Pre-meeting Sunday Short Courses: July 30

X-11: Scanning Cathodoluminescence Microscopy and Spectroscopy, Full Day: 9:00 AM–5:00 PM, Room 301/303.X-12: Digital Imaging 101: Scientific Imaging with Photoshop, Full day: 9:00 AM–5:00 PM, Room 302/304.X-13: Digital Imaging 102: Image Processing and Analysis, Full Day: 9:00 AM–5:00 PM, Room 306.X-14: Live Cell Imaging Using Fluorescence Methods, Full Day: 9:00 AM–5:00 PM, Room 305.X-15: What To Do with a Variable Pressure (VPSEM) or Environmental SEM (ESEM) And How To Do It (Or At Least How It Ought To Work), Full Day: 9:00 AM–5:00 PM, Room 307.X-16: 3-Dimensional Electron Microscopy (3DEM) in Life and Material Science—In-Depth Tutorial about Tomography—Basics and Methods, Full day: 9:00 AM–5:00 PM, Room 308.X-17 Failure Analysis and Evidence Preservation by Metallography, Half day: 9:00 AM–1:00 PM, Room 309/311.Special events: MSA and MAS presidential happenings, IMS Henry Clifton Sorby award and lecture, and art exhibit.

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PRE-MEETING SHORT COURSES: JULY 31

Microscopy and Microanalysis ◽

10.1017/s1431927605150302 ◽

2005 ◽

Vol 11 (I1) ◽

pp. 23-26

Keyword(s):

Freeze Substitution ◽

Specimen Preparation ◽

Digital Microscopy ◽

High Pressure Freezing ◽

Physical Sciences ◽

Special Events ◽

Nanoscale Imaging ◽

Art Exhibit ◽

Short Courses ◽

Full Day

Organizers: John Mansfield and Louis KerrAdditional fees required.SC01: Towards Nanoscale Imaging of Anything in VPSEM (including ESEM): From Basics to Current Practices. Full Day: 9:00 AM–5:00 PM, Room 317A.SC02: Image Processing and Analysis. Full Day: 9:00 AM–5:00 PM, Room 317B.SC03: Photoshop for Microscopy and Microanalysis. Full Day: 9:00 AM–5:00 PM, Room 318A.SC04: High Pressure Freezing Cryosectioning of Vitrified Samples for Tomography, and Freeze Substitution. Full Day: 9:00 AM–5:00 PM, Room 318B.SC05: Specimen Preparation for the Physical Sciences. Full Day: 9:00 AM–5:00 PM, Room 319A.SC06: Digital Microscopy and Image Analysis for Materials Characterization. Half Day: 9:00 AM–1:00 PM, Room 319B.SC07: Interpretation of Microstructure. Half-Day: 9:00 AM–1:00 PM, Room 323A.Special Events: Presidential happenings; IMS Henry Clifton Sorby award and lecture; and Art Exhibit.Educational Venues: Microscopic explorations: A workshop; and It's a Family Affair!

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Live Cell Imaging: 3-Dimensional Tracking of Non-blinking ‘Giant’ Quantum Dots in Live Cells (Adv. Funct. Mater. 30/2014)

Advanced Functional Materials ◽

10.1002/adfm.201470200 ◽

2014 ◽

Vol 24 (30) ◽

pp. 4795-4795 ◽

Cited By ~ 1

Author(s):

Aaron M. Keller ◽

Yagnaseni Ghosh ◽

Matthew S. DeVore ◽

Mary E. Phipps ◽

Michael H. Stewart ◽

...

Keyword(s):

Quantum Dots ◽

Live Cell Imaging ◽

Cell Imaging ◽

Live Cell ◽

Live Cells ◽

3 Dimensional

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3-Dimensional Organization and Dynamics of the Microsporidian Polar Tube Invasion Machinery

10.1101/2020.04.03.024240 ◽

2020 ◽

Author(s):

Pattana Jaroenlak ◽

Michael Cammer ◽

Alina Davydov ◽

Joseph Sall ◽

Mahrukh Usmani ◽

...

Keyword(s):

Live Cell Imaging ◽

High Speed ◽

Cell Imaging ◽

Host Cells ◽

Polar Tube ◽

3 Dimensional ◽

Germination Process ◽

Block Face ◽

Very High

Microsporidia, a divergent group of single-celled eukaryotic parasites, harness a specialized harpoon-like invasion apparatus called the polar tube (PT) to gain entry into host cells. The PT is tightly coiled within the transmissible extracellular spore, and is about 20 times the length of the spore. Once triggered, the PT is rapidly ejected and is thought to penetrate the host cell, acting as a conduit for the transfer of infectious cargo into the host. The organization of this specialized infection apparatus in the spore, how it is deployed, and how the nucleus and other large cargo are transported through the narrow PT are not well understood. Here we use serial block-face scanning electron microscopy to reveal the 3-dimensional architecture of the PT and its relative spatial orientation to other organelles within the spore. Using high-speed optical microscopy, we also capture and quantify the entire PT germination process in vitro. Our results show that the emerging PT experiences very high accelerating forces to reach velocities exceeding 300 μm.s-1, and that firing kinetics differ markedly between species. Live-cell imaging reveals that the nucleus, which is approximately 7 times larger than the diameter of the PT, undergoes extreme deformation to fit through the narrow tube, and moves at speeds comparable to PT extension. Our study sheds new light on the 3-dimensional organization, dynamics, and mechanism of PT extrusion, and shows how infectious cargo moves through the tube to initiate infection.

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