Ultrasensitive Detection of Telomerase Activity at the Single-Cell Level

2013 ◽  
Vol 85 (23) ◽  
pp. 11509-11517 ◽  
Author(s):  
Li-juan Wang ◽  
Yan Zhang ◽  
Chun-yang Zhang
Keyword(s):  
Single Cell ◽  
Telomerase Activity ◽  
Ultrasensitive Detection ◽  
Single Cell Level ◽  
Cell Level

DNA-fueled molecular machine for label-free and non-enzymatic ultrasensitive detection of telomerase activity

The Analyst ◽  
2016 ◽  
Vol 141 (16) ◽  
pp. 4855-4858 ◽  
Author(s):  
Panpan Sun ◽  
Xiang Ran ◽  
Chaoqun Liu ◽  
Chaoying Liu ◽  
Fang Pu ◽  
...  
Keyword(s):  
Single Cell ◽  
Cancer Cell ◽  
Telomerase Activity ◽  
Molecular Machine ◽  
Ultrasensitive Detection ◽  
Single Cell Level ◽  
Label Free ◽  
Enzymatic Method ◽  
Cell Level ◽  
Cell Extracts

A label-free and non-enzymatic method based on DNA-fueled molecular machine has been introduced for ultrasensitive detection of telomerase activity in cancer cell extracts even at the single-cell level.

Label-free and ultrasensitive detection of polynucleotide kinase activity at the single-cell level

2018 ◽  
Vol 54 (13) ◽  
pp. 1583-1586 ◽  
Author(s):  
Meng Liu ◽  
Fei Ma ◽  
Qianyi Zhang ◽  
Chun-yang Zhang
Keyword(s):  
Single Cell ◽  
Kinase Activity ◽  
Fluorescence Method ◽  
Kinase Assay ◽  
Ultrasensitive Detection ◽  
Single Cell Level ◽  
Label Free ◽  
Cell Level ◽  
Polynucleotide Kinase

We develop a label-free fluorescence method for the polynucleotide kinase assay at the single-cell level based on phosphorylation-triggered isothermal exponential amplification.

A modified exponential amplification reaction (EXPAR) with an improved signal-to-noise ratio for ultrasensitive detection of polynucleotide kinase

2019 ◽  
Vol 55 (53) ◽  
pp. 7611-7614 ◽  
Author(s):  
Yu-Peng Zhang ◽  
Yun-Xi Cui ◽  
Xiao-Yu Li ◽  
Yi-Chen Du ◽  
An-Na Tang ◽  
...  
Keyword(s):  
Single Cell ◽  
Kinase Activity ◽  
Signal To Noise Ratio ◽  
Ultrasensitive Detection ◽  
Single Cell Level ◽  
Signal To Noise ◽  
Cell Level ◽  
Polynucleotide Kinase ◽  
Noise Ratio ◽  
Amplification Reaction

We reported a modified exponential amplification reaction strategy and applied it to design an ultrasensitive biosensor for the detection of endogenous polynucleotide kinase activity at single-cell level.

Point-of-Care Assay of Telomerase Activity at Single-Cell Level via Gas Pressure Readout

2017 ◽  
Vol 89 (16) ◽  
pp. 8311-8318 ◽  
Author(s):  
Yanjun Wang ◽  
Luzhu Yang ◽  
Baoxin Li ◽  
Chaoyong James Yang ◽  
Yan Jin
Keyword(s):  
Single Cell ◽  
Point Of Care ◽  
Telomerase Activity ◽  
Gas Pressure ◽  
Single Cell Level ◽  
Cell Level ◽  
Point Of Care Assay

SERS assay of telomerase activity at single-cell level and colon cancer tissues via quadratic signal amplification

2016 ◽  
Vol 77 ◽  
pp. 673-680 ◽  
Author(s):  
Muling Shi ◽  
Jing Zheng ◽  
Changhui Liu ◽  
Guixiang Tan ◽  
Zhihe Qing ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Single Cell ◽  
Signal Amplification ◽  
Telomerase Activity ◽  
Single Cell Level ◽  
Cell Level ◽  
Quadratic Signal ◽  
Cancer Tissues

FRET investigation toward DNA tetrahedron-based ratiometric analysis of intracellular telomerase activity

2019 ◽  
Vol 7 (11) ◽  
pp. 1926-1932 ◽  
Author(s):  
Fanyu Meng ◽  
Hua Chai ◽  
Xiaoyi Ma ◽  
Yuguo Tang ◽  
Peng Miao
Keyword(s):  
Single Cell ◽  
Telomerase Activity ◽  
Single Cell Level ◽  
Cell Level ◽  
Ratiometric Sensing ◽  
Dna Tetrahedron

Ratiometric sensing of telomerase activity is realized at a single-cell level based on a novel DNA nanoprobe reconciling an extension primer, a DNA tetrahedron and a flare probe.

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