Electrostatic Protein Immobilization Using Charged Polyacrylamide Gels and Cationic Detergent Microfluidic Western Blotting

2012 ◽  
Vol 84 (5) ◽  
pp. 2533-2540 ◽  
Author(s):  
Dohyun Kim ◽  
Kelly Karns ◽  
Samuel Q. Tia ◽  
Mei He ◽  
Amy E. Herr
Keyword(s):  
Western Blotting ◽  
Protein Immobilization ◽  
Polyacrylamide Gels ◽  
Cationic Detergent

Western blotting of histones from acid-urea-Triton-and sodium dodecyl sulfate-polyacrylamide gels

1987 ◽  
Vol 162 (2) ◽  
pp. 430-434 ◽  
Author(s):  
Jakob H. Waterborg ◽  
Rodney E. Harrington
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Western Blotting ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gels ◽  
Dodecyl Sulfate

An Investigation of Cross-Reactive Allergens and Antigens of Imperata cylindrica Using Western Blotting and ELISA Inhibition

2003 ◽  
Vol 15 (02) ◽  
pp. 062-067 ◽  
Author(s):  
Kaiser Bijli ◽  
B. Singh ◽  
Susheela Sridhara ◽  
S. Gaur ◽  
Naveen Arora
Keyword(s):  
Western Blotting ◽  
Imperata Cylindrica ◽  
Elisa Inhibition

Soluble Fibrin Complexes and Fibrinogen Heterogeneity in Diabetes mellitus

1980 ◽  
Vol 44 (03) ◽  
pp. 130-134 ◽  
Author(s):  
E B Tsianos ◽  
N E Stathakis
Keyword(s):  
Diabetes Mellitus ◽  
Molecular Weight ◽  
Plasma Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Gel Filtration ◽  
Lower Molecular Weight ◽  
Polyacrylamide Gels ◽  
Soluble Fibrin ◽  
Α2 Macroglobulin ◽  
Patients With Diabetes

SummaryThe presence of soluble fibrin complexes (SFC) measured by gel filtration of plasma on 4% agarose columns, fibrinogen heterogeneity on 3.5% SDS-polyacrylamide gels and the concentrations of several plasma proteins were evaluated in 39 patients with diabetes mellitus (DM) and 19 matched control subjects. A small but significant increase of SFC was found in DM (p<0.01). On individual basis 51.2% of the patients had increased SFC (>M + 2 SD of the controls). Polyacrylamide gel electrophoresis of the SFC showed no evidence of cross-linking or proteolysis. Plasma clots formed in the presence of EDTA and trasylol were analysed in SDS-polyacrylamide gels in a normal and two lower molecular weight fibrin bands (band I, II, III). The percentage of band I fibrinogen was in diabetics (65.3 ± 4.7%) lower than that of the controls (71.8 ± 4.5%) (p < 0.01). Fibrinogen levels, antithrombin III, α1-antitrypsin, α2-macroglobulin and plasminogen were significantly increased in DM. We suggest that in DM there is an enhancement of intravascular fibrin formation and accelerated fibrinogen degradation to lower molecular weight forms.

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