Improved Recognition and Quantitation of Oncornaviruses Using Modified Pointed Beem Capsules

1974 ◽  
Vol 32 ◽  
pp. 112-113
Author(s):  
R. Stephens ◽  
K. Traul ◽  
G. Lowry ◽  
D. Woolf ◽  
J. Lelek
Keyword(s):  
Staining Technique ◽  
Negative Staining ◽  
Cell Debris ◽  
Embedding Method ◽  
Particle Counting ◽  
Viral Particles ◽  
Particle Recognition ◽  
Improve Accuracy ◽  
Mycoplasmal Contamination

Since the review on virus quantitation by Sharp (1965), interest has increased in developing more accurate methods to recognize and quantitate oncornaviruses. The available quantitation procedures (Sharp 1949, Williams and Backus 1949, Kellenberger and Arber 1957), including the negative staining technique introduced by Monroe and Brandt (1970), present problems of virus recognition, especially under the following conditions: 1. highly pleomorphic particles; 2. low numbers of viral particles; 3. mycoplasmal contamination and 4. large amounts of cell debris. To improve accuracy in particle counting and particle recognition, an embedding method using pointed Beem capsules was developed.

A Rapid Method for Viral Particle Detection in Viral-Induced Gastroenteritis: A TEM Study

1995 ◽  
Vol 1 (5) ◽  
pp. 185-189
Author(s):  
M. John Hicks ◽  
James P. Barrish ◽  
Elizabeth S. Hayes ◽  
Laurie C. Leer ◽  
Mary K. Estes ◽  
...  
Keyword(s):  
Staining Method ◽  
Pediatric Population ◽  
Staining Technique ◽  
Negative Staining ◽  
Viral Gastroenteritis ◽  
Particle Detection ◽  
Fecal Samples ◽  
Infectious Gastroenteritis ◽  
Viral Particles ◽  
Tem Method

Infectious gastroenteritis is a common cause of hospitalization in the pediatric population. The most frequent cause of gastroenteritis is viral in origin. The purpose of this study was to compare a rapid modified negative-staining TEM method with the conventional pseudoreplica technique in detection of viral particles in fecal samples from children with viral gastroenteritis. The modified negative-staining method resulted in a significantly higher (2.5 ± 0.5, p = 0.02) viral rating score than that for the conventional pseudoreplica technique (1.7 ± 0.4). In addition, the preparation time for the negative-staining method was approximately one fifth that for the conventional pseudoreplica technique. Rapid diagnosis of viral gastroenteritis may be made by ultrastructural detection of viral particles in fecal samples using the negative staining technique.

The fine structure and the protein composition of γ phage of Bacillus anthracis

1975 ◽  
Vol 21 (11) ◽  
pp. 1889-1892 ◽  
Author(s):  
Takashi Watanabe ◽  
Akinori Morimoto ◽  
Toshiro Shiomi
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Bacillus Anthracis ◽  
Protein Composition ◽  
Staining Technique ◽  
Negative Staining ◽  
Polyacrylamide Gels ◽  
Long Tail

The fine structure of γ phage of Bacillus anthracis was studied by electron microscopy with a negative-staining technique. The phage has a hexagonal head and a long tail without a sheath. By electrophoresis on polyacrylamide gels, the proteins of the phage particles are separate into 10 polypeptides with moleclar weights ranging from 140 000 to 12 000.

Structure of a Marine Bacteriophage as Revealed by the Negative-Staining Technique

1966 ◽  
Vol 91 (2) ◽  
pp. 819-822 ◽  
Author(s):  
Artrice F. Valentine ◽  
Peter K. Chen ◽  
Rita R. Colwell ◽  
George B. Chapman
Keyword(s):  
Staining Technique ◽  
Negative Staining

Lipid Infusions in Man

1973 ◽  
Vol 29 (02) ◽  
pp. 450-460 ◽  
Author(s):  
T Hovig ◽  
K. A Grøttum
Keyword(s):  
Electron Microscopy ◽  
Lipid Emulsion ◽  
Staining Technique ◽  
Negative Staining ◽  
Scanning Electron Micrographs ◽  
Lipid Particles ◽  
Ultrathin Sections ◽  
Small Platelet ◽  
Scanning Electron ◽  
Platelet Shape

SummaryThe soybean lipid emulsion “Intralipid” was infused in 10 volunteer individuals, and platelets, obtained up to 22 hours after the infusions, were studied by transmission and scanning electron microscopy. Using negative staining technique, platelet coating with lipid particles could be demonstrated. Platelet engulf ment of the lipid particles was found in ultrathin sections. The lipid particles appeared to enter the platelets via invaginations of the surface membranes and were located in vacuoles and in’ ‘humps”. The platelet shape and internal structure was strikingly well preserved and no evidence of platelet aggregation was demonstrated. Small platelet cytoplasmic fragments containing lipid were observed, and it is suggested that these may be pinched off from the platelets. Both with negative staining and in scanning electron micrographs occasional clumps of lipid particles were observed, and the possibility can not be excluded that such clumps may interfere with microcirculatory flow.

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