A Simple Dichromatic Stain for Plastic Embedded Tissues

1970 ◽  
Vol 28 ◽  
pp. 296-297 ◽  
Author(s):  
G. R. Mackay ◽  
M. L. Mead
Keyword(s):  
Electron Microscopy ◽  
Toluidine Blue ◽  
Biological Material ◽  
Good Reproducibility ◽  
Routine Work ◽  
Staining Techniques

Color contrasting of 1 to 2 micron sections of plastic embedded biological material is an important adjunct to electron microscopy. The procedures in general use today are simple and rapid giving monochromatic results, e.g., toluidine blue. Although many di- and polychromatic histologic staining techniques have been modified to obtain a counterstaining effect with plasticembedded tissue, the methods are usually undesirable for routine work because they are time consuming, complicated and often defy good reproducibility.

Proteoglycan alterations in rheumatoid arthritis—a light and electron microscopy study

1978 ◽  
Vol 36 (2) ◽  
pp. 676-677
Author(s):  
Nelson S. Mitchell
Keyword(s):  
Rheumatoid Arthritis ◽  
Electron Microscopy ◽  
Toluidine Blue ◽  
Light And Electron Microscopy ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Clear Space ◽  
Staining Techniques ◽  
Femoral Condyles ◽  
Control Samples

We have previously studied the ultramorphology oh cells in rheumatoid arthritis using conventional staining techniques. We have recently reported a technique for the precipitation and fixation of proteoglycan using Toluidine Blue 0 or Safranin 0 which permits simultaneous localization of proteoglycan using either light or electron microscopy in sections cut from the same block. This paper reports a study of rheumatoid arthritic cartilage using this technique.Samples oh cartilage were removed from the femoral condyles of thirteen patients with classical rheumatoid arthritis who were having operations on the knee. Control samples were removed from the knees of eleven normal patients who were having surgery for recent trauma. In both Instances, the tissues were prepared with Toluidine Blue or Safranin 0 Introduced into the fixation process as will be described.When fixed and stained by conventional methods, articular cartilage has been traditionally reported to consist ofi chondrocytes lying in a matrix of collagen and proteoglycan though separated from this matrix by a pericellular clear space, “halo” or “lacuna” of varying dimensions. This pericellular space was thought to contain only small amounts of collagen.

Effect of ADH on Rat Renal Papilla, an Ultrastructural and Cytochemical Study

1973 ◽  
Vol 31 ◽  
pp. 410-411
Author(s):  
C. N. Sun ◽  
H. J. White ◽  
E. J. Towbin
Keyword(s):  
Electron Microscopy ◽  
Diabetes Insipidus ◽  
Renal Papilla ◽  
Milk Diet ◽  
Intercellular Spaces ◽  
Cytochemical Study ◽  
Collecting Tubule ◽  
Concentrate Urine ◽  
Staining Techniques ◽  
Collecting Tubules

Diabetes insipidus and compulsive water drinking are representative of two categories of antidiuretic hormone (ADH) lack. We studied a strain of rats with congenital diabetes insipidus homozygote (DI) and normal rats on an isocaloric fortified dilute milk diet. In both cases, the collecting tubules could not concentrate urine. Special staining techniques, Alcian Blue-PAS for light microscopy and lanthanum nitrate for electron microscopy were used to demonstrate the changes in interstitial mucopolysaccharides (MPS). The lanthanum staining was done according to the method of Khan and Overton.Electron microscopy shows cytoplasmic lesions, vacules, swelling and degenerating mitochondria and intercellular spaces (IS) in the collecting tubule cells in DI and rats on milk diet.

Albumins as Embedding Media for Electron Microscopy

1969 ◽  
Vol 27 ◽  
pp. 422-423 ◽  
Author(s):  
J. L. Farrant ◽  
J. D. McLean
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
High Temperature ◽  
Biological Material ◽  
Globular Proteins ◽  
The Past ◽  
Antibody Staining ◽  
Thin Sectioning ◽  
Embedding Medium

For electron microscope techniques such as ferritin-labeled antibody staining it would be advantageous to have available a simple means of thin sectioning biological material without subjecting it to lipid solvents, impregnation with plastic monomers and their subsequent polymerization. With this aim in view we have re-examined the use of protein as an embedding medium. Gelatin which has been used in the past is not very satisfactory both because of its fibrous nature and the high temperature necessary to keep its solutions fluid. We have found that globular proteins such as the serum and egg albumins can be cross-linked so as to yield blocks which are suitable for ultrathin sectioning.

The intervertebral joint of the longnose gar, Lepisosteus osseus

1974 ◽  
Vol 52 (7) ◽  
pp. 803-804 ◽  
Author(s):  
H. U. Cameron
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Articular Cartilage ◽  
Hyaline Cartilage ◽  
Superficial Layer ◽  
Joint Cavity ◽  
Staining Techniques ◽  
Longnose Gar ◽  
Histological Staining ◽  
Scanning Electron

The intervertebral joint of the longnose gar has been examined by differential histological staining techniques and by scanning electron microscopy. The joint was found to be transitional, being neither a true diarthrosis nor a synchondrosis. The articular cartilage was found to consist of two layers, a superficial layer of fibrocartilage and a deeper layer of hyaline cartilage. The joint cavity was partially filled with fibrocartilaginous adhesions, the number of which varied from joint to joint.The degree of movement in each joint was minimal, in keeping with the heavily armored exoskeleton.

Electron microscopy of frozen-hydrated biological material

Nature ◽  
1986 ◽  
Vol 319 (6055) ◽  
pp. 631-636 ◽  
Author(s):  
Murray Stewart ◽  
Guy Vigers
Keyword(s):  
Electron Microscopy ◽  
Biological Material
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