scholarly journals Differential diagnosis of vesicular stomatitis and foot-and-mouth disease. Examination of virus samples from Mexico with special reference to complement fixation

1949 ◽  
Vol 47 (4) ◽  
pp. 384-389 ◽  
Author(s):  
J. B. Brooksby
Keyword(s):  
Differential Diagnosis ◽  
Special Reference ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
The Other ◽  
Practical Applications ◽  
Foot And Mouth ◽  
Vesicular Stomatitis

The usefulness of the complement-fixation test in differential diagnosis between vesicular stomatitis and foot-and-mouth disease has been demonstrated on two ‘field’ specimens of virus from Mexico. Examples are given of the practical applications of the other methods for this differential diagnosis.

scholarly journals Titration of the virus of foot-and-mouth disease in culture

1954 ◽  
Vol 52 (1) ◽  
pp. 87-99 ◽  
Author(s):  
J. B. Brooksby ◽  
Ella Wardle
Keyword(s):  
Technical Assistance ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Foot And Mouth Disease ◽  
Virus Multiplication ◽  
Mouth Disease ◽  
Fixation Test ◽  
Epithelial Tissue ◽  
Foot And Mouth

A technique is presented for the titration of the virus of foot-and-mouth disease in culture in surviving epithelial tissue from the tongues of cattle. The cultures are incubated in cups on Perspex plates, and the detection of virus multiplication is by a complement-fixation test made on the culture in each cup.On the basis of comparative titrations in culture and in cattle, the method has been found to be as sensitive for the detection of virus as the titration by intradermal inoculation of the tongue of cattle. The method can also be applied in the detection of antibody in neutralization tests.We wish to record our thanks to Messrs E. Scoates and P. Mitchell for their technical assistance, and to Messrs H. M. Smith, R. H. Compton and R. L. Jackson for their part in the design and fabrication of various bottle rotators, plate shakers, Perspex lids and the inoculating box.

scholarly journals The Reaction of Complement Fixation in Foot-and-Mouth Disease as a means of identifying the Different Types of Virus

1929 ◽  
Vol 28 (4) ◽  
pp. 325-339 ◽  
Author(s):  
A. Ciuca
Keyword(s):  
Complement Fixation ◽  
Clinical Course ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
The Other ◽  
Passive Immunisation ◽  
Active Immunity ◽  
Satisfactory Method ◽  
Different Types ◽  
Foot And Mouth

Notwithstanding several efforts, a satisfactory method of serological diagnosis of foot-and-mouth disease has not been arrived at. This uncertainty of serological methods delayed the discovery of the plurality of type of the virus, although the observations of epizootics and the irregular results obtained by active and passive immunisation suggested multiplicity of type. Stimulated by such observations, Vallée and Carré (1922) experimented with two viruses of foot-and-mouth disease, one of which was of German and the other of French origin. They were able to show by experimental infection, confirmed by subsequent testing for active immunity, that cases of foot-and-mouth disease with the same clinical course and symptoms could be caused by two distinct types of virus. These they called A (Allemand) and O (Oise). The only difference between these two types was their inability to produce reciprocal immunity. The existence of more than one type of virus has been confirmed in England by Stockman and Minett (1926), and by Bedson, Maitland and Burbury (1927); in Germany, by Waldmann and Trautwein (1926), and Trautwein (1927); in France, by Lebailly (1926), Olitsky (1927), and in Sweden, by Magnusson and Hermansson (1926).

scholarly journals Vesicular stomatitis and foot-and-mouth disease: analysis of mixed infection in cattle

1952 ◽  
Vol 50 (3) ◽  
pp. 394-404 ◽  
Author(s):  
J. B. Brooksby
Keyword(s):  
Mixed Infection ◽  
Technical Assistance ◽  
Complement Fixation ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mixed Infections ◽  
Foot And Mouth ◽  
Vesicular Stomatitis ◽  
Cross Immunity ◽  
Disease Analysis

1. It has been found possible to detect traces of the virus of foot-and-mouth disease in mixtures of this virus and that of vesicular stomatitis by cross-immunity tests in cattle. A quantity of the virus of foot-and-mouth disease as small as 1·3 I.D. 50 was revealed by the inoculation of such mixtures into cattle immune to vesicular stomatitis.2. It is suggested that such a method would be useful in the analysis of possible mixed infections in field outbreaks.3. The usefulness of complement-fixation tests to confirm the resolution of mixtures has been demonstrated.The author wishes to thank the Director of this Institute, Dr I. A. Galloway, for his advice and encouragement. The technical assistance of Messrs E. Scoates and P. M. Mitchell is also gratefully acknowledged.

Rapid and differential diagnosis of foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis by a new multiplex RT-PCR assay

2008 ◽  
Vol 147 (2) ◽  
pp. 301-311 ◽  
Author(s):  
Jovita Fernández ◽  
Montserrat Agüero ◽  
Luis Romero ◽  
Carmen Sánchez ◽  
Sándor Belák ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Foot And Mouth ◽  
Vesicular Stomatitis ◽  
Vesicular Disease

scholarly journals A non-specific complement-fixing system in foot-and-mouth disease and experiments on the absorption of heterologous antibody from serum

1956 ◽  
Vol 54 (3) ◽  
pp. 328-334 ◽  
Author(s):  
J. B. Brooksby ◽  
S. Erichsen
Keyword(s):  
Complement Fixation ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Foot And Mouth ◽  
Heterologous Antibody

Stored mixtures of the virus and corresponding antisera in foot-and-mouth disease have equal complement-fixing activity whether they are prepared from virus and serum of homologous or heterologous types. On ultracentrifugation the complement-fixing activity is removed from the mixture and the remaining antibody is more sharply type-specific in routine complement-fixation tests than the original serum.

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