Herpestype Virus and Type C Particles in Spontaneous Equine Lymphoma

1970 ◽  
Vol 28 ◽  
pp. 162-163
Author(s):  
K. Maruyama ◽  
G. R. Swearingen ◽  
L. Dmochowski ◽  
J. H. Jardine ◽  
G. N. Joiner
Keyword(s):  
Lymph Node ◽  
Cultured Cells ◽  
Reticulum Cell ◽  
Cell Type ◽  
Virus Particles ◽  
Lymph Node Cells ◽  
Type C ◽  
Indented Nucleus ◽  
Nuclear Protrusion

Herpestype and type C virus particles are known to produce tumors in animals of certain species, and to be present in a variety of human tissues. Similar particles were found in a horse lymphoma.Electron microscope study was carried out on a tumorous prescapular lymph node from an adult female horse with spontaneous lymphoma. Lymph node cells grown in vitro for a total of 105 days (6th passage) were also studied. The lymph node was infiltrated by blastic cells of reticulum cell type and of lymphoblast type. No virus particles were found in the original lymph node. The cultured cells have a large cytoplasm with well developed rough endoplasmic reticulum and Golgi's apparatus, and an indented nucleus with marginated chromatin. Occasionally, intranuclear fibrous structures were observed. Elongated nuclear protrusion were frequently seen.

Viropexis and Phagocytosis of Type B (Mouse Mammary Tumor) Virus Particles in vivo

1973 ◽  
Vol 31 ◽  
pp. 580-581
Author(s):  
Gabriel Seman ◽  
Leon Dmochowski
Keyword(s):  
Tumor Cells ◽  
Type A ◽  
Reticulum Cell ◽  
Type B ◽  
Cytoplasmic Matrix ◽  
Virus Particles ◽  
Tumor Virus ◽  
Type C

Electron microscope examination of spleen tumors of RIII/Dm female mice with reticulum cell sarcoma revealed the presence of extracellular and budding type C, type B, and intracytoplasmic type A virus particles. Penetration of type B particles into cytoplasm of tumor cells has been observed. It consisted of inclusion of the particles into pinocytotic vesicles and of the release of the nucleoids into the cytoplasmic matrix. They were frequently seen penetrating into tumor cells producing type C or intracytoplasmic type A particles at the same time (Figs. 1 and 2). A similar penetration of other viruses into cells in vitro has been described as viropexis.

Ultrastructural Correlations between Clonal Human Tumor Colonies and in Vivo Neoplasms

1982 ◽  
Vol 40 ◽  
pp. 210-211
Author(s):  
M.J. Murphy ◽  
R.R. Price ◽  
J.C. Sloman
Keyword(s):  
Cultured Cells ◽  
Human Tumor ◽  
Cell Type ◽  
Tumor Mass ◽  
Initial Cell ◽  
Cloning Assay ◽  
Human Tumor Cloning ◽  
The Relationship

The in vitro human tumor cloning assay originally described by Salmon and Hamburger has been applied recently to the investigation of differential anti-tumor drug sensitivities over a broad range of human neoplasms. A major problem in the acceptance of this technique has been the question of the relationship between the cultured cells and the original patient tumor, i.e., whether the colonies that develop derive from the neoplasm or from some other cell type within the initial cell population. A study of the ultrastructural morphology of the cultured cells vs. patient tumor has therefore been undertaken to resolve this question. Direct correlation was assured by division of a common tumor mass at surgical resection, one biopsy being fixed for TEM studies, the second being rapidly transported to the laboratory for culture.

Effect of Interleukin-l?? on the In Vitro Activation of Tumor-Draining Lymph Node Cells for Adoptive Immunotherapy

1994 ◽  
Vol 16 (1) ◽  
pp. 1-12
Author(s):  
James M. Hammel ◽  
Melissa K. Tuck ◽  
Jon M. Hain ◽  
Alfred E. Chang ◽  
Vernon K. Sondak
Keyword(s):  
Lymph Node ◽  
Adoptive Immunotherapy ◽  
In Vitro Activation ◽  
Lymph Node Cells ◽  
Draining Lymph Node ◽  
Tumor Draining Lymph Node

scholarly journals Properties of reticulum cell sarcomas in SJL/J mice. V. Nature of reticulum cell sarcoma surface antigen which induces proliferation of normal SJL/J T cells.

1977 ◽  
Vol 146 (1) ◽  
pp. 132-145 ◽  
Author(s):  
N M Ponzio ◽  
C S David ◽  
D C Shreffler ◽  
G J Thorbecke
Keyword(s):  
T Cells ◽  
Lymph Node ◽  
T Lymphocytes ◽  
Tumor Cells ◽  
Proliferative Response ◽  
Reticulum Cell ◽  
Reticulum Cell Sarcoma ◽  
Cell Surface Antigens ◽  
Cell Sarcoma ◽  
Lymph Node Cells

The results of studies on the reticulum cell sarcoma (RCS) tumors of SJL/J mice presented here, indicate that spontaneous tumors, which arise in older mice, also possess the capacity to induce the vigorous proliferative response in syngenetic T lymphocytes that are characteristic of the transplantable RCS lines. Analysis of cell surface antigens revealed the presence of Ia determinats on gradient-purified transplantable RCS tumor cells; however, these cells did not express Thy 1.2, nIg, or, any of the viral proteins that were tested for by specific antisera. Pretreatment of RCS cells with anti-Ia sera and complement-deleted cells that were stimulatory for syngenetic T lymphocytes, and addition of anti-Ia sera directly to cultures blocked the proliferative response at the stimulator (RCS) cell level. Lymph node cells from H-2(8) strains other than SJL/J, including A.SW and B10.S also gave proliferative responses to RCS cells, although lower in magnitude. A requirement on the part of responding cells for identity with RCS cells at the Ir region was indicated by the finding that A.TH but not A.TL lymph node cells responded to RCS. It is concluded that RCS cells stimulate Ir-region identical T cells (without evidence of presensitization) through a modification in the expression of Ia antigens on the surface of the tumor cells.

scholarly journals CYTOTOXICITY MEDIATED BY SOLUBLE ANTIGEN AND LYMPHOCYTES IN DELAYED HYPERSENSITIVITY

1968 ◽  
Vol 128 (6) ◽  
pp. 1255-1265 ◽  
Author(s):  
Nancy H. Ruddle ◽  
Byron H. Waksman
Keyword(s):  
Lymph Node ◽  
Cytotoxic Effect ◽  
Specific Antigen ◽  
Delayed Hypersensitivity ◽  
Soluble Antigen ◽  
Heterologous Proteins ◽  
Rat Embryo ◽  
Protein Carrier ◽  
Lymph Node Cells

Damage of rat embryo fibroblasts in the presence of sensitized lymph node cells reacting with specific antigen was shown to be closely correlated with delayed hypersensitivity in the animals from which the lymph node cells were taken. The phenomenon was not correlated with Arthus reactivity. In. animals sensitized with picryl conjugates of ovalbumin or human serum albumin, skin reactivity and the in vitro cytotoxic effect could be elicited only with the homologous conjugate or the protein carrier alone and not with picryl conjugates of heterologous proteins. Lewis rats developed more intense delayed sensitivity than BN rats, and Lewis lymph node cells were correspondingly more effective in producing specific damage of both syngeneic and allogeneic fibroblasts.

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