Growth of Trypanosoma cruzi in vitro: development and application of a continuous-flow culture system

Parasitology ◽  
1982 ◽  
Vol 84 (3) ◽  
pp. 511-526 ◽  
Author(s):  
G. T. Williams ◽  
L. Hudson
Keyword(s):  
Growth Rate ◽  
Trypanosoma Cruzi ◽  
Continuous Flow ◽  
Culture System ◽  
Extreme Values ◽  
Environmental Parameters ◽  
Optimal Growth ◽  
Parasite Growth ◽  
Vitro Development

SummaryThe design and operation of a modular, bacteriological continuous-flow culture system have been adapted for the growth of Trypanosoma cruzi epimastigotes in simple monophasic media. The system was designed to achieve a minimum of 200 days of continuous culture and provision was made for the continuous supply of medium and collection of parasites under sterile conditions. The system provides large quantities of epimastigotes with homogeneous morphology and uniform viability. The system also lends itself tothe analysis of the factors which affect parasite growth. We have examined the effects of changes in environmental parameters on epimastigote growth rate. Optimal growth was observed at 27 °C. The rate ofstirring of the culture had a small but definable effect on the growth rate, which was greatest at 80 r.p.m. Growth was only slightly affected by the level of dissolved oxygen between 10 and 50% saturation, but was inhibited at higher concentrations. Growth was slower at extreme values of pH but showed a broad optimum around pH 7·4.

Effects of carbon monoxide on cardiac muscle cells in culture

1988 ◽  
Vol 255 (3) ◽  
pp. C291-C296 ◽  
Author(s):  
A. C. Nag ◽  
K. C. Chen ◽  
M. Cheng
Keyword(s):  
Growth Rate ◽  
Cardiac Muscle ◽  
Structural Organization ◽  
Optimal Growth ◽  
Muscle Cells ◽  
Cellular Growth ◽  
Cardiac Muscle Cells ◽  
Cells In Culture ◽  
Significant Difference

Embryonic rat cardiac muscle cells grown in the presence of various tensions of CO (5-95%) without the presence of O2 survived and exhibited reduced cell growth, which was concentration dependent. When cardiac muscle cells were grown in the presence of a mixture of CO (10-20%) and O2 (10-20%), the growth rate of these cells was comparable to that of the control cells. Cardiac myocytes continued to beat when exposed to varying tensions of CO, except in the case of 95% CO. The cells exposed to different concentrations of CO contained fewer myofibrils of different stages of differentiation compared with the control and the culture exposed to a mixture of 20% O2 and 20% CO, with cells that contained abundant, highly differentiated myofibrils. There was no significant difference in the structural organization of mitochondria between the control and the surviving experimental cells. It is evident from the present studies that O2 is required for the optimum in vitro cellular growth of cardiac muscle. Furthermore, CO in combination with O2 at a concentration of 10 or 20% can produce optimal growth of cardiac muscle cells in culture.

Effect of morphological integrity, period, and type of culture system on the in vitro development of isolated caprine preantral follicles

2014 ◽  
Vol 82 (2) ◽  
pp. 312-317 ◽  
Author(s):  
A.F.C. Pessoa ◽  
R.M.P. Rocha ◽  
I.R. Brito ◽  
G.M. Silva ◽  
R.N. Chaves ◽  
...  
Keyword(s):  
Culture System ◽  
In Vitro Development ◽  
Preantral Follicles ◽  
Vitro Development

scholarly journals Delineation of precursors in murine spleen that develop in contact with splenic endothelium to give novel dendritic-like cells

Blood ◽  
2010 ◽  
Vol 115 (18) ◽  
pp. 3678-3685 ◽  
Author(s):  
Jonathan K. H. Tan ◽  
Pravin Periasamy ◽  
Helen C. O'Neill
Keyword(s):  
Bone Marrow ◽  
Endothelial Cells ◽  
Culture System ◽  
In Vitro Development ◽  
Cell Lineages ◽  
Mhc Ii ◽  
Murine Spleen ◽  
Vitro Development

Abstract Hematopoietic cell lineages are best described in terms of distinct progenitors with limited differentiative capacity. To distinguish cell lineages, it is necessary to define progenitors and induce their differentiation in vitro. We previously reported in vitro development of immature dendritic-like cells (DCs) in long-term cultures (LTCs) of murine spleen, and in cocultures of spleen or bone marrow (BM) over splenic endothelial cell lines derived from LTCs. Cells produced are phenotypically distinct CD11bhiCD11cloCD8−MHC-II− cells, tentatively named L-DCs. Here we delineate L-DC progenitors as different from known DC progenitors in BM and DC precursors in spleen. The progenitor is contained within the lineage-negative (Lin)−c-kit+ subset in neonatal and adult spleen. This subset has multipotential reconstituting ability in mice. In neonatal spleen, the progenitor is further enriched within the c-kitlo and CD34+ subsets of Lin−c-kit+ cells. These cells seed cocultures of splenic endothelial cells, differentiating to give L-DCs that can activate T cells. L-DC progenitors are distinguishable from described splenic CD11clo DC precursors and from Fms-like tyrosine kinase 3+ DC progenitors in BM. Overall, this study confirms that LTCs are a physiologically relevant culture system for in vitro development of a novel DC type from spleen progenitors.

Phaeotheca dimorphospora sp.nov.: description et caractéristiques culturales

1994 ◽  
Vol 72 (6) ◽  
pp. 808-817 ◽  
Author(s):  
Pierre DesRochers ◽  
G. B. Ouellette
Keyword(s):  
Growth Rate ◽  
Growth Temperature ◽  
Type Species ◽  
Optimal Growth ◽  
Outer Wall ◽  
Dutch Elm Disease ◽  
Optimal Growth Temperature ◽  
Ophiostoma Ulmi ◽  
Mother Cells

An unknown fungus isolated from an elm branch and inhibitory against Ophiostoma ulmi in vitro is described as Phaeotheca dimorphospora sp.nov. This dematiaceous deuteromycete propagates by endoconidia released after exfoliation of chlamydospore outer wall, as in mother cells of the type species Phaeotheca fissurella. However, P. dimorphospora differs from the type species by producing hyaline secondary ameroconidia between the endoconidial masses. Other ameroconidia, similar to the secondary ameroconidia, are produced through the chlamydospore outer wall. The optimal growth temperature of P. dimorphospora is 23 °C, whereas it is 15.5 °C for the type species. On media containing a high dextrose concentration (30 g ∙ L−1), colonies of P. dimorphospora are gray and crustose and grow slowly, at least initially. Conversely, on media with a low dextrose concentration (5 or 10 g ∙ L−1) colonies have a faster growth rate and appear whitish or ivory and fluffy. Key words: Phaeotheca dimorphospora, diagnosis, inhibition, Ophiostoma ulmi, Dutch elm disease.

scholarly journals Simulated Microgravity Using a Rotary Culture System Compromises the In Vitro Development of Mouse Preantral Follicles

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0151062 ◽  
Author(s):  
Shen Zhang ◽  
Dahan Zheng ◽  
Yonggen Wu ◽  
Wei Lin ◽  
Zaichong Chen ◽  
...  
Keyword(s):  
Culture System ◽  
Simulated Microgravity ◽  
In Vitro Development ◽  
Preantral Follicles ◽  
Vitro Development ◽  
Rotary Culture

A continuous flow, perifusion culture system for 8- to 16-cell bovine embryos derived from in vitro culture

1996 ◽  
Vol 46 (8) ◽  
pp. 1441-1450 ◽  
Author(s):  
J.M. Lim ◽  
B.C. Reggio ◽  
R.A. Godke ◽  
W. Hansel
Keyword(s):  
In Vitro Culture ◽  
Continuous Flow ◽  
Culture System ◽  
Bovine Embryos

132 EFFECTS OF OXYGEN TENSION, MEDIUM, AND WELL OF THE WELL ON IN VITRO DEVELOPMENT OF THE MOUSE EMBRYO

2011 ◽  
Vol 23 (1) ◽  
pp. 170
Author(s):  
Y. S. Li ◽  
Z. B. Cao ◽  
Y. Liu ◽  
H. G. Cao ◽  
Y. Tao ◽  
...  
Keyword(s):  
Oxygen Tension ◽  
Culture System ◽  
Total Cell ◽  
Mouse Embryos ◽  
In Vitro Development ◽  
Cell Numbers ◽  
Gas Phases ◽  
Blastocyst Rate ◽  
Vitro Development

The objectives of the present research were to investigate whether embryo culture media have preferences for oxygen tension, to explore the feasibility of using physical lung air to support the in vitro development of mouse embryos, and to evaluate the effect of well of the well (WOW) culture on in vitro preimplantational development of mouse embryos. The results are as follows. First, cleavage rate and blastocyst rate were not significantly different between medium CZB and mKSOM regardless of using 3 gas phases: 4% CO2 + 16% O2 + 78% N2 + 2% H2O (lung air), 5% CO2 + 5% O2 + 90% N2 (5% O2, low oxygen), and 5% CO2 + 95% air (20% O2, high oxygen; P > 0.05), but mean total cell numbers per blastocyst cultured in CZB medium were higher than those in mKSOM when the lung air was used (P < 0.05). Second, when mKSOM was used as the basic medium, the blastocyst rate (22.6%) in the 5% O2 gas phase was notably higher than that in other 2 gas phases (P < 0.05). Third, for the CZB medium, the blastocyst rate was not different significantly among 3 gas environments (P > 0.05). Fourth, both the blastocyst rate (74.6 ± 5.1%) and the mean total cell numbers per blastocyst (76 ± 2) cultured in the WOW system were obviously higher than those in the group culture system (38.2 ± 6.6% and 58 ± 4). Taken together, our results indicate that mKSOM and CZB have preferences for oxygen tension during in vitro culture of mouse embryos, the lung air was reaffirmed to be able to effectively support in vitro preimplantation development of mouse embryos, and the WOW culture system can apparently enhance in vitro developmental competence and blastocyst quality of mouse embryos. L. YS, C. ZB: equal contribution; supported by NSFC (30700574), 863 (2008AA101003).

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