Single-particle reconstruction of biological macromolecules in electron microscopy – 30 years
2009 ◽
Vol 42
(3)
◽
pp. 139-158
◽
Keyword(s):
AbstractThis essay gives the autho's personal account on the development of concepts underlying single-particle reconstruction, a technique in electron microscopy of macromolecular assemblies with a remarkable record of achievements as of late. The ribosome proved to be an ideal testing ground for the development of specimen preparation methods, cryo-EM techniques, and algorithms, with discoveries along the way as a rich reward. Increasingly, cryo-EM and single-particle reconstruction, in combination with classification techniques, is revealing dynamic information on functional molecular machines uninhibited by molecular contacts.
2015 ◽
Vol 21
(4)
◽
pp. 876-885
◽