Lipid Infusions in Man

1973 ◽  
Vol 29 (02) ◽  
pp. 450-460 ◽  
Author(s):  
T Hovig ◽  
K. A Grøttum
Keyword(s):  
Electron Microscopy ◽  
Lipid Emulsion ◽  
Staining Technique ◽  
Negative Staining ◽  
Scanning Electron Micrographs ◽  
Lipid Particles ◽  
Ultrathin Sections ◽  
Small Platelet ◽  
Scanning Electron ◽  
Platelet Shape

SummaryThe soybean lipid emulsion “Intralipid” was infused in 10 volunteer individuals, and platelets, obtained up to 22 hours after the infusions, were studied by transmission and scanning electron microscopy. Using negative staining technique, platelet coating with lipid particles could be demonstrated. Platelet engulf ment of the lipid particles was found in ultrathin sections. The lipid particles appeared to enter the platelets via invaginations of the surface membranes and were located in vacuoles and in’ ‘humps”. The platelet shape and internal structure was strikingly well preserved and no evidence of platelet aggregation was demonstrated. Small platelet cytoplasmic fragments containing lipid were observed, and it is suggested that these may be pinched off from the platelets. Both with negative staining and in scanning electron micrographs occasional clumps of lipid particles were observed, and the possibility can not be excluded that such clumps may interfere with microcirculatory flow.

Use of electron microscopy to characterize the surfaces of flocculent and nonflocculent yeast cells

1989 ◽  
Vol 35 (12) ◽  
pp. 1081-1086 ◽  
Author(s):  
Byron F. Johnson ◽  
L. C. Sowden ◽  
Teena Walker ◽  
Bong Y. Yoo ◽  
Gode B. Calleja
Keyword(s):  
Electron Microscopy ◽  
Fission Yeast ◽  
Budding Yeast ◽  
The Other ◽  
Yeast Cells ◽  
Scanning Electron Micrographs ◽  
Soft Or ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Scanning Electron

The surfaces of flocculent and nonflocculent yeast cells have been examined by electron microscopy. Nonextractive preparative procedures for scanning electron microscopy allow comparison in which sharp or softened images of surface details (scars, etc.) are the criteria for relative abundance of flocculum material. Asexually flocculent budding-yeast cells cannot be distinguished from nonflocculent budding-yeast cells in scanning electron micrographs because the scar details of both are well resolved, being hard and sharp. On the other hand, flocculent fission-yeast cells are readily distinguished from nonflocculent cells because fission scars are mostly soft or obscured on flocculent cells, but sharp on nonflocculent cells. Sexually and asexually flocculent fission-yeast cells cannot be distinguished from one another as both are heavily clad in "mucilaginous" or "hairy" coverings. Examination of lightly extracted and heavily extracted flocculent fission-yeast cells by transmission electron microscopy provides micrographs consistent with the scanning electron micrographs.Key words: flocculation, budding yeast, fission yeast, scanning, transmission.

The fine structure and the protein composition of γ phage of Bacillus anthracis

1975 ◽  
Vol 21 (11) ◽  
pp. 1889-1892 ◽  
Author(s):  
Takashi Watanabe ◽  
Akinori Morimoto ◽  
Toshiro Shiomi
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Bacillus Anthracis ◽  
Protein Composition ◽  
Staining Technique ◽  
Negative Staining ◽  
Polyacrylamide Gels ◽  
Long Tail

The fine structure of γ phage of Bacillus anthracis was studied by electron microscopy with a negative-staining technique. The phage has a hexagonal head and a long tail without a sheath. By electrophoresis on polyacrylamide gels, the proteins of the phage particles are separate into 10 polypeptides with moleclar weights ranging from 140 000 to 12 000.

Alternative Method of Rapid Drying Vascular Specimens for Scanning Electron Microscopy

2003 ◽  
Vol 10 (2) ◽  
pp. 285-287 ◽  
Author(s):  
DáŠa Slížová ◽  
Otakar Krs ◽  
Blanka PospíŠilová
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Micrographs ◽  
Low Cost ◽  
High Rate ◽  
Drying Methods ◽  
Scanning Electron Micrographs ◽  
Air Drying ◽  
Critical Point Drying ◽  
Scanning Electron

Purpose: To report the use of hexamethyldisilazane (HMDS) as an alternative to critical point drying for preparing stented canine peripheral vessels for scanning electron microscopy (SEM). Technique: Vascular specimens were fixed in 4% formaldehyde overnight, dehydrated in a graded ethanol series, followed by immersion in 100% hexamethyldisilazane. After air drying, the specimens were mounted on stainless steel stubs, coated with gold, and examined in the SEM. The electron micrographs were of high quality, showing the layers of the vascular wall and the incorporated stent covered by a neointimal layer. The micrographs were comparable to corresponding histological sections, but detailed endothelial patterns were more visible. Conclusions: HMDS treatment and subsequent air drying provides good quality scanning electron micrographs that reveal both endothelial patterns and the layered architecture of stented vessels. The disadvantage of HMDS drying may be a shrinkage and distortion similar to other drying agents. Ease of handling, low cost, and a high rate of success are advantages that favor HMDS desiccation over other drying methods.

Delicate Botanical Specimens Preserved for Scanning Electron Microscopy by Critical Point Drying

1971 ◽  
Vol 29 ◽  
pp. 450-451
Author(s):  
Arthur L. Cohen ◽  
Gerald E. Garner
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Critical Point ◽  
Freeze Drying ◽  
Animal Cell ◽  
Pollen Grains ◽  
Scanning Electron Micrographs ◽  
Animal Cells ◽  
Critical Point Drying ◽  
Scanning Electron

The surface forms and structures of animal cells have been strikingly preserved for scanning electron microscopy by freeze-drying and by critical point drying both by the method with CO2 used as the transitional fluid and the later procedure which uses a fluorocarbon (Freon 13) as a medium for the transition from the liquid to the gaseous environment. Freeze-drying is often prolonged (5-12 hours as compared with an hour or less by the critical point method) and in our experience with mold cultures on agar, the substrate shrivels and cracks and hyphal filaments are distorted.Despite, and possibly because of a flexible but inelastic cell wall, plant cells often show greater distortion than do animal cells after evaporative drying or replacement dehydration for mixrotechnical work. The animal cell membrane can contract more or less uniformly on drying - as shown by the numerous micrographs of well-preserved erythrocytes, while plant cell walls often crumple. The many scanning electron micrographs of partially collapsed pollen grains bear witness to this fact.

Quantitative Scanning Electron Microscopy of the Cellular Structure of Wood

1990 ◽  
Vol 48 (1) ◽  
pp. 554-555
Author(s):  
Barbara A. Reine ◽  
Norman J. Fowler ◽  
R. M. Fisher
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Shear Strength ◽  
Cellular Structure ◽  
Cell Structure ◽  
Cork Oak ◽  
Scanning Electron Micrographs ◽  
Quantitative Interpretation ◽  
Structural Components ◽  
Scanning Electron

The physical properties of wood, such as density, moisture content, tensile, compression and shear strength, elastic modulus and anisotropy all vary considerably between different species of wood. These variations are reflected in the long standing differentiations into hard and soft woods that have been established as well as quality classifications or commercial grades such as clear grain, select, structural etc. that are commonly used. These variations in properties arise from differences in the size, shape and distribution of the cells, as well as in the thickness of the cellulose-lignin walls that enclose them.Scanning electron micrographs that are representative of the variations in cellular structure that exist between woods are shown in Figure 1 for ultrasoft hard maple and ultrasoft cork oak. These higher magnification images illustrate the duplex cell configuration that is required to sustain the growth of trees and support their height. Although the differences in cell structure are quite apparent in the micrographs, any quantitative interpretation of the various properties mentioned above requires detailed measurements of specific microstructural components to obtain statistically significant measurements of the relevant structural components.

scholarly journals Study on biofilm-forming properties of clinical isolates of Staphylococcus aureus

2011 ◽  
Vol 6 (05) ◽  
pp. 403-409 ◽  
Author(s):  
Yasmeen Taj ◽  
Farhan Essa ◽  
Faisal Aziz ◽  
Shahana Urooj Kazmi
Keyword(s):  
Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Assay Method ◽  
Scanning Electron Micrographs ◽  
Clinical Specimens ◽  
Biofilm Production ◽  
Important Virulence Factor ◽  
Air Liquid Interface ◽  
Scanning Electron

Introduction: The purpose of this study was to observe the formation of biofilm, an important virulence factor, by isolates of Staphylococcus aureus (S. aureus) in Pakistan by different conventional methods and through electron microscopy. Methodology: We screened 115 strains of S. aureus isolated from different clinical specimens by tube method (TM), air-liquid interface coverslip assay method, Congo red agar (CRA) method, and scanning electron microscopy (SEM). Results: Out of 115 S. aureus isolates, 63 (54.78%) showed biofilm formation by tube method. Biofilm forming bacteria were further categorized as high producers (n = 23, 20%) and moderate producers (n = 40, 34.78%). TM coordinated well with the coverslip assay for strong biofilm-producing strains in 19 (16.5%) isolates. By coverslip method, weak producers were difficult to differentiate from biofilm negative isolates. Screening on CRA showed biofilm formation only in four (3.47%) strains. Scanning electron micrographs showed the biofilm-forming strains of S. aureus arranged in a matrix on the propylene surface and correlated well with the TM. Conclusion: Biofilm production is a marker of virulence for clinically relevant staphylococcal infections. It can be studied by various methods but screening on CRA is not recommended for investigation of biofilm formation in Staphylococcus aureus. Electron micrograph images correlate well with the biofilm production as observed by TM. 

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