scholarly journals Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry

10.3791/50757 ◽  
2013 ◽  
Author(s):  
Evan L. Pannkuk ◽  
Thomas S. Risch ◽  
Brett J. Savary
Keyword(s):  
Mass Spectrometry ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Preparative Thin Layer Chromatography ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Layer Chromatography

Analysis by HPLC – mass spectrometry of the indole compounds released by the ectomycorrhizal fungus Hebeloma hiemale in pure culture

1986 ◽  
Vol 64 (9) ◽  
pp. 1893-1897 ◽  
Author(s):  
R. Rouillon ◽  
G. Gay ◽  
J. Bernillon ◽  
J. Favre-Bonvin ◽  
G. Bruchet
Keyword(s):  
Mass Spectrometry ◽  
Acetic Acid ◽  
Carboxylic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Pure Culture ◽  
Preparative Thin Layer Chromatography ◽  
Ectomycorrhizal Fungus ◽  
Indole Compounds ◽  
Layer Chromatography

Indole compounds released by Hebeloma hiemale Bres. in pure culture were studied by cultivating the fungus on a nutrient solution supplemented or not with 1 mM tryptophan. Analytical thin-layer chromatography revealed numerous indole compounds in 7-week-old culture filtrates obtained in the presence of tryptophan. Such compounds were isolated by preparative thin-layer chromatography and purified by high performance liquid chromatography. The most abundant of them, i.e., indolyl-3-acetic acid, indolyl-3-carboxylic acid, and indole-3-aldehyde, were identified by mass spectrometry. Indole-3-aldehyde and indolyl-3-carboxylic acid, which were detected in filtrates throughout the culture period, might result from breakdown of indolyl-3-acetic acid by fungal exocellular enzymes.

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