Chromatography with Dynamically Created Liquid “Stationary” Phases:  Methanol and Carbon Dioxide

2003 ◽  
Vol 75 (14) ◽  
pp. 3557-3562 ◽  
Author(s):  
Z. Luo ◽  
Y. Xiong ◽  
J. F. Parcher
2000 ◽  
Vol 51 (5-6) ◽  
pp. 283-293 ◽  
Author(s):  
S. Svensson ◽  
A. Karlsson ◽  
O. Gyllenhaal ◽  
J. Vessman

2009 ◽  
Vol 76 (4) ◽  
pp. 1168-1172 ◽  
Author(s):  
Ingrid Artin ◽  
David R. Mason ◽  
Carmen Pin ◽  
Jenny Schelin ◽  
Michael W. Peck ◽  
...  

ABSTRACT The antimicrobial gas carbon dioxide is frequently used in modified atmosphere packaging. In the present study, the effects of CO2 (10 to 70%, vol/vol) on gene expression (measured using quantitative reverse transcription-PCR and a whole-genome DNA microarray) and neurotoxin formation (measured using an enzyme-linked immunosorbent assay [ELISA]) by proteolytic Clostridium botulinum type A1 strain ATCC 3502 were studied during the growth cycle. Interestingly, in marked contrast to the situation with nonproteolytic C. botulinum types B and E, CO2 had little effect on any of these parameters. At all CO2 concentrations, relative expression of neurotoxin cluster genes peaked in the transition between exponential and stationary phases, with evidence of a second rise in expression in late stationary phase. Microarray analysis enabled identification of coding sequences whose expression profiles matched those of the neurotoxin cluster. Further research is needed to determine whether these are connected to neurotoxin formation or are merely growth phase associated.


2008 ◽  
Vol 1191 (1-2) ◽  
pp. 99-107 ◽  
Author(s):  
Norma M. Scully ◽  
Liam O. Healy ◽  
Tom O’Mahony ◽  
Jeremy D. Glennon ◽  
Benjamin Dietrich ◽  
...  

Author(s):  
K. C. Tsou ◽  
J. Morris ◽  
P. Shawaluk ◽  
B. Stuck ◽  
E. Beatrice

While much is known regarding the effect of lasers on the retina, little study has been done on the effect of lasers on cornea, because of the limitation of the size of the material. Using a combination of electron microscope and several newly developed cytochemical methods, the effect of laser can now be studied on eye for the purpose of correlating functional and morphological damage. The present paper illustrates such study with CO2 laser on Rhesus monkey.


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