Detection of Mycobacterium tuberculosis Using a Capillary-Array Microsystem with Integrated DNA Extraction, Loop-Mediated Isothermal Amplification, and Fluorescence Detection

2013 ◽  
Vol 85 (9) ◽  
pp. 4698-4704 ◽  
Author(s):  
Dayu Liu ◽  
Guangtie Liang ◽  
Qiong Zhang ◽  
Bin Chen
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Dna Extraction ◽  
Fluorescence Detection ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Capillary Array

scholarly journals Development of an in-house loop-mediated isothermal amplification (LAMP) assay for detection of Mycobacterium tuberculosis and evaluation in sputum samples of Nepalese patients

2008 ◽  
Vol 57 (4) ◽  
pp. 439-443 ◽  
Author(s):  
Basu Dev Pandey ◽  
Ajay Poudel ◽  
Tomoko Yoda ◽  
Aki Tamaru ◽  
Naozumi Oda ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Lamp Assay ◽  
High Sensitivity ◽  
Isothermal Amplification ◽  
Nucleic Acid Amplification ◽  
Lamp Method ◽  
Rrna Gene ◽  
Predictive Values ◽  
Loop Mediated Isothermal Amplification ◽  
System A

A number of nucleic acid amplification assays (NAAs) have been employed to detect tubercle bacilli in clinical specimens for tuberculosis (TB) diagnosis. Among these, loop-mediated isothermal amplification (LAMP) is an NAA possessing superior isothermal reaction characteristics. In the present study, a set of six specific primers targeting the Mycobacterium tuberculosis 16S rRNA gene with high sensitivity was selected and a LAMP system (MTB-LAMP) was developed. Using this system, a total of 200 sputum samples from Nepalese patients were investigated. The sensitivity of MTB-LAMP in culture-positive samples was 100 % (96/96), and the specificity in culture-negative samples was 94.2 % (98/104, 95 % confidence interval 90.5–97.9 %). The positive and negative predictive values of MTB-LAMP were 94.1 and 100 %, respectively. These results indicate that this MTB-LAMP method may prove to be a powerful tool for the early diagnosis of TB.

Multiplex sample-to-answer detection of bacteria using a pipette-actuated capillary array comb with integrated DNA extraction, isothermal amplification, and smartphone detection

Lab on a Chip ◽  
2018 ◽  
Vol 18 (18) ◽  
pp. 2854-2864 ◽  
Author(s):  
Junhou Hui ◽  
Yin Gu ◽  
Yuanshou Zhu ◽  
Yanjing Chen ◽  
Shu-juan Guo ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Point Of Care ◽  
Isothermal Amplification ◽  
Point Of Care Testing ◽  
Capillary Array

A pipette-actuated capillary array comb system controlled and detected on a smartphone-based hand-held device was developed for point-of-care testing.

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