoxication without vomiting and intestinal disorders.Materials and methods. 79 strains were studied (43 were isolated in case of pneumonia, 13 - in burns, 11 - in sepsis) S. aureus in the presence of SES and SEI using enzymelinked immunosorbent assay (ELISA).Results. It was found that 48.3% of S. aureus strains isolated from patients with pneumonia produced SEC and 72.1% - SEI. The frequency of occurrence of S. aureus strains producing SEC and SEI isolated in patients with burn infections was 23.0 and 15.4%, respectively. 36.4% of staphylococcal strains isolated in patients with sepsis produced SEC, 45.5% - SEI.Discussion. It was found that the proportion of S. aureus cultures producing SEC enterotoxins during sepsis is significantly higher than the strains producing SEB (5.4%) and much smaller than SEA (75.6%). A high percentage of SEI-positive strains was found compared to strains that produce the classic enterotoxins SEA, SEB and SEC isolated from pneumonia. In burn infections, the proportions of strains producing SEC and SEI were 15.4 and 23.0%, respectively, which is significantly lower than SEA (92.9%).Conclusion. The data obtained indicate the need to identify staphylococcus strains that produce both classic and newly discovered enterotoxins, which are crucial virulence factors leading to lethal sepsis, infectious endocarditis and toxic shock syndrome to eliminate them.

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