The use of radio frequency sputter ion etching and scanning electron microscopy to study the internal structure of biological material

Vacuum ◽  
1969 ◽  
Vol 19 (11) ◽  
pp. 503-506 ◽  
Author(s):  
P.R. Stuart ◽  
J.S. Osborn ◽  
S.M. Lewis
Parasitology ◽  
1983 ◽  
Vol 86 (3) ◽  
pp. 477-480 ◽  
Author(s):  
D. L. Lee ◽  
C. D. Nicholls

SUMMARYPlasma etching has been used to strip away the cortical layers of the cuticle of adult Nippostrongylus brasiliensis to reveal the struts, with their supporting fibres, which are found in the fluid-filled middle layer of the cuticle, and the basal fibre layers. The etched specimens were studied by means of scanning electron microscopy. The results support earlier work, obtained by transmission electron microscopy, on the cuticle of this nematode. Plasma etching has been shown to have potential in studying the structure of nematodes.


2020 ◽  
Vol 15 (3) ◽  
Author(s):  
Muh Saleh

Lapisan tipis karbon amorf terhidrogenasi (a-C:H) telah berhasil ditumbuhkan di atas substrat kaca ITO dengan teknik Radio Frequency – Plasma Enhanced Chemical Vapor Deposition (RF–PECVD) pada frekuensi standar 13,56 MHz. Efek variasi parameter laju aliran gas metana (CH4) murni dari 40 sampai 100 sccm terhadap sifat optik dan sifat listrik lapisan a-C:H diselidiki dalam penelitian ini. Parameter temperatur substrat, tekanan deposisi, daya RF, dan waktu deposisi dibuat konstan pada nilai 100 0C, 450 mTorr, 15 W dan 120 menit. Jenis fase, ketebalan, celah pita energi dan konduktivitas listrik lapisan a-C:H masing-masing dikarakterisasi dengan Difraksi Sinar – X (XRD), Scanning Electron Microscopy (SEM), spektroskopi UV-Vis dan probe 4-titik (Four-Point Probe). Hasil karakterisasi dengan XRD menunjukkan bahwa struktur lapisan a-C:H yang diperoleh berfase amorf. Peningkatan laju aliran metana dari 40 sampai 100 sccm dengan selang 20 sccm telah meningkatkan ketebalan lapisan tipis a-C:H pada nilai 641,5 - 1256 nm dengan laju deposisi 5,43 - 10,47 nm/menit serta peningkatan konduktivitas listrik lapisan pada rentang nilai 6,90x10-2 - 7,43x10-2 S/cm yang berakibat pada penurunan celah pita energi lapisan dari 2,55 eV menjadi 2,00 eV dengan meningkatnya laju aliran metana.


1991 ◽  
Vol 5 (2) ◽  
pp. 443 ◽  
Author(s):  
RG Bland ◽  
DCF Rentz

The internal structure of the proventriculus of 17 species from 12 gryllacridid genera is described using light and scanning electron microscopy and is discussed in relation to its use as a taxonomic character. Structural differences were sufficient at 60 to 200 x to separate genera and species. Correlations between proventricular structure and food type are briefly discussed.


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