Study of the polymorphism of ovine αs1- and αs2-caseins by capillary electrophoresis

1997 ◽  
Vol 64 (4) ◽  
pp. 525-534 ◽  
Author(s):  
ISIDRA RECIO ◽  
MERCEDES RAMOS ◽  
LOURDES AMIGO
Keyword(s):  
Capillary Electrophoresis ◽  
Cation Exchange ◽  
Isoelectric Focusing ◽  
Genetic Variants ◽  
Fast Moving ◽  
Capillary Electrophoresis Method ◽  
Automated Separation ◽  
Present Technique ◽  
Electrophoresis Method ◽  
The Common

Polymorphism of ovine αs1-caseins was studied by capillary electrophoresis at pH 3·0±0·1. Individual caseins (CN) were selected according to their genetic variants, as determined by PAGE and isoelectric focusing. The ovine caseins, containing different genetic variants of αs1-CN and αs2-CN, were fractionated by cation-exchange FPLC®. The αs1-CN variants A, B and C, and the fast moving αs2-CN variant were identified by a capillary electrophoresis method. The fast moving αs2-CN variant, so-called for its behaviour in PAGE, also had a faster electrophoretic mobility than the common αs2-CN when analysed by the present technique. The capillary electrophoresis method gave excellent, rapid, automated separation of αs1-CN and αs2-CN variants and was suitable for screening studies.

Study of the polymorphism of caprine milk caseins by capillary electrophoresis

1997 ◽  
Vol 64 (4) ◽  
pp. 515-523 ◽  
Author(s):  
ISIDRA RECIO ◽  
MARIA LUISA PÉREZ-RODRÍGUEZ ◽  
LOURDES AMIGO ◽  
MERCEDES RAMOS
Keyword(s):  
Capillary Electrophoresis ◽  
Liquid Chromatography ◽  
Sample Preparation ◽  
Cation Exchange ◽  
Genetic Polymorphisms ◽  
Quantitative Study ◽  
Milk Proteins ◽  
Capillary Electrophoresis Method ◽  
Caprine Milk ◽  
Electrophoresis Method

Polymorphism of caprine milk proteins was studied by capillary electrophoresis. Identification of casein (CN) fractions was effected by using isolated fractions from cation-exchange fast protein liquid chromatography. Genetic polymorphisms in caprine αs2-CN, αs1-CN, β-CN and κ-CN have been determined. κ-CN A and B, β-CN A and null, αs2-CN A, B and C, αs1-CN A, B, C and null, and other forms with intermediate and low αs1-CN content have been identified. The capillary electrophoresis method made it possible to analyse whole caprine milk using simple sample preparation and was rapid, automated and suitable for phenotyping studies. This method may also permit the quantitative study of different protein fractions.

Capillary electrophoresis method for determination ofD-serine and its application for monitoring of serine racemase activity

2006 ◽  
Vol 27 (13) ◽  
pp. 2558-2566 ◽  
Author(s):  
Dušan Koval ◽  
Jana Jirásková ◽  
Kvido Stříšovský ◽  
Jan Konvalinka ◽  
Václav Kašička
Keyword(s):  
Capillary Electrophoresis ◽  
Serine Racemase ◽  
Capillary Electrophoresis Method ◽  
Electrophoresis Method

Analytical evaluation of a new capillary electrophoresis method for carbohydrate-deficient transferrin measurement

2007 ◽  
Vol 382 (1-2) ◽  
pp. 48-53 ◽  
Author(s):  
François Schellenberg ◽  
Catherine Girre ◽  
Bertrand Nalpas ◽  
Arnaud Plat ◽  
Antonio Tome ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Analytical Evaluation ◽  
Capillary Electrophoresis Method ◽  
Carbohydrate Deficient Transferrin ◽  
Electrophoresis Method
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