Molecular and morphological characterization of Belondira coomansi n. sp. (Nematoda: Dorylaimida, Belondiridae) from Iran

2017 ◽  
Vol 92 (5) ◽  
pp. 630-636
Author(s):  
B. Golhasan ◽  
R. Heydari ◽  
E. Miraeiz ◽  
J. Abolafia ◽  
R. Peña-Santiago
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Large Subunit ◽  
Morphological Characterization ◽  
Dna Sequence Data ◽  
Anterior Position ◽  
Genital Branch ◽  
General Size ◽  
A New Species

AbstractA new species belonging to the genus Belondira is described from natural areas in Iran. Belondira coomansi n. sp. is characterized by its general size, the dimensions and appearance of its lip region, presence of distinct labial and post-labial sclerotization, the length of the odontostyle and its inconspicuous lumen and aperture, the length of the neck and its pharyngeal expansion, the reduction of the female anterior genital branch to a simple uterine sac, a very short posterior uterus, the anterior position of the vulva, the length and shape of the caudal region with distinctly thick cuticle at its tip, the length of the spicules, and the presence of only one pair of ventromedian supplements. The new species is close to Belondira brevibulba, B. sacchari, B. tenuidens and B. thornei, and it is compared to them. Molecular characterization (D2–D3 expansion segments of the rRNA large subunit) of the new species is also provided, representing only the second species of this genus for which any DNA sequence data are available.

scholarly journals Molecular and Morphological Characterization of Colaconema formosanum sp. nov. (Colaconemataceae, Rhodophyta)—A New Endophytic Filamentous Red Algal Species from Taiwan

2021 ◽  
Vol 9 (8) ◽  
pp. 809
Author(s):  
Meng-Chou Lee ◽  
Han-Yang Yeh
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Algal Species ◽  
Morphological Characterization ◽  
Bisphosphate Carboxylase ◽  
The Third ◽  
Red Algal ◽  
Southern Taiwan ◽  
A New Species

The genus Colaconema, containing endophytic algae associated with economically important macroalgae, is common around the world, but has rarely been reported in Taiwan. A new species, C. formosanum, was found attached to an economically important local macroalga, Sarcodia suae, in southern Taiwan. The new species was confirmed based on morphological observations and molecular analysis. Both the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) and cytochrome c oxidase subunit I (COI-5P) genes showed high genetic variation between our sample and related species. Anatomical observations indicated that the new species presents asexual reproduction by monospores, cylindrical cells, irregularly branched filaments, a single pyrenoid, and single parietal plastids. Our research supports the taxonomic placement of C. formosanum within the genus Colaconema. This study presents the third record of the Colaconema genus in Taiwan.

Phylogenetic and morphological characterization of Byssosphaeria macarangae sp. nov., and B. taiwanense sp. nov. from Macaranga tanarius

Phytotaxa ◽  
2018 ◽  
Vol 364 (3) ◽  
pp. 211 ◽  
Author(s):  
DANUSHKA SANDARUWAN TENNAKOON ◽  
RAJESH JEEWON ◽  
CHANG HSIN KUO ◽  
KEVIN DAVID HYDE
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
New Taxa ◽  
Sequence Data ◽  
Morphological Characterization ◽  
Sequence Analyses ◽  
Morphological Examination ◽  
Dna Sequence Data ◽  
Macaranga Tanarius

Byssosphaeria species were collected from Macaranga tanarius (Euphorbiaceae) in Shihnong forest area in Chiayi, Taiwan and subjected to morphological examination and DNA sequence analyses. Two new taxa, Byssosphaeria macarangae sp. nov and B. taiwanense sp. nov are herein described. Both species can be distinguished from other Byssosphaeria species based on distinct size differences in asci, ascospores and DNA sequence data. Detailed descriptions, micrographs and phylogenetic data of the new species are provided.

A new Liopeltis Fitzinger, 1843 (Squamata: Colubridae) from Pulau Tioman, Peninsular Malaysia

Zootaxa ◽  
2020 ◽  
Vol 4766 (3) ◽  
pp. 472-484
Author(s):  
HANNAH E. SOM ◽  
L. LEE GRISMER ◽  
PERRY L. JR. WOOD ◽  
EVAN S. H. QUAH ◽  
RAFE M. BROWN ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
New Species ◽  
Dna Sequence ◽  
Rare Species ◽  
Sequence Data ◽  
Peninsular Malaysia ◽  
Tropical Asia ◽  
Dna Sequence Data ◽  
A New Species ◽  
Lines Of Evidence

Liopeltis is a genus of poorly known, infrequently sampled species of colubrid snakes in tropical Asia. We collected a specimen of Liopeltis from Pulau Tioman, Peninsular Malaysia, that superficially resembled L. philippina, a rare species that is endemic to the Palawan Pleistocene Aggregate Island Complex, western Philippines. We analyzed morphological and mitochondrial DNA sequence data from the Pulau Tioman specimen and found distinct differences to L. philippina and all other congeners. On the basis of these corroborated lines of evidence, the Pulau Tioman specimen is described as a new species, L. tiomanica sp. nov. The new species occurs in sympatry with L. tricolor on Pulau Tioman, and our description of L. tiomanica sp. nov. brings the number of endemic amphibians and reptiles on Pulau Tioman to 12. 

A new species of trypanosome, Trypanosoma desterrensis sp. n., isolated from South American bats

Parasitology ◽  
2003 ◽  
Vol 127 (3) ◽  
pp. 265-271 ◽  
Author(s):  
E. C. GRISARD ◽  
N. R. STURM ◽  
D. A. CAMPBELL
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Sequence Data ◽  
Rrna Gene ◽  
South American ◽  
Sequence Variability ◽  
Dna Sequence Data ◽  
Hybridization Probes ◽  
5S Rrna Gene ◽  
A New Species

Trypanosomes isolated from South American bats include the human pathogen Trypanosoma cruzi. Other Trypanosoma spp. that have been found exclusively in bats are not well characterized at the DNA sequence level and we have therefore used the SL RNA gene to differentiate and characterize kinetoplastids isolated from bats in South America. A Trypanosoma sp. isolated from bats in southern Brazil was compared with the geographically diverse isolates T. cruzi marinkellei, T. vespertilionis, and T. dionisii. Analysis of the SL RNA gene repeats revealed size and sequence variability among these bat trypanosomes. We have developed hybridization probes to separate these bat isolates and have analysed the DNA sequence data to estimate their relatedness. A new species, Trypanosoma desterrensis sp. n., is proposed, for which a 5S rRNA gene was also found within the SL RNA repeat.

scholarly journals Helminthosporium velutinum and H. aquaticum sp. nov. from aquatic habitats in Yunnan Province, China

Phytotaxa ◽  
2016 ◽  
Vol 253 (3) ◽  
pp. 179 ◽  
Author(s):  
DAN ZHU ◽  
ZONG-LONG LUO ◽  
DARBHE JAYARAMA BAHT ◽  
ERIC.H.C. MCKENZIE ◽  
ALI H. BAHKALI ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Tree ◽  
Dna Sequence ◽  
Yunnan Province ◽  
Sequence Data ◽  
Its Sequence ◽  
Aquatic Habitats ◽  
Submerged Wood ◽  
Dna Sequence Data ◽  
A New Species

Helminthosporium species from submerged wood in streams in Yunnan Province, China were studied based on morphology and DNA sequence data. Descriptions and illustrations of Helminthosporium velutinum and a new species H. aquaticum are provided. A combined phylogenetic tree, based on SSU, ITS and LSU sequence data, place the species in Massarinaceae, Pleosporales. The polyphyletic nature of Helminthosporium species within Massarinaceae is shown based on ITS sequence data available in GenBank.

Amanita mansehraensis, a new species in section Vaginatae from Pakistan

Phytotaxa ◽  
2019 ◽  
Vol 409 (4) ◽  
pp. 189-201 ◽  
Author(s):  
MALKA SABA ◽  
DANNY HAELEWATERS ◽  
MUHAMMAD FIAZ ◽  
ABDUL NASIR KHALID ◽  
DONALD H. PFISTER
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Large Subunit ◽  
Pine Forests ◽  
Evolutionary Relationships ◽  
Internal Transcribed Spacer Region ◽  
Bayesian Inferences ◽  
A New Species

A new species of Amanita subgenus Amanita sect. Vaginatae is described and illustrated based on material collected in pine forests in district Mansehra, Khyber Pakhtoonkhaw, Pakistan. Amanita mansehraensis is recognized by the presence of a light brown or light greyish olive pileus with strong brown or deep brown pileus center; non-appendiculate, rimose, sulcate or plicate striate pileus margin; subglobose to ellipsoid basidiospores; and a saccate volva. The internal transcribed spacer region (ITS) and large subunit of the nuclear ribosomal RNA gene (nrLSU) were used for the delimitation of this species based on sequence data. The evolutionary relationships of A. mansehraensis with other species of Amanita were inferred by means of Maximum Likelihood and Bayesian inferences of the nrLSU dataset and concatenated ITS+nrLSU dataset. Amanita mansehraensis is most closely related to A. brunneofuliginea, A. pseudovaginata, and the recently described A. glarea.

A new species of big-headed, fanged dicroglossine frog (Genus Limnonectes) from Thailand

Zootaxa ◽  
2008 ◽  
Vol 1807 (1) ◽  
pp. 26 ◽  
Author(s):  
DAVID S. McLEOD
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Sequence Data ◽  
Sequence Divergence ◽  
Type Material ◽  
Base Pairs ◽  
Dna Sequence Data ◽  
16S Gene ◽  
A New Species

A new species of the dicroglossine genus Limnonectes from eastern Thailand and its tadpole are described. Analysis of DNA sequence data from 2518 base-pairs of the mitochondrial 12S and 16S gene regions places the species within the complex of frogs currently referred to as Limnonectes kuhlii and demonstrates it to be a separate lineage (>18% sequence divergence from type-material of L. kuhlii from Java). The new species differs from L. kuhlii by having nuptial pads, a greater snout–vent length, and different relative finger lengths than specimens from Java. It has more extensive toe webbing, a different arrangement of nuptial pads, and a greater snout–vent length than Limnonectes laticeps. The new species, which lacks vocal slits, also can be distinguished from the morphologically similar Limnonectes namiyei from Japan, which possesses vocal slits.

scholarly journals Morphology, genetic characterization and molecular phylogeny of pinworm Skrjabinema longicaudatum n. sp. (Oxyurida: Oxyuridae) from the endangered Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae)

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Yi-Fan Cao ◽  
Hui-Xia Chen ◽  
Yang Li ◽  
Dang-Wei Zhou ◽  
Shi-Long Chen ◽  
...  
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Sister Relationship ◽  
Tibetan Antelope ◽  
A New Species

Abstract Background The Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) is an endangered species of mammal endemic to the Qinghai-Tibetan Plateau. Parasites and parasitic diseases are considered to be important threats in the conservation of the Tibetan antelope. However, our present knowledge of the composition of the parasites of the Tibetan antelope remains limited. Methods Large numbers of nematode parasites were collected from a dead Tibetan antelope. The morphology of these nematode specimens was observed using light and scanning electron microscopy. The nuclear and mitochondrial DNA sequences, i.e. small subunit ribosomal DNA (18S), large subunit ribosomal DNA (28S), internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 (cox1), were amplified and sequenced for molecular identification. Moreover, phylogenetic analyses were performed using maximum likelihood (ML) inference based on 28S and 18S + 28S + cox1 sequence data, respectively, in order to clarify the systematic status of these nematodes. Results Integrated morphological and genetic evidence reveals these nematode specimens to be a new species of pinworm Skrjabinema longicaudatum (Oxyurida: Oxyuridae). There was no intraspecific nucleotide variation between different individuals of S. longicaudatum n. sp. in the partial 18S, 28S, ITS and cox1 sequences. However, a high level of nucleotide divergence was revealed between the new species and its congeners in 28S (8.36%) and ITS (20.3–23.7%) regions, respectively. Molecular phylogenetic results suggest that the genus Skrjabinema should belong to the subfamily Oxyurinae (Oxyuroidea: Oxyuridae), instead of the subfamily Syphaciidae or Skrjabinemiinae in the traditional classification, as it formed a sister relationship to the genus Oxyuris. Conclusions A new species of pinworm Skrjabinema longicaudatum n. sp. (Oxyurida: Oxyuridae) is described. Skrjabinema longicaudatum n. sp. represents the first species of Oxyurida (pinworm) and the fourth nematode species reported from the Tibetan antelope. Our results contribute to the knowledge of the species diversity of parasites from the Tibetan antelope, and clarify the systematic position of the genus Skrjabinema.

scholarly journals A new species of the blind cave gudgeon Milyeringa (Pisces: Gobioidei, Eleotridae) from Barrow Island, Western Australia, with a redescription of M. veritas Whitley

Zootaxa ◽  
2013 ◽  
Vol 3616 (2) ◽  
pp. 135-150 ◽  
Author(s):  
HELEN K. LARSON ◽  
RALPH FOSTER ◽  
WILLIAM F. HUMPHREYS ◽  
MARK I. STEVENS
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Western Australia ◽  
Sequence Data ◽  
Morphological Data ◽  
Unique Form ◽  
Dna Sequence Data ◽  
The Third ◽  
Blind Cave ◽  
A New Species

A new species of the eyeless eleotrid genus Milyeringa is described from wells sunk on Barrow Island, Western Australia. Milyeringa justitia n. sp. is the third species of the genus to be named. Morphological data and cytochrome c oxidase subunit I (COI) DNA sequence data from a wide sample of localities at which the genus occurs was used to evaluate relationships and species limits. Milyeringa veritas is redescribed, and M. brooksi is synonymised with M. veritas. The unique form and ecology of these fishes, plus the threats to their survival, warrants immediate and continuing attention in management.

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