XXII.—Deficiency Effects of Ultra-violet Light in Drosophila melanogaster

1942 ◽  
Vol 61 (3) ◽  
pp. 297-315
Author(s):  
B. M. Slizynski

Stadler and Sprague in a series of papers (1936) succeeded in demonstrating by a genetical method the effect of ultra-violet radiation in maize pollen, and found that in their experiments in the X-ray series the chromosomal changes are very common, while in the ultra-violet series they are rare.

1936 ◽  
Vol 9 (4) ◽  
pp. 570-572 ◽  
Author(s):  
Kurt H. Meyer ◽  
Cesare Ferri

Abstract The action of ultra-violet radiation on rubber has been the object of a long series of investigations. According to van Rossem, rubber is depolymerized under the action of light. Asano on the other hand thinks that ultra-violet light, is able to bring about either polymerization or depolymerization according to its wave-length. More recently Dogadkin and Pantschenkov have carried out experiments in an atmosphere of nitrogen, during the course of which they have found a strong diminution in the viscosity. From this fact they have concluded that light is able to cause depolymerization and micellar degradation. We have undertaken a study of the action of ultra-violet light on rubber in order to prove whether the double cis-linkages of rubber undergo a transposition into trans-linkages, for numerous instances are known where light causes these cis-trans-transpositions. In the case of rubber, one should obtain, therefore, either a hydrocarbon of the gutta-percha type or, if light causes a sort of cis-trans-equilibrium, a hydrocarbon with double cis-linkages distributed irregularly. In our experiments we were extremely careful to exclude oxygen, since some years ago Henri proved that ultra-violet light activates greatly the oxidation of rubber. On the other hand it is known that oxidation causes a diminution in the length of the chains which modifies considerably the physical properties, for example, the viscosity, and which may mask the effect produced by light.


The alkaline earth azides decompose at appreciable rates at temperatures above 100° C, and the reactions which occur are of the accelerating type increasing in speed as the 6th-8th power of the time, there being a marked induction period during which the reaction occurs at a negligible rate (Harvey 1933; Marke 1937; Maggs 1939). The decomposition is accelerated and the length of the induction period shortened when the solid is acted upon by β -rays at room temperature before the heat treatment (Garner and Moon 1933; Maggs 1939). It was, therefore, possible that ultra-violet light would cause an acceleration of these reactions in a similar manner. Muller and Brous (1933) have shown that sodium azide is decomposed by ultra-violet light at a rate which is proportional to the intensity and that the threshold wave-length for the decomposition is around 405 m µ . In the present investigation it is shown that barium and strontium azides are decomposed by ultra-violet light at room temperature and that the thermal decomposition of these substances is accelerated by pre-treatment with this radiation.


1935 ◽  
Vol 18 (3) ◽  
pp. 351-355 ◽  
Author(s):  
R. H. Oster ◽  
W. A. Arnold

Data obtained on yeast irradiated with monochromatic ultra-violet radiation has been analyzed for the number of quantum hits involved in the production of different degrees of inhibition of cell division, according to the method proposed by Mme. Curie (1929). Sufficient data are not available for a rigorous determination, but the calculated results tend to indicate that a multiple hit to kill relation is followed, that different numbers of hits are involved in the production of different degrees of inhibition, and that this number increases with increase in the degree of damage sustained.


In an attempt to fill up the gap between the shortest ultra-violet light waves hitherto produced and the longest X-ray waves known, Hughes recently made a study of the characteristic X-rays emitted by carbon and by boron when bombarded by electrons. In this investigation the energy of the bombarding electrons was increased by steps, and the critical values were determined that were necessary and just sufficient to cause the bombarded element to emit its characteristic radiations with measurable intensities. These characteristic radiations were detected, and their intensities measured, by their photo-electric action on an insulated electrode of nickel or of silver. The method followed by Hughes in recording his results was to plot curves with the values of the accelerating potentials of the electrons as abscissæ and the measures of the photo-electric effect divided by the corresponding electronic currents as ordinates. At certain critical accelerating voltages it was found that these curves showed marked and abrupt kinks or changes of curvature, and these changes were taken to connote the beginning of the emission by the bombarded element of its characteristic radiations.


1934 ◽  
Vol 18 (2) ◽  
pp. 265-278 ◽  
Author(s):  
Frederick L. Gates

Determination of the absorption spectra of pure preparations of Northrop's crystalline pepsin inactivated by irradiation with ultra-violet light shows that the total absorption in the ultra-violet region of the spectrum increases with the degree of inactivation. This increase is especially marked between 2400 and 2750 Å.u. The rate of photoinactivation is shown to be sensitive to changes in pH, increasing with lower values, and evidently bears a one-quantum relationship to the energy flux. Tests of the rate of inactivation of pepsin exposed to several different bands of the ultra-violet spectrum, in relation to the absorbed energy, indicate that the destruction spectrum of the enzyme agrees essentially with its absorption spectrum and is similar to that of urease.


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