Total cercarial output in two populations of Galba truncatula experimentally infected with Fasciola hepatica

2009 ◽  
Vol 84 (1) ◽  
pp. 77-80
Author(s):  
P. Vignoles ◽  
F. Aimeur ◽  
A. Titi ◽  
D. Rondelaud ◽  
A. Mekroud ◽  
...  
Keyword(s):  
Fasciola Hepatica ◽  
Snail Host ◽  
The Other ◽  
Galba Truncatula ◽  
Post Exposure ◽  
Complete Development ◽  
Experimental Infections ◽  
Two Populations

AbstractExperimental infections of pre-adult Galba truncatula (two populations) using one, two, three or five miracidia of Fasciola hepatica per snail were carried out to determine the total number of cercariae produced by snails when they were sacrificed at day 70 post-exposure (at 24°C). When the number of miracidia used for each snail increased at exposure, significant numerical augmentations of live rediae and of intraredial differentiating cercariae were noted. In contrast, only insignificant differences between mean numbers of free cercariae were found. The number of metacercariae produced by each snail (after shedding and/or after dissection) increased in infections with up to three miracidia per snail in each population and strongly decreased in infections with five miracidia per snail. Compared to the other types of infections, the delay of cercarial differentiation noted in the five-miracidia groups was probably due to the volume of the snail host, which would be too small to assure the complete development of five sporocysts.

scholarly journals Differences in the compatibility of infection between the liver flukes Fascioloides magna and Fasciola hepatica in a Colombian population of the snail Galba sp.

2014 ◽  
Vol 89 (6) ◽  
pp. 720-726 ◽  
Author(s):  
G. Dreyfuss ◽  
A.C. Correa ◽  
F.F. Djuikwo-Teukeng ◽  
A. Novobilský ◽  
J. Höglund ◽  
...  
Keyword(s):  
Czech Republic ◽  
Larval Development ◽  
Fasciola Hepatica ◽  
Intermediate Hosts ◽  
The Czech Republic ◽  
Galba Truncatula ◽  
Fascioloides Magna ◽  
Post Exposure ◽  
Experimental Infections ◽  
Liver Flukes

AbstractExperimental infections of Galba sp. (origin, Colombia) with allopatric isolates of Fasciola hepatica from France or Fascioloides magna from the Czech Republic were carried out during five successive snail generations to determine if this lymnaeid might sustain complete larval development of either parasite. In snails exposed to F. hepatica, 7 of 400 snails harboured several rediae and only two snails contained a small number of free cercariae on day 50 post-exposure. In contrast, the intensity of F. magna infection in Galba sp. progressively increased from the F1 to F5 generations. Spontaneous cercarial shedding of F. magna occurred in 7 of 100 Galba sp. belonging to the F5 generation and the number of shed cercariae did not differ significantly from that noted in control Galba truncatula of French origin. Galba sp. from Colombia can be added to the list of potential intermediate hosts for F. magna.

Characteristics of Fasciola hepatica infections in Galba truncatula originating from riverbank populations

2010 ◽  
Vol 85 (1) ◽  
pp. 28-32 ◽  
Author(s):  
P. Vignoles ◽  
D. Rondelaud ◽  
G. Dreyfuss
Keyword(s):  
Fasciola Hepatica ◽  
Acid Soil ◽  
Spring Water ◽  
The Other ◽  
Breeding Method ◽  
Galba Truncatula ◽  
Post Exposure ◽  
Petri Dishes

AbstractFour geographical strains of Galba truncatula living on riverbanks (the first on a sedimentary soil and the other three on an acid soil) were subjected to bimiracidial exposures with Fasciola hepatica to study their aptitude for cercarial shedding and to count metacercariae in snails dissected at day 42 post-exposure. All snails were reared in 14-cm Petri dishes at 24°C, with the same spring water (60–73 mg/l of Ca2+) and the same diet (grass and lettuce leaves). Metacercariae of F. hepatica were noted in the four populations after a cercarial shedding or after snail dissection. However, in spite of the breeding method used, the characteristics of snail infections varied with the origin of each geographical strain. For example, the shell heights of infected snails at day 42 were close to those found for the corresponding adults in the field (6.8–8.0 mm for the population living on the sedimentary soil, but only 4.6–5.5 mm for another strain originating from the acid soil). This variability may be explained by assuming that the diet of these riverbank G. truncatula would be different from that of snails living in swampy meadows. However, another hypothesis based on the influence of snail habitat on the characteristics of snail life cannot be excluded.

Calicophoron daubneyiandFasciola hepatica: characteristics of natural and experimental co-infections of these digeneans in the snailLymnaea glabra

2016 ◽  
Vol 91 (1) ◽  
pp. 1-6 ◽  
Author(s):  
P. Vignoles ◽  
A. Titi ◽  
A. Mekroud ◽  
D. Rondelaud ◽  
G. Dreyfuss
Keyword(s):  
Retrospective Study ◽  
Fasciola Hepatica ◽  
Shell Height ◽  
The Other ◽  
Patent Period ◽  
Galba Truncatula ◽  
Experimental Infections ◽  
Calicophoron Daubneyi

AbstractA retrospective study on differentLymnaea glabrasamples collected from central France between 1993 and 2010 was carried out to determine the prevalence of natural co-infections withCalicophoron daubneyiandFasciola hepatica, and to specify the composition of redial burdens. Experimental infections ofL. glabraperformed during the same period of time were also analysed to study metacercarial production of each digenean in co-infected snails. Controls were naturally or experimentally co-infectedGalba truncatula. In natural co-infections, prevalence was 0.7% inL. glabra(186/25,128) and 0.4% inG. truncatula(137/31,345). Low redial burdens were found in these snails, withF. hepaticarediae significantly more numerous inL. glabrathan inG. truncatula(7.5 per snail instead of 5.2). In contrast, the total numbers ofC. daubneyirediae in both lymnaeids were close to each other (4.3 and 3.0 rediae, respectively). In experimentally co-infected groups, prevalence was greater inG. truncatulathan in the other lymnaeid (6.3% instead of 3.0%). Significantly shorter patent periods and lower metacercarial production for each digenean were noted inL. glabrathan inG. truncatula.However, in both lymnaeids, the two types of cercariae were released during the same shedding waves and several peaks during the patent period were synchronous. In spite of a greater shell height forL. glabra, metacercarial production of both digeneans in co-infected snails was lower than that inG. truncatula, thus indicating a still incomplete adaptation between these FrenchL. glabraand both parasites.

Cercarial productivity of redial generations in single-miracidium infections of Lymnaea truncatula with Paramphistomum daubneyi or Fasciola hepatica

2000 ◽  
Vol 74 (1) ◽  
pp. 1-5 ◽  
Author(s):  
M. Abrous ◽  
D. Rondelaud ◽  
G. Dreyfuss
Keyword(s):  
Fasciola Hepatica ◽  
The Other ◽  
Redial Generation ◽  
Post Exposure ◽  
Laboratory Conditions ◽  
Lymnaea Truncatula

AbstractSingle-miracidium infections of Lymnaea truncatula with Paramphistomum daubneyi or with Fasciola hepatica were carried out under laboratory conditions to count free rediae, their germinal embryos, and to determine the cercarial productivity of each redial generation. In snails infected by P. daubneyi, the cercariae were produced by the first (8.7 cercariae per redia) and second (8.9 per redia) generations. At day 63 post-exposure, they corresponded, respectively, to 53.9% and 46.1% of cercariae produced by all rediae. In snails infected by F. hepatica, the majority of cercariae were produced by the R2a group (18.2 cercariae per redia) and corresponded to 66.0% of cercariae produced all rediae. The cercariae produced by the other redial groups were more limited in number: 17.5 per redia in the R1b group (28.7%) and 2.0 per redia in the R2b/R3a group (5.3%). Cercarial productivity of P. daubneyi until day 63 post-exposure was more limited in number than that of F. hepatica: a total of 145 cercariae per snail versus 427 per snail.

Fasciola gigantica: larval productivity of three different miracidial isolates in the snail Lymnaea truncatula

2003 ◽  
Vol 77 (1) ◽  
pp. 11-14 ◽  
Author(s):  
Y. Dar ◽  
P. Vignoles ◽  
D. Rondelaud ◽  
G. Dreyfuss
Keyword(s):  
Geographic Origin ◽  
Fasciola Gigantica ◽  
The Other ◽  
Redial Generation ◽  
Post Exposure ◽  
Experimental Infections ◽  
Other Hand ◽  
Lymnaea Truncatula ◽  
Egyptian Isolate

AbstractBimiracidial infections of Lymnaea truncatula with three isolates of Fasciola gigantica, originating from China, Egypt and Madagascar, were carried out to determine the effect of geographic origin of the parasite on the larval productivity of redial generations. The prevalences of experimental infections in snails exposed to strains from Madagascar, China and Egypt were 20.8%, 60.0% and 80.0%, respectively. At day 49 post-exposure (p.e.), the total number of free rediae in snails infected with the Egyptian isolate was significantly higher than that recorded in the Madagascan group. On the other hand, at day 49 p.e., the majority of cercariae in the Chinese and Egyptian groups were produced by R2a rediae (70.6% and 66.6% of cercariae produced by all live rediae), while, in the Madagascan group, the cercariae were produced mainly by the first redial generation. Snails infected with the Egyptian isolate of miracidia developed more live rediae and, consequently, could produce a higher number of cercariae. As a result, L. truncatula snails were highly adapted to infections with the Egyptian and Chinese isolates of F. gigantica.

Lymnaea glabra: progressive increase in susceptibility to Fasciola hepatica through successive generations of experimentally infected snails

2014 ◽  
Vol 89 (4) ◽  
pp. 398-403 ◽  
Author(s):  
D. Rondelaud ◽  
F.F. Djuikwo Teukeng ◽  
P. Vignoles ◽  
G. Dreyfuss
Keyword(s):  
Fasciola Hepatica ◽  
Progressive Increase ◽  
Parasite Infection ◽  
Snail Infection ◽  
Cattle Breeding ◽  
Galba Truncatula ◽  
Successive Generations ◽  
Breeding Farms ◽  
Two Populations ◽  
Over Time

AbstractExperimental infections of Lymnaea glabra (two populations) with Fasciola hepatica were carried out during seven successive snail generations, to determine if prevalence and intensity of snail infection increased over time through descendants of snails already infected with F. hepatica. Controls were descendants coming from uninfected parents and infected according to the same protocol. No larval forms were found in the bodies of control snails coming from uninfected parents. In contrast, prevalence and intensity of F. hepatica infection in snails originating from infected parents progressively increased from the F2 or F3 to the F6 generation of L. glabra. In another experiment carried out with the F7 generations of L. glabra and a single generation of Galba truncatula (as controls), the prevalence of F. hepatica infection and the total number of cercariae were lower in L. glabra (without significant differences between both populations). If the number of cercariae shed by infected snails was compared to overall cercarial production noted in snails containing cercariae but dying without emission, the percentage was greater in G. truncatula (69% instead of 52–54% in L. glabra). Even if most characteristics of F. hepatica infection were lower in L. glabra, prevalence and intensity of parasite infection increased with snail generation when tested snails came from infected parents. This mode of snail infection with F. hepatica suggests an explanation for cases of fasciolosis occurring in cattle-breeding farms where paramphistomosis is lacking and G. truncatula is absent.

Redial growth and cercarial productivity of Fasciola hepatica in three species of young lymnaeid snails

2002 ◽  
Vol 76 (3) ◽  
pp. 269-272 ◽  
Author(s):  
P. Vignoles ◽  
G. Dreyfuss ◽  
D. Rondelaud
Keyword(s):  
Body Length ◽  
Significant Influence ◽  
Fasciola Hepatica ◽  
Snail Species ◽  
Post Exposure ◽  
Experimental Infections ◽  
Laboratory Conditions ◽  
The Mean ◽  
Cattle Strain

AbstractExperimental infections of 1-mm high snails using three populations of Lymnaea (L. glabra, L. ovata and L. truncatula) and a cattle strain of Fasciola hepatica miracidia were carried out under laboratory conditions to determine if the snail species had an effect on the number of free rediae, their growth, and cercarial productivity in relation to each redial category (R1a, R1b, R2a, or R2b/R3a). The total number of rediae ranged from 6.4 to 7.5 per snail. The mean body length of rediae varied from 1–1.2 mm (R1a) to 0.3–0.4 mm (R2b/R3a). The width of the intrapharyngeal lumen also varied from 26.0–38.8 μm to 3.0–4.2 μm, respectively. The redial category had a significant effect on both measurements, whereas snail species only had a significant influence on body length. The mean number of cercariae produced by all living rediae at day 49 post-exposure ranged from 63.0 in L. glabra to 87.2 in L. truncatula. In L. ovata and L. truncatula, 55.8% and 58.6% of cercariae, respectively, were produced by R2a rediae, whereas 53.9% of cercariae in L. glabra were formed by the R1b rediae. When young snails were infected with F. hepatica, the species of snail had an effect on the number of living rediae, their length and their cercarial productivity.

The development of rediae ofFasciola hepaticainRadix natalensissubjected twice to bimiracidial exposures

2010 ◽  
Vol 85 (2) ◽  
pp. 210-214 ◽  
Author(s):  
Y. Dar ◽  
P. Vignoles ◽  
G. Dreyfuss ◽  
D. Rondelaud
Keyword(s):  
High Frequency ◽  
Fasciola Hepatica ◽  
Snail Population ◽  
Developmental Patterns ◽  
Post Exposure ◽  
Experimental Infections ◽  
French Isolate ◽  
Over Time

AbstractExperimental infections of EgyptianRadix natalensiswith a French isolate ofFasciola hepatica(each snail was subjected twice to a bimiracidial exposure) were carried out to determine how many sporocysts grew in these snails and to study the developmental patterns of redial generations. Single-sporocyst infections were found in 69.3% (34/49) of infected snails, with equivalent numbers of normal and abnormal patterns. Snails with two- and three-sporocyst infections were 24.4% and 6.1%, respectively. In single- and two-sporocyst infections at days 42 and 56 post-exposure, the total redial burden was significantly higher in snails with a normal redial development. In two- and three-sporocyst infections, the overall maturity of rediae was delayed at days 42 and 56. The high frequency of abnormal patterns inR. natalensis(53.1% of all infected snails showed degeneration of a first mother redia) might be due to incomplete adaptation between the snail population and the parasite. The delayed redial maturity in two- and three-sporocyst infections can mainly be explained by the volume of the snail body, which would be insufficient to allow the simultaneous differentiation of most rediae over time.

The presence of predators modifies the larval development of Fasciola hepatica in surviving Lymnaea truncatula

2002 ◽  
Vol 76 (2) ◽  
pp. 175-178 ◽  
Author(s):  
D. Rondelaud ◽  
P. Vignoles ◽  
G. Dreyfuss
Keyword(s):  
Survival Rate ◽  
Larval Development ◽  
Significant Variation ◽  
Fasciola Hepatica ◽  
Stress Reaction ◽  
Experimental Exposure ◽  
Post Exposure ◽  
Progressive Development ◽  
Experimental Infections ◽  
Lymnaea Truncatula

AbstractExperimental infections of Lymnaea truncatula with Fasciola hepatica were performed to study the consequences of the presence of predators (sciomyzid larvae or zonitid snails) on the characteristics of larval F. hepatica development in surviving snails. Controls consisted of infected snails that were not subjected to predators. Compared to controls, the survival rate at day 30 post-exposure, the duration of cercarial shedding, and the number of cercariae shed by surviving snails were significantly lower when predators were present in snail breeding boxes, whatever the type of predator used. In contrast, the prevalences of Fasciola infections in snails, and the length of time between exposure and the onset of cercarial shedding showed no significant variation. The progressive development of a stress reaction in surviving snails against predators during the first 30 days of experimental exposure to F. hepatica would influence snail survival during the cercarial shedding period and, consequently, the number of cercariae shed by the snails.

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