Determination of Six Thyroid Hormones in the Brain and Thyroid Gland Using Isotope-Dilution Liquid Chromatography/Tandem Mass Spectrometry

2011 ◽  
Vol 83 (1) ◽  
pp. 417-424 ◽  
Author(s):  
Tatsuya Kunisue ◽  
Jeffrey W. Fisher ◽  
Kurunthachalam Kannan
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Thyroid Gland ◽  
Tandem Mass Spectrometry ◽  
Thyroid Hormones ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
The Brain

scholarly journals THE ROLE OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY − TANDEM MASS SPECTROMETRY IN OPTIMIZING DIAGNOSIS AND TREATMENT OF THYROID DISEASES

2018 ◽  
Vol 73 (4) ◽  
pp. 279-284
Author(s):  
Margarita S. Mikhina ◽  
Vitaliy A. Ioutsi ◽  
Ekaterina A. Troshina
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Thyroid Gland ◽  
Tandem Mass Spectrometry ◽  
Thyroid Hormones ◽  
High Performance ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Thyroid hormones play an integral role in growth, homeostasis, and maintenance of physiological functions and are necessary for normal development. Therefore, an accurate assessment of thyroid function is important for both diagnosis and treatment of thyroid disorders. Thyroid function is assessed by measuring thyroid-stimulating hormone (TSH) and thyroid hormones: thyroxine (T4), triiodothyronine (T3), total and free fractions. The concentration of thyroid hormones in patients without pathology of the thyroid gland remains constant and correlates with the level of hormones in tissues and their biological effects. However, most circulating hormones are bound to plasma proteins and only a small part is in free form, the most commonly used methods of immunoassay in this connection have limitations. The method of high performance liquid chromatography − tandem mass spectrometry (HPLC-MS/MS) successfully copes with this problem, which allows improving the specificity and accuracy of measurement of thyroid hormones. High performance liquid chromatography − tandem mass spectrometry allows to achieve specificity, accuracy for reliable determination of the level of thyroid hormones, increasing diagnostic capabilities. This method is especially important in the presence of thyroid gland pathology and factors affecting the binding of hormones to the protein.

A method for the analysis of six thyroid hormones in thyroid gland by liquid chromatography–tandem mass spectrometry

2010 ◽  
Vol 878 (21) ◽  
pp. 1725-1730 ◽  
Author(s):  
Tatsuya Kunisue ◽  
Jeffrey W. Fisher ◽  
Babatope Fatuyi ◽  
Kurunthachalam Kannan
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Thyroid Gland ◽  
Tandem Mass Spectrometry ◽  
Thyroid Hormones ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass
Sign in / Sign up

Export Citation Format

Share Document