Plasmon-Induced Enhancement in Analytical Performance Based on Gold Nanoparticles Deposited on TiO2Film

The sensitivity and reproducibility of the lateral flow assay can be influenced by multiple factors, such as the size of gold nanoparticles (GNPs) employed. Here, we evaluated the analytical performance of single-sized and mixed-sized GNPs using a simple lateral flow assay (LFA) platform. This platform was used as a model assay to diagnose albumin levels and demonstrate the analytical performance of single-sized and mixed-sized GNPs in LFA tests. Two sizes of GNPs@anti-bovine serum albumin (BSA) conjugate proteins were mixed at different ratios. The unique optical properties of the GNPs induced a distinguishing color-shedding effect on the single- and mixed-sized GNPs@anti-BSA conjugates interacting with the target analyte BSA spotted on the test line. The use of mixed-sized GNPs@anti-BSA conjugates enhanced signal relative to the 20 nm GNPs, and provided superior stability compared with solely employing the large GNPs (50 nm). The proposed platform in this study could provide an efficient BSA detection mechanism that can be utilized as a model biomarker for confronting chronic kidney disease.

Download Full-text

Electrochemical sensors of octylphenol based on molecularly imprinted poly(3,4-ethylenedioxythiophene) and poly(3,4-ethylenedioxythiophene–gold nanoparticles)

RSC Advances ◽

10.1039/c5ra08094k ◽

2015 ◽

Vol 5 (71) ◽

pp. 57671-57677 ◽

Cited By ~ 8

Author(s):

Yanhui Pan ◽

Faqiong Zhao ◽

Baizhao Zeng

Keyword(s):

Gold Nanoparticles ◽

Electrochemical Sensors ◽

Graphene Nanoribbons ◽

Analytical Performance ◽

Molecularly Imprinted ◽

Octyl Phenol

Two molecularly imprinted electrochemical sensors are fabricated by using EDOT and EDOT–AuNPs as monomers, respectively. The sensors show good analytical performance for OP sensing. Note: graphene nanoribbons (GNRs), 3,4-ethylenedioxythiophene (EDOT), 4-tert-octyl-phenol (OP).

Download Full-text

Performance comparison of single and mixed-sized gold nanoparticles on straightforward lateral flow assay for albumin detection

10.3762/bxiv.2020.144.v1 ◽

2020 ◽

Author(s):

Sasima Chotithammakul ◽

Dakrong Pissuwan

Keyword(s):

Gold Nanoparticles ◽

Signal Amplification ◽

Test Line ◽

Testing Procedure ◽

Performance Comparison ◽

Lateral Flow ◽

Lateral Flow Assay ◽

Analytical Performance ◽

Detection Mechanism ◽

Multiple Factors

The sensitivity of the lateral flow assay can be influenced by multiple factors, such as the size of gold nanoparticles (GNPs) employed, which affect the sensitivity and reproducibility of the assay testing procedure. Here, we evaluated the analytical performance of single-sized and mixed-sized GNPs using a simple lateral flow assay (LFA) platform. This platform was used as a model assay to diagnose albumin levels and demonstrate the analytical performance of single-sized and mixed-sized GNPs in LFA tests. Two sizes of GNPs@anti-bovine serum albumin (BSA) conjugate proteins were mixed at different ratios. The unique optical properties of the GNPs induced a distinguishing color-shedding effect on the single and mixed-sized GNPs@anti-BSA conjugates interacting with the target analyte BSA spotted on the test line. The use of mixed-sized GNPs@anti-BSA conjugates enhanced signal amplification, and provided superior stability compared with solely employing the large GNPs (50 nm). The proposed platform in this study could provide an efficient BSA detection mechanism that can be utilized as a biomarker for confronting chronic kidney disease.

Download Full-text