CrypticOnchocercaspecies infecting North American cervids, with implications for the evolutionary history of host associations inOnchocerca

SUMMARYParasites in the genusOnchocercainfect humans, ruminants, camels, horses, suids, and canids, with effects ranging from relatively benign to debilitating. In North America,Onchocerca cervipedisis the sole species known to infect cervids, while at least 5Onchocercaspecies infect Eurasian cervids. In this study, we report the discovery of a cervid-parasitizingOnchocercaonly distantly related toO. cervipedis. To reconstruct the phylogenetic history of the genusOnchocerca, we used newly acquired DNA sequence fromO. cervipedis(from moose in Northwest Territories, Canada) and from the newly discovered species (from white-tailed deer in upstate New York), as well as previously published sequences. Ancestral host reconstructions suggest that host switches have been common throughout the evolutionary history ofOnchocerca, and that bovid- and cervid-parasitizing species have been particularly important sources of descendant species. North America cervids might therefore serve as a source forOnchocercainvasions into new hosts. Given the high density of deer populations, the potential for zoonotic infections may also exist. Our discovery of a newOnchocercaspecies with relatively limited sampling suggests that the diversity ofOnchocercaassociated with cervids in North America may be greater than previously thought, and surveys utilizing molecules and morphology are necessary.