scholarly journals The role of nitric oxide in priming-induced low-temperature tolerance in two genotypes of tomato

2013 ◽  
Vol 23 (2) ◽  
pp. 123-131 ◽  
Author(s):  
Rayhaneh Amooaghaie ◽  
Khorshid Nikzad
Keyword(s):  
Nitric Oxide ◽  
Seed Germination ◽  
Low Temperature ◽  
Seedling Growth ◽  
Sugar Content ◽  
Temperature Tolerance ◽  
Seedling Vigour ◽  
Positive Effects ◽  
Low Temperature Tolerance ◽  
Root And Shoot Length

AbstractThe present study was conducted to evaluate the contribution of nitric oxide (NO) to the priming effects on seed germination, seedling vigour and low-temperature tolerance of two tomato cultivars (Solanum lycopersicum, cv. Cherry and Falcato) under four different constant temperatures (10, 15, 20 and 25°C). Treatments included priming in a polyethylene glycol 6000 (PEG 6000) solution (osmopriming) or presoaking in sodium nitroprusside (SNP) solution, a donor of NO, for 24 h at 25°C. Suboptimal temperatures reduced seed germination and subsequent seedling growth, as well as amylase activity, in both cultivars, but Falcato was more sensitive to low temperature than Cherry. Both osmopriming and SNP treatments enhanced germination capacity, germination index, seedling root and shoot length, α- and β-amylase activities and sugar content under suboptimal temperatures, especially in Falcato. We observed similarities between the modes of action of the two priming treatments. Both treatments increased seed NO content, whereas their positive effects on germination and subsequent seedling growth under low-temperature stress was reversed by the addition of methylene blue (MB), a scavenger of NO. This is the first report that suggests that positive effects of priming are most likely mediated through NO signalling.

scholarly journals Putrescine Improve Low Temperature Tolerance of Fennel (Foeniculum Vulgare Mill.) Seeds

2015 ◽  
Vol 48 (1) ◽  
pp. 69-76 ◽  
Author(s):  
S.H. Mustafavi ◽  
F. Shekari ◽  
A. Abbasi
Keyword(s):  
Low Temperature ◽  
Dry Weight ◽  
Germination Percentage ◽  
Temperature Tolerance ◽  
Foeniculum Vulgare ◽  
Low Temperature Tolerance ◽  
Root And Shoot Length ◽  
Two Samples ◽  
Germination Behaviour ◽  
High Concentrations

Abstract The effects of polyamine priming on the germination behaviour of fennel at low temperatures were investigated. For preparing the putrescine pretreatments, seeds were divided into four parts. Two samples primed into putrescine (10 or 20 ppm) for 24 h, other samples were as controls. In order to eliminate the effect of water from test results, seeds were soaked in water only. After the priming, seeds were dried and used for germination test at 10 and 20 ºC. Except for seedling dry weight, all of the priming treatments improved germination performance and seedling growth of fennel seeds. Maximum germination percentage was achieved by 10 ppm Put application and lower value was observed in control seeds. About the energy of germination and mean germination time, polyamine treatments had better effect than hydropriming, but similar results was observed from seeds treated by 10 ppm Put and hydroprime on root and shoot length. Results showed that adequate presence of Put in the priming media had better than priming with water only. However, high concentrations of Put had not significant effect as well as 10 ppm Put. These results indicated that 10 ppm Put priming could be as an effective method to improve low temperature tolerance of fennel seeds.

Photosynthesis and antioxidant metabolism modulate the low-temperature resistance of seed germination in maize

2021 ◽  
Author(s):  
Aiju Meng ◽  
Daxing Wen ◽  
Chunqing Zhang
Keyword(s):  
Seed Germination ◽  
Low Temperature ◽  
Temperature Stress ◽  
Seedling Growth ◽  
Low Temperature Stress ◽  
Maize Seed ◽  
Down Regulation ◽  
Antioxidant Metabolism ◽  
Temperature Resistance ◽  
Low Temperature Resistance

Spring maize is usually subjected to low-temperature stress during seed germination, which retards seedling growth even if under a suitable temperature. However, the mechanism underlying maize seed germination under low-temperature stress modulating seedling growth after being transferred to normal temperature is still ambiguous. In this study, we used two maize inbred lines with different low-temperature resistance (SM and RM) to investigate the mechanism. The results showed that the SM line had higher lipid peroxidation and lower total antioxidant capacity and germination percentage than the RM line under low-temperature stress, which indicated that the SM line was more vulnerable to low-temperature stress. Further transcriptome analysis revealed that seed germination under low-temperature stress caused down-regulation of photosynthesis related gene ontology (GO) terms in two lines. Moreover, the SM line displayed down-regulation of ribosome and superoxide dismutase (SOD) related genes, whereas genes involved in SOD and vitamin B6 were up-regulated in the RM line. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that photosynthesis and antioxidant metabolism related pathways played important roles in seed germination in response to low-temperature stress, and the photosynthetic system displayed a higher damage degree in the SM line. Both qRT-PCR and physiological characteristics experiments showed similar results with transcriptome data. Taken together, we propose a model for maize seed germination in response to low-temperature stress.

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