scholarly journals El tratamiento con resiniferatoxina exhibe propiedades antiinflamatorias en un modelo murino de inflamación inducida por lipopolisacárido

2021 ◽  
Vol 32 (3) ◽  
pp. 137-146
Author(s):  
José Luis Muñoz-Carrillo ◽  
◽  
Ana María Puebla-Pérez ◽  
Argelia López-Luna ◽  
Juan Armando Flores-De La Torre ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Tnf Α ◽  
Enzymelinked Immunosorbent Assay ◽  
Pg E2 ◽  
Óxido Nítrico

Introducción. La inflamación es una respuesta fisiológica protectora del sistema inmunológico frente a diversos estímulos, tales como la infección o daño celular, la cual, al no resolverse de manera adecuada, puede ser perjudicial para le hospedero. En la búsqueda de nuevas lternativas terapéuticas que inhiban la respuesta inflamatoria, diversos estudios han reportado el uso de varias moléculas, tales como la resiniferatoxina (RTX), un potente agonista del receptor de potencial transitorio vanilloide (TRPV)-1. Objetivo. Evaluar si el tratamiento con RTX exhibe propiedades antiinflamatorias, utilizando un modelo murino de inflamación inducida por lipopolisacárido (LPS). Material y métodos. Se estimularon ratones BALB/c con LPS y posteriormente fueron tratados con dexametasona (DEX), capsaicina (CAP), Bay 11-7082, capsazepina (CPZ) y RTX. Además, otros grupos de ratones fueron estimulados con LPS y luego tratados con Bay 11-7082 y CPZ más RTX. Después del tratamiento, se determinaron cuantitativamente los niveles plasmáticos de prostaglandina (PG)-E2, óxido nítrico (NO), interleucina (IL)-1β y factor de necrosis tumoral (TNF)-α utilizando kits de EnzymeLinked ImmunoSorbent Assay ELISA. Resultados. El tratamiento con RTX disminuyó de manera significativa los niveles plasmáticos de PGE2, NO, IL-1β y TNF-α. Así mismo, se observó que los tratamientos con Bay 11-7082 y CPZ más RTX mostraron un efecto antiinflamatorio sinérgico, observándose una disminución significativa más pronunciada en los niveles plasmáticos de TNF-α y PGE2. Conclusión. Estos hallazgos sugieren que el tratamiento con RTX muestra propiedades antiinflamatorias, aparentemente asociadas con la vía de señalización factor nuclear (NF)-κB, independiente de los receptores TRPV1, colocando a la RTX como un fármaco potencial en el tratamiento de enfermedades inflamatorias.

scholarly journals Trifluorobenzamidine prevents allergic rhinitis by regulating IgE, IL-4 and IL-5 in T-cells

2020 ◽  
Vol 19 (5) ◽  
pp. 1023-1029
Author(s):  
Yongbo Zhang ◽  
Zhuo Wu ◽  
Yihui Yang ◽  
Lu Ding
Keyword(s):  
Allergic Rhinitis ◽  
Serum Levels ◽  
Eosinophil Infiltration ◽  
Spleen Weight ◽  
Caspase 1 ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Enzymelinked Immunosorbent Assay ◽  
Inhibitory Potential ◽  
New Treatment

Purpose: To investigate the effect of trifluorobenzamidine (TBI) on a mouse model of ovalbumin (OVA)- induced allergic rhinitis. Methods: Allergic rhinitis was established in mice via sensitization on days 1, 5 and 14 through intraperitoneal injection of OVA (100 μg) in PBS. On day 15, the mice were subjected to intranasal exposure to OVA (1.5 mg dissolved in PBS). Prior to 10 days of intranasal exposure to OVA, the micewere treated with TBI at doses of 5, 10 and 20 μg/kg. Cytokine levels were determined using enzymelinked immunosorbent assay (ELISA) kits, while cyclooxygenase (COX)-2 and caspase-1 activity were assayed with western blotting. Results: Treatment with TBI significantly (p < 0.05) reduced OVA-mediated increases in nasal rub scores, and decreased serum levels of IgE, TNF-α, thymic stromal lymphopoietin (TSLP), IL-1β and histamine in mice. It also significantly regulated spleen weight and IL-4 secretion (p < 0.05) in OVAadministered mice. TBI significantly downregulated the expressions of IL-5, IL-13, TNFα, TSLP, IL-1β and IL-6 (p < 0.05). Administration of TBI caused a marked reduction in OVA-mediated increase in caspase-1 activity in mice intranasal tissues, and also significantly reduced OVA-induced excessive production of MIP-2 and ICAM-1 (p < 0.05). Moreover, TBI prevented OVA-induced infiltration of eosinophils and mast cells into intranasal tissues (p < 0.05). Conclusion: TBI reduces levels of IgE and various pro-inflammatory cytokines in OVA-administered mice. It also regulates Th1:Th2 ratio, inhibited activity of caspase-1, suppressed mast cell/eosinophil infiltration and reduced ICAM-1 and MIP-2 levels. Therefore, TBI possesses inhibitory potential against rhinitis allergy, and thus can potentially be developed as a new treatment strategy for asthma. Keywords: Trifluorobenzamidine, Anti-inflammation, Allergic rhinitis, Cytokines, Caspase-1, Itching

Staphylococcus aureus

1991 ◽  
Vol 74 (4) ◽  
pp. 706-710 ◽  
Author(s):  
Merlin S Bergdoll
Keyword(s):  
Staphylococcus Aureus ◽  
Immunosorbent Assay ◽  
Analytical Methods ◽  
Latex Agglutination ◽  
Staphylococcal Enterotoxins ◽  
Strain Testing ◽  
Enzymelinked Immunosorbent Assay ◽  
Gel Diffusion

Abstract The analytical methods for the detection of the staphylococcal enterotoxins can be divided into 2 categories: (1) methods for detection of enterotoxin-producing staphylococcal strains; (2) methods for detection of enterotoxin in foods. Gel diffusion methods (Ouchterlony, microslide), in which the enterotoxin produced by any given strain is compared to one of the identified enterotoxins, are used most frequently for strain testing. The sensitivity of these methods is from 0.1 to 0.5 μg enterotoxin/mL, which is normally adequate to determine the enterotoxigenicity of strains. The methods for the detection of enterotoxin in foods need to be much more sensitive to detect less than 1 ng of enterotoxin/g of food that may be present. The radioimmunoassay (RIA), the enzymelinked immunosorbent assay (ELISA), and the reversed passive latex agglutination (RPLA) method have the necessary sensitivity to detect 1 ng/g of enterotoxin in foods without the use of complicated extraction-concentration procedures. Kits based on the ELISA and RPLA methods are now available commercially for the detection of enterotoxins in foods. Tests have shown that the ELISA methods are somewhat more sensitive than the RPLA method.

scholarly journals PENGARUH OLAHRAGA SELAMA PUASA TERHADAP HORMON MELATONIN PADA TIKUS PUTIH JANTAN JENIS WISTAR

2019 ◽  
Vol 9 (1) ◽  
pp. 84
Author(s):  
Emilia Jefanggi Lolita Widiantini ◽  
Sugiharto Sugiharto ◽  
Olivia Andiana
Keyword(s):  
Time Series ◽  
Immunosorbent Assay ◽  
Series Design ◽  
Time Series Design ◽  
Enzymelinked Immunosorbent Assay

Abstrak:Rendahnya kadar hormon melatonin mengakibatkan insomnia,penyakit kardiovaskular dan alzheimer.Atas dasar itu maka penelitianbertujuan untuk mengetahui pengaruh puasa dengan olahraga terhadaphormon melatonin pada minggu pertama, kedua, ketiga dan keempat.Penelitian ini menggunakan binatang coba tikus putih jenis wistarsebanyak 84 ekor berusia 1 bulan dengan berat badan 50-80gram.Penelitian menggunakan desain randomize true exsperiment controlgroup time series design. Pengumpulan data dilakukan pada minggu 1,minggu 2, minggu 3 dan minggu 4. Pada penelitian ini hewan coba dibagimenjadi 3 kelompok perlakuan yaitu kontrol, puasa dan puasa denganolahraga moderat. Olahraga moderat dilakukan dengan cara lari di atastreadmill khusus tikus dengan dengan kecepatan 14-16 m/menitselama 30menit. Olahraga moderat dilakukan 3 kali/minggu. Sedangkan, puasadilakukan selama 12 jam dimulai dari pukul 18.00 WIB (sahur)-06.00WIB (buka). Pengumpulan sampel darah untuk pemeriksaan melatonindilakukan setelah binatang coba dipuasakan 12 jam, sedangkananalisissampel darah untuk pengukuran melatonin dengan metode ELISA(EnzymeLinked Immunosorbent Assay). Uji analisis melatoninmenggunakan uji beda Anava dengan taraf signifikansi α= 1% atau 0,01.Berdasarkan hasil analisis data terdapat perbedan yang signifikan antarakelompokkadar melatonin pada puasa dan olahraga data bahwa p=002*atau p<0,01 pada kelompok puasa dan p= 0,79 atau p>0,01 padakelompok puasa dan olahraga.Kata kunci:Puasa, Olaharaga Moderat, Hormon Melatonin

scholarly journals Associação entre os níveis plasmáticos de TNF-α, IFN-γ, IL-10, óxido nítrico e os isotipos de IgG específicos nas formas clínicas da doença de Chagas crônica

2009 ◽  
Vol 42 (4) ◽  
pp. 425-430 ◽  
Author(s):  
Cristina Wide Pissetti ◽  
Dalmo Correia ◽  
Teodoro Braga ◽  
Gladstone Eustáquio Lima Faria ◽  
Rafael Faria de Oliveira ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Tnf Α ◽  
Ifn Γ ◽  
America Latina ◽  
Óxido Nítrico

A doença de Chagas é uma importante doença parasitária crônica, que acomete cerca de 9-11 milhões de pessoas na América Latina. Provavelmente, uma combinação de fatores relacionados ao parasito e ao hospedeiro podem ser os responsáveis pela patogênese na fase crônica da doença. Dentre os fatores relacionados ao hospedeiro, a resposta imunológica é um parâmetro de especial interesse. Objetivamos avaliar os níveis plasmáticos das citocinas interferon gama, interleucina 10, fator de necrose tumoral alfa e das imunoglobulinas G total, 3 e 4, por ELISA e do óxido nítrico, pela reação de Griess, entre indivíduos soronegativos e soropositivos para Trypanosoma cruzi, com as formas clínicas cardíaca, indeterminada e digestiva. Os indivíduos soropositivos para Trypanosoma cruzi produziram níveis significativamente mais elevados de imunoglobulinas G total e G3. Indivíduos com a forma digestiva apresentam níveis mais elevados de imunoglobulina G4 e interleucina 10. Entretanto, tais indivíduos apresentaram menores níveis de óxido nítrico do que controles. Os resultados sugerem que os maiores níveis de IL-10 observados nos indivíduos com a forma digestiva poderiam contribuir com os maiores níveis de IgG4 específicos observados.

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