scholarly journals Rapid kinetics of endocytosis at rod photoreceptor synapses depends upon endocytic load and calcium

2014 ◽  
Vol 31 (3) ◽  
pp. 227-235 ◽  
Author(s):  
KARLENE M. CORK ◽  
WALLACE B. THORESON
Keyword(s):  
Tiger Salamander ◽  
Synaptic Ribbons ◽  
Rod Photoreceptor ◽  
Vesicle Release ◽  
Channel Activation ◽  
Pipette Solution ◽  
Rods And Cones ◽  
Rapid Kinetics ◽  
Kinetics Of ◽  
Endocytosis Mechanisms

AbstractRelease from rods is triggered by the opening of L-type Ca2+ channels that lie beneath synaptic ribbons. After exocytosis, vesicles are retrieved by compensatory endocytosis. Previous work showed that endocytosis is dynamin-dependent in rods but dynamin-independent in cones. We hypothesized that fast endocytosis in rods may also differ from cones in its dependence upon the amount of Ca2+ influx and/or endocytic load. We measured exocytosis and endocytosis from membrane capacitance (Cm) changes evoked by depolarizing steps in voltage clamped rods from tiger salamander retinal slices. Similar to cones, the time constant for endocytosis in rods was quite fast, averaging <200 ms. We manipulated Ca2+ influx and the amount of vesicle release by altering the duration and voltage of depolarizing steps. Unlike cones, endocytosis kinetics in rods slowed after increasing Ca2+ channel activation with longer step durations or more strongly depolarized voltage steps. Endocytosis kinetics also slowed as Ca2+ buffering was decreased by replacing BAPTA (10 or 1 mM) with the slower Ca2+ buffer EGTA (5 or 0.5 mM) in the pipette solution. These data provide further evidence that endocytosis mechanisms differ in rods and cones and suggest that endocytosis in rods is regulated by both endocytic load and local Ca2+ levels.

scholarly journals Rapid kinetics of myo-inositol trisphosphate binding and dissociation in cerebellar microsomes.

1994 ◽  
Vol 269 (47) ◽  
pp. 29642-29649
Author(s):  
Z Hannaert-Merah ◽  
J F Coquil ◽  
L Combettes ◽  
M Claret ◽  
J P Mauger ◽  
...  
Keyword(s):  
Inositol Trisphosphate ◽  
Rapid Kinetics ◽  
Kinetics Of

scholarly journals The Phosphoprotein Synapsin Ia Regulates the Kinetics of Dense-Core Vesicle Release

2021 ◽  
Vol 41 (13) ◽  
pp. 2828-2841
Author(s):  
Hui-Ju Yang ◽  
Pin-Chun Chen ◽  
Chien-Ting Huang ◽  
Tzu-Lin Cheng ◽  
Sheng-Ping Hsu ◽  
...  
Keyword(s):  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Release ◽  
Kinetics Of

scholarly journals An Early Intestinal Mucosal Source of Gamma Interferon Is Associated with Resistance to and Control of Cryptosporidium parvum Infection in Mice

2005 ◽  
Vol 73 (12) ◽  
pp. 8425-8428 ◽  
Author(s):  
Brett A. Leav ◽  
Masaru Yoshida ◽  
Kathleen Rogers ◽  
Seth Cohen ◽  
Nihal Godiwala ◽  
...  
Keyword(s):  
Cryptosporidium Parvum ◽  
Neutralizing Antibody ◽  
Cell Receptor ◽  
Intraepithelial Lymphocytes ◽  
Gamma Interferon ◽  
Susceptibility To Infection ◽  
Ifn Γ ◽  
Rapid Kinetics ◽  
And Control ◽  
Kinetics Of

ABSTRACT Resistance to and control of Cryptosporidium parvum infection in mice in the absence of adaptive immunity appears to be gamma interferon (IFN-γ) dependent. Using an IFN-γ-neutralizing antibody in a murine model, we demonstrated increased susceptibility to infection within 24 h. We correlated this early resistance and control with increased mucosal expression of IFN-γ and demonstrate that CD8+ T-cell receptor αβ intestinal intraepithelial lymphocytes express and secrete this cytokine shortly after infection. The rapid kinetics of IFN-γ expression and secretion by naive CD8+ T cells in response to a protozoan pathogen have not previously been demonstrated.

Rapid kinetics of polyethylene glycol-mediated fusion

1981 ◽  
Vol 7 (3) ◽  
pp. 281-287 ◽  
Author(s):  
P. S. Rabinovitch ◽  
T. H. Norwood
Keyword(s):  
Polyethylene Glycol ◽  
Rapid Kinetics ◽  
Kinetics Of

scholarly journals KINETICS OF ROD OUTER SEGMENT RENEWAL IN THE DEVELOPING MOUSE RETINA

1973 ◽  
Vol 58 (3) ◽  
pp. 650-661 ◽  
Author(s):  
Matthew M. LaVail
Keyword(s):  
Outer Segment ◽  
Photoreceptor Cells ◽  
Mouse Retina ◽  
Rod Photoreceptor ◽  
Adult Level ◽  
Rod Outer Segment ◽  
Equilibrium Length ◽  
Kinetics Of ◽  
Late Stages ◽  
Adult Rate

The kinetics of rod outer segment renewal in the developing retina have been investigated in C57BL/6J mice. Litters of mice were injected with [3H]amino acids at various ages and killed at progressively later time intervals. Plastic 1.5 µm sections of retina were studied by light microscope autoradiography. The rate of outer segment disk synthesis, as judged by labeled disk displacement away from the site of synthesis, is slightly greater than the adult level at 11–13 days of age; it rises to more than 1.6 times the adult rate between days 13 and 17, after which it falls to the adult level at 21–25 days. The rate of disk disposal, as measured by labeled disk movement toward the site of disposal, is less than 15% of the adult level at 11–13 days of age; it rises sharply to almost 70% of the adult level by days 13–15 and then more gradually approaches the adult rate. The net difference in rates of synthesis and disposal accounts for the rapid elongation of rod outer segments in the mouse between days 11 and 17 and the subsequent, more gradual elongation to the adult equilibrium length reached between days 19 and 25. The changing rate of outer segment disk synthesis characterizes the late stages of cytodifferentiation of the rod photoreceptor cells.

Rapid Kinetics of Cu(II) Adsorption/Desorption on Goethite

1994 ◽  
Vol 28 (8) ◽  
pp. 1422-1429 ◽  
Author(s):  
Paul R. Grossl ◽  
Donald L. Sparks ◽  
Calvin C. Ainsworth
Keyword(s):  
Rapid Kinetics ◽  
Adsorption Desorption ◽  
Kinetics Of
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