Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi

Parasitology ◽  
1997 ◽  
Vol 115 (6) ◽  
pp. 563-570 ◽  
Author(s):  
J. ARAYA ◽  
M. I. CANO ◽  
H. B. M. GOMES ◽  
E. M. NOVAK ◽  
J. M. REQUENA ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Repetitive Dna ◽  
Copy Number ◽  
Polymorphic Marker ◽  
Genomic Distribution ◽  
Homologous Sequences ◽  
Interspersed Repetitive Dna ◽  
Chromosomal Bands ◽  
Sequence Similarities

We report the molecular characterization of a middle repetitive DNA sequence, named C6, isolated from the Trypanosoma cruzi genome. C6 appears to be a composite repeated element since 3 subregions may be defined within it on the basis of sequence similarities with other T. cruzi genomic sequences. Sequences homologous to C6 are interspersed in the genome and can be mapped out on most chromosomal bands of different T. cruzi strains. The copy number of the C6 element is about 1000 per haploid genome. Given the species specificity and different genomic distribution of C6 homologous sequences among the T. cruzi strains the C6 element could be a useful probe for diagnosis and typing of parasites. C6 is a polymorphic marker with potential as a tool for physical mapping of the T. cruzi genome.

scholarly journals Identification and characterization of domesticated bacterial transposases

2017 ◽  
Author(s):  
Frederic Bertels ◽  
Jenna Gallie ◽  
Paul B Rainey
Keyword(s):  
Host Cell ◽  
Copy Number ◽  
Housekeeping Genes ◽  
Insertion Sequences ◽  
Bacterial Genomes ◽  
Evolutionary Time ◽  
Genomic Distribution ◽  
Selfish Genetic Elements ◽  
Identification And Characterization

ABSTRACTSelfish genetic elements (SGEs), such as insertion sequences (ISs) and transposons are found in most genomes. Transposons are usually identifiable by their high copy number within genomes. In contrast REP associated tyrosine transposases (RAYTs), a recently described class of bacterial transposase, are typically present at just one copypergenome. This suggests that RAYTs no longer copy themselves and thus they no longer function as a typical transposase. Motivated by this possibility we interrogated thousands of fully sequenced bacterial genomes in order to determine patterns of RAYT diversity, their distribution across chromosomes and accessory elements, and rate of duplication. RAYTs encompass exceptional diversity and are divisible into at least five distinct groups. They possess features more similar to housekeeping genes than insertion sequences, are predominantly vertically transmitted and have persisted through evolutionary time to the point where they are now found in 24% of all species for which at least one fully sequenced genome is available. Overall, the genomic distribution of RAYTs suggests that they have been co-opted by host genomes to perform a function that benefits the host cell.

Chromosome localization and characterization of a family of long interspersed repetitive DNA elements from the genus Zea

1995 ◽  
Vol 90 (7-8) ◽  
pp. 1094-1100 ◽  
Author(s):  
R. Aledo ◽  
R. Raz ◽  
A. Monfort ◽  
C. M. Vicient ◽  
P. Puigdomènech ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
Chromosome Localization ◽  
Dna Elements ◽  
Interspersed Repetitive Dna ◽  
Repetitive Dna Elements

Characterization of High-Copy-Number Retrotransposons From the Large Genomes of the Louisiana Iris Species and Their Use as Molecular Markers

Genetics ◽  
2003 ◽  
Vol 164 (2) ◽  
pp. 685-697 ◽  
Author(s):  
Edward K Kentner ◽  
Michael L Arnold ◽  
Susan R Wessler
Keyword(s):  
Molecular Markers ◽  
Copy Number ◽  
High Copy Number ◽  
Marker System ◽  
Transposon Display ◽  
Plant Genomes ◽  
Large Plant ◽  
Backcross Hybrids ◽  
Abundance And Distribution

Abstract The Louisiana iris species Iris brevicaulis and I. fulva are morphologically and karyotypically distinct yet frequently hybridize in nature. A group of high-copy-number TY3/gypsy-like retrotransposons was characterized from these species and used to develop molecular markers that take advantage of the abundance and distribution of these elements in the large iris genome. The copy number of these IRRE elements (for iris retroelement), is ∼1 × 105, accounting for ∼6–10% of the ∼10,000-Mb haploid Louisiana iris genome. IRRE elements are transcriptionally active in I. brevicaulis and I. fulva and their F1 and backcross hybrids. The LTRs of the elements are more variable than the coding domains and can be used to define several distinct IRRE subfamilies. Transposon display or S-SAP markers specific to two of these subfamilies have been developed and are highly polymorphic among wild-collected individuals of each species. As IRRE elements are present in each of 11 iris species tested, the marker system has the potential to provide valuable comparative data on the dynamics of retrotransposition in large plant genomes.

Characterization of repetitive DNA in rye (Secale cereale)

Chromosoma ◽  
1974 ◽  
Vol 48 (4) ◽  
pp. 427-440 ◽  
Author(s):  
P. K. Ranjekar ◽  
J. G. Lafontaine ◽  
D. Pallotta
Keyword(s):  
Repetitive Dna ◽  
Secale Cereale
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