Assessment of genetic diversity of Guizotia abyssinica (L.f.) Cass. (Asteraceae) from Ethiopia using amplified fragment length polymorphism

2008 ◽  
Vol 6 (1) ◽  
pp. 41-51 ◽  
Author(s):  
Mulatu Geleta ◽  
Tomas Bryngelsson ◽  
Endashaw Bekele ◽  
Kifle Dagne
Keyword(s):  
Genetic Diversity ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Geographic Distance ◽  
Amplified Fragment Length Polymorphism ◽  
Principal Coordinate Analysis ◽  
Gene Bank ◽  
Guizotia Abyssinica ◽  
Level Analysis

Seventeen populations of niger (Guizotia abyssinica), representing all regions in Ethiopia where this crop is grown, were investigated using the amplified fragment length polymorphism (AFLP) technique, in order to determine the extent and distribution of its genetic diversity. A total of 539 AFLP loci were scored using seven primer combinations applied to 170 individual plants. Of these, 90% were polymorphic and all the individuals investigated were genetically unique. Despite the fact that most of the variation was within populations, populations were differentiated at a significant level (analysis of molecular variance; P < 0.001). There were no significant differences between populations in relation to the extent and altitude of cultivation. A significant positive correlation was revealed between Nei's standard genetic distance and geographic distance. Cluster analysis and principal coordinate analysis revealed that populations from the same regions were clustered together in most cases. Further collection of niger germplasm from areas underrepresented in gene bank collections is recommended.

scholarly journals Fluorescent amplified fragment length polymorphism (fAFLP) analyses and genetic diversity in Litopenaeus vannamei (Penaeidae)

2005 ◽  
Vol 28 (2) ◽  
pp. 267-270 ◽  
Author(s):  
Michelle Mantovani Gonçalves ◽  
Manoel Victor Franco Lemos ◽  
Pedro Manoel Galetti Junior ◽  
Patrícia Domingues de Freitas ◽  
Manuel Antonio Andrade Furtado Neto
Keyword(s):  
Genetic Diversity ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Litopenaeus Vannamei ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism

Genetic diversity of fringed brome (Bromus ciliatus) as determined by amplified fragment length polymorphism

2005 ◽  
Vol 83 (10) ◽  
pp. 1322-1328 ◽  
Author(s):  
Yong-Bi Fu ◽  
Bruce E. Coulman ◽  
Yasas S.N. Ferdinandez ◽  
Jacques Cayouette ◽  
Paul M. Peterson
Keyword(s):  
Genetic Diversity ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Habitat Restoration ◽  
Fragment Length Polymorphism ◽  
Natural Populations ◽  
Germplasm Conservation ◽  
Amplified Fragment Length Polymorphism ◽  
Large Area ◽  
Geographic Origins

Fringed brome ( Bromus ciliatus L.) is found in native stands throughout a large area of North America. Little is known about the genetic diversity of this species. The amplified fragment length polymorphism (AFLP) technique was applied to assess the genetic diversity of 16 fringed brome populations sampled in Canada from the provinces of Alberta, British Columbia, Quebec, and Saskatchewan. Four AFLP primer pairs were employed to screen 82 samples with four to six samples per population and 83 polymorphic AFLP bands scored for each sample. The frequencies of the scored bands in all assayed samples ranged from 0.01 to 0.99 and averaged 0.53. Analysis of molecular variance revealed that 52.6% of the total AFLP variation resided among the 16 populations and 20.6% among the four provinces. The five Quebec populations appeared to be genetically the most diverse and distinct. The AFLP variability observed was significantly associated with the geographic origins of the fringed brome populations. These findings are useful for sampling fringed brome germplasm from natural populations for germplasm conservation and should facilitate the development of genetically diverse regional cultivars for habitat restoration and revegetation.

Ommatissus lybicus (Hemiptera: Tropiduchidae), an economically important pest of date palm (Arecaceae) with highly divergent populations

2018 ◽  
Vol 150 (3) ◽  
pp. 378-392 ◽  
Author(s):  
Abdoolnabi Bagheri ◽  
Yaghoub Fathipour ◽  
Majeed Askari-Seyahooei ◽  
Mehrshad Zeinalabedini
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Date Palm ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
28S Rrna ◽  
Polymorphism Data ◽  
Coi Sequences

AbstractOmmatissus lybicus de Bergevin (Hemiptera: Tropiduchidae) is a key pest of date palm (Phoenix dactylifera Linnaeus; Arecaceae) with worldwide distribution and various management strategies. To study genetic diversity of date palm hopper, a series of experiments was conducted on genetic structure and genetic diversity of 15 geographic populations of O. lybicus (Abu Musa, Bam, Bushehr, Behbahan, Tezerj, Fin, Jiroft, Shahdad, Jahrom, Ghire Karzin, Ghasre Shirin, Iran; Pakistan; Oman; Egypt; and Tunisia) by amplified fragment length polymorphism, cytochrome c oxidase subunit I (COI), and 28S rRNA markers. Analysis of molecular variance analysis of amplified fragment length polymorphism data and COI sequences revealed a significant variation among O. lybicus populations (94.12% and 65.08% similarities for amplified fragment length polymorphism and COI, respectively). The 28S rDNA sequences from different populations were identical. Phylogenetic network inferred from amplified fragment length polymorphism data and COI sequences grouped two geographically close populations (Tezerj and Bam) in the two distinct clades while far apart geographical populations bunched in the same or close clades. These two populations experience repeated exposure to heavy pesticide applications annually. In conclusion, study of the genetic structure revealed a considerable variation between O. lybicus populations under intensive chemical strategies.

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