Correlated and combined confocal and conventional modes (and color coding) in tandem scanning reflected light microscopy

1988 ◽  
Vol 46 ◽  
pp. 992-993
Author(s):  
A. Boyde
Keyword(s):  
Light Microscope ◽  
Scanning Speed ◽  
Black Box ◽  
Color Coding ◽  
Confocal Scanning Laser Microscope ◽  
Area Of Interest ◽  
Reflected Light ◽  
Confocal Scanning ◽  
Confocal Scanning Laser ◽  
Conventional Light Microscope

It has been proposed that an overriding advantage of the “black-box” configuration of confocal scanning laser microscope (CSLM) is that it enables the use of all the familiar and conventional light microscope (LM) modes to discover a location of interest in a prepared, contrived, biological sample and then to switch to the confocal mode when desired. By implication, it has been assumed that this would not be possible in the Tandem Scanning Reflected Light Microscope (TSM). It is shown here that such suppositions are incorrect, and that correlations with different LM modes are very easily achieved in standard TSMs. In fact, because of the slow scanning speed of existing commercial CSLMs, it is not just an advantage to have other modes of image formation available, but a pure necessity, since finding the area of interest would otherwise be too difficult.

Measurements in 3D images

1991 ◽  
Vol 49 ◽  
pp. 408-409
Author(s):  
John C. Russ
Keyword(s):  
Three Dimensional ◽  
Image Plane ◽  
X Rays ◽  
Traditional Use ◽  
3D Images ◽  
Confocal Scanning Laser Microscope ◽  
Hard Materials ◽  
Depth Direction ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

Three-dimensional (3D) images consisting of arrays of voxels can now be routinely obtained from several different types of microscopes. These include both the transmission and emission modes of the confocal scanning laser microscope (but not its most common reflection mode), the secondary ion mass spectrometer, and computed tomography using electrons, X-rays or other signals. Compared to the traditional use of serial sectioning (which includes sequential polishing of hard materials), these newer techniques eliminate difficulties of alignment of slices, and maintain uniform resolution in the depth direction. However, the resolution in the z-direction may be different from that within each image plane, which makes the voxels non-cubic and creates some difficulties for subsequent analysis.

Fading of the diaminobenzidine reaction product in the confocal scanning laser microscope

1989 ◽  
Vol 47 ◽  
pp. 146-147
Author(s):  
JS Deitch ◽  
KL Smith ◽  
JW Swann ◽  
JN Turner
Keyword(s):  
Pyramidal Neurons ◽  
Light And Electron Microscopy ◽  
Scanning Laser ◽  
Laser Exposure ◽  
Confocal Scanning Laser Microscope ◽  
Fluorescent Markers ◽  
Before And After ◽  
Staining Protocol ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

Neurons labeled with horseradish peroxidase and reacted with diaminobenzidine (DAB) can be imaged using a confocal scanning laser microscope (CSLM) in the reflection mode. In contrast to fluorescent markers, the DAB reaction product is thought to be stable and can be observed by both light and electron microscopy. We have investigated the sensitivity of the DAB reaction product to laser irradiation, and present the spectrophotometric properties of DAB before and after exposure in the CSLM.Pyramidal neurons in slices of rat hippocampus were injected with biocytin (a biotin-lysine complex), fixed overnight in 4% paraformaldehyde, and vibratome sectioned at 75 μm. Biocytin was detected with avidin-HRP (1:200) in 0.5% Triton X-100, incubated in DAB (0.5 mg/ml) with or without 0.04% nickel ammonium sulfate (Ni), dehydrated, and imaged in a Bio Rad MRC-500 CSLM with an argon ion laser (488 and 514 nm). Spectrophotometric measurements of the soma were made on a Zeiss microspectrophotometer, as a function of laser exposure (100-1000 scans) and staining protocol.

scholarly journals Microbubble–liposome conjugate

2016 ◽  
Vol 3 ◽  
pp. 184954351667080 ◽  
Author(s):  
Ritu Malik ◽  
Ketan Pancholi ◽  
Andreas Melzer
Keyword(s):  
Drug Delivery ◽  
Drug Delivery System ◽  
Delivery System ◽  
Production Method ◽  
Drug Dose ◽  
Drug Delivery Vehicles ◽  
Confocal Scanning Laser Microscope ◽  
Mean Size ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

Liposome–microbubble conjugates are considered as better targeted drug delivery vehicles compared to microbubbles alone. The microbubble in the integrated drug delivery system delivers the drug intracellularly on the target, whereas the liposome component allows loading of high drug dose and extravasation through leaky vasculature. In this work, a new high yielding microbubble production method was used to prepare microbubbles for formulation of the liposome-conjugated drug delivery system. In formulation process, the prepared liposome of 200 nm diameter was attached to the microbubble surface using the avidin–biotin interaction. The analysis of the confocal scanning laser microscope images showed that approximately 8 × 108 microbubbles per millilitre (range: 2–7 μm, mean size 5 ± 0.5 μm) can be efficiently conjugated to the liposomes. The method of conjugation was found to be effective in attaching liposome to microbubbles.

Diagnostic Revolution of Microhematuria by Real Time Confocal Scanning Laser Microscope: Hyodo-Iino-Miyagawa Method, Third Report

Nephron ◽  
1995 ◽  
Vol 70 (2) ◽  
pp. 171-179 ◽  
Author(s):  
Toru Hyodo ◽  
Ikuo Miyagawa ◽  
Akihiro Iino ◽  
Koji Ono ◽  
Tsutomu Kuomi ◽  
...  
Keyword(s):  
Real Time ◽  
Scanning Laser ◽  
Confocal Scanning Laser Microscope ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

Twinning Behavior in AZ31-B Polycrystal Texture Subjected to In-Situ Bending Test

2010 ◽  
Vol 654-656 ◽  
pp. 715-718 ◽  
Author(s):  
Itsuya Sato ◽  
Seiji Miura ◽  
Tetsuo Mohri
Keyword(s):  
Bending Test ◽  
Confocal Scanning Laser Microscope ◽  
Compression Strain ◽  
Bending Process ◽  
Axis Compression ◽  
Confocal Scanning ◽  
Plane Plastic ◽  
Extension Strain ◽  
Confocal Scanning Laser

A commercial Mg alloy, AZ31B, has been used widely. In the texture of AZ31B sheet, each grain has its c-axis almost parallel to the sheet normal. Therefore, at the bending process of the sheet, basal slip system can not accommodate an in-plane plastic strain which is perpendicular to the c-axis of each grain. It is known that {10―,12} twin can be formed by applying an extension strain parallel to the c-axis, which is equivalent to the a-axis compression strain. So in the bending deformation of the AZ31B sheet with a texture microstructure, it is expected that {10―,12} twinning occurs. In this study, an in-situ bending test of AZ31B sheet with a texture was conducted under a confocal scanning laser microscope to observe twinning by applying compression stress along a direction almost perpendicular to the c-axis of grains. In addition, EBSD techniques were used for the analysis of crystal orientations. The process of twin development observed by the in-situ bending test can be summarized as follows; with the increase of the deformation strain, the total area of twins increases. However, it is noted that the growth of twins is apparent while the number of twins is almost constant during plastic bending deformmation. EBSD analysis suggested that twinning behavior obey Schmid’s law even in the polycrystal.

Fundamental Studies on the Transient Stages of Scale Growth in Fe-22 wt.% Cr Alloys

2009 ◽  
Vol 283-286 ◽  
pp. 425-431 ◽  
Author(s):  
Laura M. Fernandez Diaz ◽  
Jing Zhu ◽  
Gordon R. Holcomb ◽  
Paul D. Jablonski ◽  
David E. Alman ◽  
...  
Keyword(s):  
Short Circuit ◽  
Surface Oxidation ◽  
Parabolic Rate Constant ◽  
Rare Earth Oxide ◽  
Reactive Elements ◽  
Protective Oxide ◽  
Transient Time ◽  
Confocal Scanning Laser Microscope ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

It is known that additions of reactive elements such as Ce, La or Y improve the properties of protective oxide-scales on Ni and Fe based alloys [ - ] by increasing oxide adhesion, decreasing the transient time until a continuous Cr2O3 layer is formed and decreasing the parabolic rate constant. Nevertheless, the precise roles played by these reactive elements to improve scales and the precise mechanisms by which they are incorporated into the scale during the surface treatment processes are unknown. Although they are believed to be associated with transport properties in the scale, it is not clear how this occurs or why it improves oxidation resistance. This project is aimed to gain understanding of the scale evolution in Fe-22 wt.% Cr alloys at 800 oC in dry air during the transient stage after 15 minutes of oxidation. The effect of La (120 and 290 ppm) and Ce (270 and 610 ppm) additions added during melt-stage processing are investigated. The surface oxidation process was imaged in-situ through a Confocal Scanning Laser Microscope (CSLM) and the results were correlated with post-experiment characterization through FEG-SEM and FIB-SEM combined with 3D reconstruction. The roles of rare-earth oxide particles on nucleation of Cr2O3 and blockage of short-circuit diffusion paths in the oxide scale and underlying metal are discussed.

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