scholarly journals Application of the enzyme linked immunosorbent assay to the detection and identification of foot-and-mouth disease viruses

1979 ◽  
Vol 83 (3) ◽  
pp. 513-519 ◽  
Author(s):  
J. R. Crowther ◽  
E. M. E. Abu Elzein
Keyword(s):  
Tissue Culture ◽  
Immunosorbent Assay ◽  
Complement Fixation ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Detection ◽  
Fmd Virus ◽  
Detection And Identification ◽  
Foot And Mouth

An indirect enzyme linked immunosorbent assay (ELISA) was applied to the detection and identification of foot-and-mouth disease (FMD) virus types. The test proved successful for the specific detection of virus from infected tissue culture, and from epithelial tissues from bovines suspected of having FMD. The ELISA compared favourably with the complement fixation (CF) test, being more sensitive and unaffected by anticomplementary factors.

scholarly journals Foot-and-Mouth Disease Virus Typing by Complement Fixation and Enzyme-Linked Immunosorbent Assay using Monovalent and Polyvalent Antisera

1992 ◽  
Vol 4 (3) ◽  
pp. 249-253 ◽  
Author(s):  
Albino Alonso ◽  
Mauricio A. Martins ◽  
D. Gomes Maria da Penha ◽  
Rossana Allende ◽  
Magnus S. Söndahl
Keyword(s):  
Cell Culture ◽  
Immunosorbent Assay ◽  
Complement Fixation ◽  
Detection System ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fmd Virus ◽  
Mouth Disease Virus ◽  
Foot And Mouth

An indirect “sandwich” enzyme-linked immunosorbent assay (ELISA) using polyvalent and monovalent antisera was compared with the 50% complement fixation (CF) test for the detection of foot-and-mouth disease (FMD) O, A, and C virus types. ELISA was more sensitive than CF tests when polyvalent antisera were used for detecting the 3 types of virus in epithelial samples, whereas ELISA using monovalent antisera was the least sensitive technique. The ELISA performed with polyvalent antisera was 9 times more sensitive for detecting FMD virus than that with monovalent antisera. However, viral isolation in cell culture was the most sensitive detection system. The combined use of ELISA with polyvalent antisera and cell culture inoculations was the most effective procedure for identifying FMD virus in epithelial samples from the field.

scholarly journals The specific detection of foot-and-mouth disease virus whole particle antigen (140S) by enzyme labelled immunosorbent assay

1979 ◽  
Vol 83 (1) ◽  
pp. 127-134 ◽  
Author(s):  
E. M. E. Abu Elzein ◽  
J. R. Crowther
Keyword(s):  
Guinea Pig ◽  
Disease Virus ◽  
Immunosorbent Assay ◽  
Solid Phase ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Specific Detection ◽  
Sandwich Technique ◽  
Mouth Disease Virus ◽  
Foot And Mouth

SUMMARYA solid-phase micro-enzyme-labelled immunosorbent assay (ELISA) using guinea pig antiserum against purified (140S) inactivated foot-and-mouth disease (FMD) virus has been usedin a sandwich technique to specifically measure 140S virus in the presence of 12S material.

scholarly journals Detection of Bovine Antibodies against a Conserved Capsid Epitope as the Basis of a Novel Universal Serological Test for Foot-and-Mouth Disease

2020 ◽  
Vol 58 (6) ◽  
Author(s):  
A. S. Asfor ◽  
N. Howe ◽  
S. Grazioli ◽  
S. Berryman ◽  
K. Parekh ◽  
...  
Keyword(s):  
Large Scale ◽  
Serological Test ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Nonstructural Proteins ◽  
Fmd Virus ◽  
Foot And Mouth ◽  
Serological Surveillance ◽  
The Individual

ABSTRACT Diagnostic tests for foot-and-mouth disease (FMD) include the detection of antibodies against either the viral nonstructural proteins or the capsid. The detection of antibodies against the structural proteins (SP) of the capsid can be used to monitor seroconversion in both infected and vaccinated animals. However, SP tests need to be tailored to the individual FMD virus (FMDV) serotype and their sensitivity may be affected by antigenic variability within each serotype and mismatching between test reagents. As a consequence, FMD reference laboratories are required to maintain multiple type-specific SP assays and reagents. A universal SP test would simplify frontline diagnostics and facilitate large-scale serological surveillance and postvaccination monitoring. In this study, a highly conserved region in the N terminus of FMDV capsid protein VP2 (VP2N) was characterized using a panel of intertype-reactive monoclonal antibodies. This revealed a universal epitope in VP2N which could be used as a peptide antigen to detect FMDV-specific antibodies against all types of the virus. A VP2-peptide enzyme-linked immunosorbent assay (VP2-ELISA) was optimized using experimental and reference antisera from immunized, convalescent, and naïve animals (n = 172). The VP2-ELISA is universal and simple and provided sensitive (99%) and specific (93%) detection of antibodies to all FMDV strains used in this study. We anticipate that this SP test could have utility for serosurveillance during virus incursions in FMD-free countries and as an additional screening tool to assess FMD virus circulation in countries where the disease is endemic.

scholarly journals Enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus: III. Evaluation of antibodies after infection and vaccination

1987 ◽  
Vol 99 (3) ◽  
pp. 733-744 ◽  
Author(s):  
C. Hamblin ◽  
R. P. Kitching ◽  
A. I. Donaldson ◽  
J. R. Crowther ◽  
I. T. R. Barnett
Keyword(s):  
Disease Virus ◽  
Immunosorbent Assay ◽  
Serum Antibody ◽  
Foot And Mouth Disease ◽  
Immunological Response ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Antibody Levels

SUMMARYInvestigations using a liquid-phase blocking sandwich enzyme-linked immunosorbent assay (ELISA) for the measurement of antibodies against foot-and-mouth disease virus (FMDV) in sera from sheep and from cattle are reported, and results compared with those obtained by virus neutralization (VN) tests.Serum antibody titres in sheep after primary vaccination and in cattle challenged with a natural aerosol after vaccination were similar by ELISA and VN. However, the antibody levels detected in sera of cattle during early infection and of vaccinated cattle after intradermolingual challenge were clearly greater by ELISA than by VN.The ELISA titres in cattle sera following synthetic peptide vaccination indicated some relationship to protection and were clearly different from those recorded by VN. On the other hand, the antibody levels following conventional vaccination showed that ELISA and VN titres in cattle sera were related to protection. Although there was a good agreement between the ELISA antibody titre and protection for the four vaccines used, by VN the titre which afforded protection varied depending on the vaccine used.The ELISA was considered therefore to be more reliable than the VN and may prove useful for evaluating the immunological response of animals following infection and following vaccination.

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