Fast Screening of Low Molecular Weight Compounds by Thin-Layer Chromatography and “On-Spot” MALDI-TOF Mass Spectrometry

2004 ◽  
Vol 76 (7) ◽  
pp. 2144-2147 ◽  
Author(s):  
Leonardo S. Santos ◽  
Renato Haddad ◽  
Nelci F. Höehr ◽  
Ronaldo A. Pilli ◽  
Marcos N. Eberlin
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Low Molecular Weight ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Fast Screening ◽  
Low Molecular Weight Compounds ◽  
Layer Chromatography

THIN LAYER CHROMATOGRAPHY OF CARBOHYDRATE ACETATES

1962 ◽  
Vol 40 (6) ◽  
pp. 1043-1048 ◽  
Author(s):  
M. E. Tate ◽  
C. T. Bishop
Keyword(s):  
Molecular Weight ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Low Molecular Weight ◽  
Preparative Separation ◽  
Preparative Scale ◽  
Hydrophobic Compounds ◽  
Non Destructive ◽  
Layer Chromatography ◽  
Analytical Detection

Thin layer chromatography on silica gel G with benzene–methanol mixtures is a rapid method for the analytical or semimicro preparative separation of low molecular weight carbohydrate acetates. Some mixtures of inositol acetates are also resolved by the same procedure. A sensitive spray reagent has been developed for the analytical detection of acetates on the chromatographic plates and is based on the formation of the ferric hydroxamate of acetic acid. Oligosaccharide acetates are easily detected on a semimicro preparative scale by spraying the chromatographic plates with water, a general, non-destructive method for detection of hydrophobic compounds. The chromatograms can be run in 40 minutes and multiple development can be carried out to improve resolution when necessary.

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