Image Reconstruction Applied to High Voltage Microscopy

1974 ◽  
Vol 32 ◽  
pp. 396-397
Author(s):  
D. E. Johnson ◽  
J. Pfeifer
Keyword(s):  
Electron Microscopy ◽  
Image Reconstruction ◽  
High Voltage ◽  
Three Dimensional ◽  
Two Dimensional ◽  
Three Dimensional Reconstruction ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron ◽  
Dimensional Reconstruction ◽  
Quantitative Results

Greatly increased specimen penetration, which is the principle advantage of high voltage electron microscopy, carries with it an increased need for techniques to interpret the large amount of three-dimensional information projected into two-dimensional micrographs. Stereo views can provide very useful information and are widely used. However, for the general specimen, stereo views are limited in their ability to produce quantitative results. At the high voltage microscope facility, Univ. of Wisconsin, we have begun a program to develop and apply three dimensional reconstruction techniques to the microscopy of thick specimens.

Ultrastructural three-dimensional reconstruction of a T lymphocyte

1992 ◽  
Vol 50 (1) ◽  
pp. 592-593
Author(s):  
Karen K. Bovard ◽  
Joseph N. Marcus
Keyword(s):  
Electron Microscopy ◽  
T Lymphocyte ◽  
High Voltage ◽  
Three Dimensional ◽  
High Voltage Electron Microscopy ◽  
Analysis Techniques ◽  
Nucleolar Organizing Regions ◽  
Voltage Electron ◽  
Dimensional Reconstruction ◽  
Limited Depth

There have been many electron microscopy studies of normal and neoplastic lymphocyte microanatomy. However, only a few use three-dimensional analysis techniques to visualize organelle relationships, such as nuclear shape and chromatin distribution, nucleolar organizing regions, and, by high voltage electron microscopy, the microtubular networks over a limited depth though the cell. To date, there has been no three-dimensional serial reconstuction of a T Lymphocyte and its organelles.

Three-Dimensional Visualization of the T-System of Frog Muscle Using High Voltage Electron Microscopy and a Lanthanum Stain

1977 ◽  
Vol 35 ◽  
pp. 570-571 ◽  
Author(s):  
Lee D. Peachey ◽  
Clara Franzini-Armstrong
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Three Dimensional ◽  
Biological Tissues ◽  
High Voltage Electron Microscopy ◽  
Transverse Tubular System ◽  
Peroxidase Reaction ◽  
Voltage Electron ◽  
High Voltage Electron ◽  
T System

The effective study of biological tissues in thick slices of embedded material by high voltage electron microscopy (HVEM) requires highly selective staining of those structures to be visualized so that they are not hidden or obscured by other structures in the image. A tilt pair of micrographs with subsequent stereoscopic viewing can be an important aid in three-dimensional visualization of these images, once an appropriate stain has been found. The peroxidase reaction has been used for this purpose in visualizing the T-system (transverse tubular system) of frog skeletal muscle by HVEM (1). We have found infiltration with lanthanum hydroxide to be particularly useful for three-dimensional visualization of certain aspects of the structure of the T- system in skeletal muscles of the frog. Specifically, lanthanum more completely fills the lumen of the tubules and is denser than the peroxidase reaction product.

High-voltage electron microscopy of maxillary gland of spirochete-infected brine shrimp: lesion of efferent tubule

1988 ◽  
Vol 46 ◽  
pp. 362-363
Author(s):  
G. E. Tyson ◽  
M. J. Song
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Brine Shrimp ◽  
Natural Populations ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron ◽  
High Voltage Electron ◽  
Infected Adult

Natural populations of the brine shrimp, Artemia, may possess spirochete- infected animals in low numbers. The ultrastructure of Artemia's spirochete has been described by conventional transmission electron microscopy. In infected shrimp, spirochetal cells were abundant in the blood and also occurred intra- and extracellularly in the three organs examined, i.e. the maxillary gland (segmental excretory organ), the integument, and certain muscles The efferent-tubule region of the maxillary gland possessed a distinctive lesion comprised of a group of spirochetes, together with numerous small vesicles, situated in a cave-like indentation of the base of the tubule epithelium. in some instances the basal lamina at a lesion site was clearly discontinuous. High-voltage electron microscopy has now been used to study lesions of the efferent tubule, with the aim of understanding better their three-dimensional structure.Tissue from one maxillary gland of an infected, adult, female brine shrimp was used for HVEM study.

scholarly journals Three-Dimensional Imaging of a Long-Period Stacking Ordered Phase in Mg97Zn1Gd2 Using High-Voltage Electron Microscopy

2016 ◽  
Vol 57 (6) ◽  
pp. 918-921 ◽  
Author(s):  
Kazuhisa Sato ◽  
Shunya Tashiro ◽  
Yohei Yamaguchi ◽  
Takanori Kiguchi ◽  
Toyohiko J. Konno ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
High Voltage Electron Microscopy ◽  
Long Period ◽  
Voltage Electron ◽  
High Voltage Electron
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