scholarly journals The Phosphoprotein Synapsin Ia Regulates the Kinetics of Dense-Core Vesicle Release

2021 ◽  
Vol 41 (13) ◽  
pp. 2828-2841
Author(s):  
Hui-Ju Yang ◽  
Pin-Chun Chen ◽  
Chien-Ting Huang ◽  
Tzu-Lin Cheng ◽  
Sheng-Ping Hsu ◽  
...  
Keyword(s):  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Release ◽  
Kinetics Of

scholarly journals Dense Core Vesicle Release: Controlling the Where as Well as the When

Genetics ◽  
2014 ◽  
Vol 196 (3) ◽  
pp. 601-604 ◽  
Author(s):  
Stephen Nurrish
Keyword(s):  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Release

scholarly journals UNC-108/RAB-2 and its effector RIC-19 are involved in dense core vesicle maturation in Caenorhabditis elegans

2009 ◽  
Vol 186 (6) ◽  
pp. 897-914 ◽  
Author(s):  
Marija Sumakovic ◽  
Jan Hegermann ◽  
Ling Luo ◽  
Steven J. Husson ◽  
Katrin Schwarze ◽  
...  
Keyword(s):  
Nervous System ◽  
Caenorhabditis Elegans ◽  
Vesicular Trafficking ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Release ◽  
C Elegans ◽  
Golgi Membranes ◽  
Human Diabetes ◽  
Synaptic Vesicle Release

Small guanosine triphosphatases of the Rab family regulate intracellular vesicular trafficking. Rab2 is highly expressed in the nervous system, yet its function in neurons is unknown. In Caenorhabditis elegans, unc-108/rab-2 mutants have been isolated based on their locomotory defects. We show that the locomotion defects of rab-2 mutants are not caused by defects in synaptic vesicle release but by defects in dense core vesicle (DCV) signaling. DCVs in rab-2 mutants are often enlarged and heterogeneous in size; however, their number and distribution are not affected. This implicates Rab2 in the biogenesis of DCVs at the Golgi complex. We demonstrate that Rab2 is required to prevent DCV cargo from inappropriately entering late endosomal compartments during DCV maturation. Finally, we show that RIC-19, the C. elegans orthologue of the human diabetes autoantigen ICA69, is also involved in DCV maturation and is recruited to Golgi membranes by activated RAB-2. Thus, we propose that RAB-2 and its effector RIC-19 are required for neuronal DCV maturation.

scholarly journals Munc13 controls the location and efficiency of dense-core vesicle release in neurons

2012 ◽  
Vol 199 (6) ◽  
pp. 883-891 ◽  
Author(s):  
Rhea van de Bospoort ◽  
Margherita Farina ◽  
Sabine K. Schmitz ◽  
Arthur de Jong ◽  
Heidi de Wit ◽  
...  
Keyword(s):  
Synaptic Vesicles ◽  
Hippocampal Neurons ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Wild Type ◽  
Vesicle Release ◽  
Prolonged Stimulation ◽  
And Function ◽  
Activity Dependent ◽  
Single Vesicle

Neuronal dense-core vesicles (DCVs) contain diverse cargo crucial for brain development and function, but the mechanisms that control their release are largely unknown. We quantified activity-dependent DCV release in hippocampal neurons at single vesicle resolution. DCVs fused preferentially at synaptic terminals. DCVs also fused at extrasynaptic sites but only after prolonged stimulation. In munc13-1/2–null mutant neurons, synaptic DCV release was reduced but not abolished, and synaptic preference was lost. The remaining fusion required prolonged stimulation, similar to extrasynaptic fusion in wild-type neurons. Conversely, Munc13-1 overexpression (M13OE) promoted extrasynaptic DCV release, also without prolonged stimulation. Thus, Munc13-1/2 facilitate DCV fusion but, unlike for synaptic vesicles, are not essential for DCV release, and M13OE is sufficient to produce efficient DCV release extrasynaptically.

scholarly journals Drosophila CAPS Is an Essential Gene that Regulates Dense-Core Vesicle Release and Synaptic Vesicle Fusion

Neuron ◽  
2001 ◽  
Vol 31 (3) ◽  
pp. 421-437 ◽  
Author(s):  
Robert Renden ◽  
Brent Berwin ◽  
Warren Davis ◽  
Kyoungsook Ann ◽  
Chin-Tang Chin ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Essential Gene ◽  
Vesicle Fusion ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Release ◽  
Synaptic Vesicle Fusion

scholarly journals Quantitative analysis of dense-core vesicle fusion in rodent CNS neurons

2021 ◽  
Vol 2 (1) ◽  
pp. 100325
Author(s):  
Alessandro Moro ◽  
Rein I. Hoogstraaten ◽  
Claudia M. Persoon ◽  
Matthijs Verhage ◽  
Ruud F. Toonen
Keyword(s):  
Quantitative Analysis ◽  
Vesicle Fusion ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Cns Neurons
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