scholarly journals Foot-and-Mouth Disease Virus Typing by Complement Fixation and Enzyme-Linked Immunosorbent Assay using Monovalent and Polyvalent Antisera

1992 ◽  
Vol 4 (3) ◽  
pp. 249-253 ◽  
Author(s):  
Albino Alonso ◽  
Mauricio A. Martins ◽  
D. Gomes Maria da Penha ◽  
Rossana Allende ◽  
Magnus S. Söndahl
Keyword(s):  
Cell Culture ◽  
Immunosorbent Assay ◽  
Complement Fixation ◽  
Detection System ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fmd Virus ◽  
Mouth Disease Virus ◽  
Foot And Mouth

An indirect “sandwich” enzyme-linked immunosorbent assay (ELISA) using polyvalent and monovalent antisera was compared with the 50% complement fixation (CF) test for the detection of foot-and-mouth disease (FMD) O, A, and C virus types. ELISA was more sensitive than CF tests when polyvalent antisera were used for detecting the 3 types of virus in epithelial samples, whereas ELISA using monovalent antisera was the least sensitive technique. The ELISA performed with polyvalent antisera was 9 times more sensitive for detecting FMD virus than that with monovalent antisera. However, viral isolation in cell culture was the most sensitive detection system. The combined use of ELISA with polyvalent antisera and cell culture inoculations was the most effective procedure for identifying FMD virus in epithelial samples from the field.

scholarly journals Application of the enzyme linked immunosorbent assay to the detection and identification of foot-and-mouth disease viruses

1979 ◽  
Vol 83 (3) ◽  
pp. 513-519 ◽  
Author(s):  
J. R. Crowther ◽  
E. M. E. Abu Elzein
Keyword(s):  
Tissue Culture ◽  
Immunosorbent Assay ◽  
Complement Fixation ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Detection ◽  
Fmd Virus ◽  
Detection And Identification ◽  
Foot And Mouth

An indirect enzyme linked immunosorbent assay (ELISA) was applied to the detection and identification of foot-and-mouth disease (FMD) virus types. The test proved successful for the specific detection of virus from infected tissue culture, and from epithelial tissues from bovines suspected of having FMD. The ELISA compared favourably with the complement fixation (CF) test, being more sensitive and unaffected by anticomplementary factors.

scholarly journals Enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus: III. Evaluation of antibodies after infection and vaccination

1987 ◽  
Vol 99 (3) ◽  
pp. 733-744 ◽  
Author(s):  
C. Hamblin ◽  
R. P. Kitching ◽  
A. I. Donaldson ◽  
J. R. Crowther ◽  
I. T. R. Barnett
Keyword(s):  
Disease Virus ◽  
Immunosorbent Assay ◽  
Serum Antibody ◽  
Foot And Mouth Disease ◽  
Immunological Response ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Antibody Levels

SUMMARYInvestigations using a liquid-phase blocking sandwich enzyme-linked immunosorbent assay (ELISA) for the measurement of antibodies against foot-and-mouth disease virus (FMDV) in sera from sheep and from cattle are reported, and results compared with those obtained by virus neutralization (VN) tests.Serum antibody titres in sheep after primary vaccination and in cattle challenged with a natural aerosol after vaccination were similar by ELISA and VN. However, the antibody levels detected in sera of cattle during early infection and of vaccinated cattle after intradermolingual challenge were clearly greater by ELISA than by VN.The ELISA titres in cattle sera following synthetic peptide vaccination indicated some relationship to protection and were clearly different from those recorded by VN. On the other hand, the antibody levels following conventional vaccination showed that ELISA and VN titres in cattle sera were related to protection. Although there was a good agreement between the ELISA antibody titre and protection for the four vaccines used, by VN the titre which afforded protection varied depending on the vaccine used.The ELISA was considered therefore to be more reliable than the VN and may prove useful for evaluating the immunological response of animals following infection and following vaccination.

scholarly journals Development of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against the 3B Protein of Foot-and-Mouth Disease Virus

2015 ◽  
Vol 22 (4) ◽  
pp. 389-397 ◽  
Author(s):  
Ming Yang ◽  
Satya Parida ◽  
Tim Salo ◽  
Kate Hole ◽  
Lauro Velazquez-Salinas ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Diagnostic Sensitivity ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Nonstructural Proteins ◽  
Serum Samples ◽  
Mouth Disease Virus ◽  
Foot And Mouth

ABSTRACTFoot-and-mouth disease (FMD) is one of the most highly contagious and economically devastating diseases, and it severely constrains the international trade of animals. Vaccination against FMD is a key element in the control of FMD. However, vaccination of susceptible animals raises critical issues, such as the differentiation of infected animals from vaccinated animals. The current study developed a reliable and rapid test to detect antibodies against the conserved, nonstructural proteins (NSPs) of the FMD virus (FMDV) to distinguish infected animals from vaccinated animals. A monoclonal antibody (MAb) against the FMDV NSP 3B was produced. A competitive enzyme-linked immunosorbent assay (cELISA) for FMDV/NSP antibody detection was developed using a recombinant 3ABC protein as the antigen and the 3B-specific MAb. Sera collected from naive, FMDV experimentally infected, vaccinated carrier, and noncarrier animals were tested using the 3B cELISA. The diagnostic specificity was 99.4% for naive animals (cattle, pigs, and sheep) and 99.7% for vaccinated noncarrier animals. The diagnostic sensitivity was 100% for experimentally inoculated animals and 64% for vaccinated carrier animals. The performance of this 3B cELISA was compared to that of four commercial ELISA kits using a panel of serum samples established by the World Reference Laboratory for FMD at The Pirbright Institute, Pirbright, United Kingdom. The diagnostic sensitivity of the 3B cELISA for the panel of FMDV/NSP-positive bovine serum samples was 94%, which was comparable to or better than that of the commercially available NSP antibody detection kits. This 3B cELISA is a simple, reliable test to detect antibodies against FMDV nonstructural proteins.

scholarly journals Detection and quantification of IgM, IgA, IgG1 and IgG2 antibodies against foot-and-mouth disease virus from bovine sera using an enzyme-linked immunosorbent assay

1981 ◽  
Vol 86 (1) ◽  
pp. 79-85 ◽  
Author(s):  
E. M. E. Abu Elzein ◽  
J. R. Crowther
Keyword(s):  
Serum Proteins ◽  
Solid Phase ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Class ◽  
Fmd Virus ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Preliminary Separation

SUMMARYA simple solid-phase enzyme immunoassay is described for the detection of antibody classes showing activity against foot-and-mouth disease (FMD) virus in bovine sera. The assay achieves a preliminary separation of the specific class of antibody from other serum proteins through immuno-adsorption to class-specific immunoglobulin-coated wells of micro-titre plates. The specific antibody is reacted with FMD virus, which is then detected by an enzyme-labelled anti virus IgG.

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