Development of a Standardized Microflow LC Gradient to Enable Sensitive and Long-Term Detection of Synthetic Anabolic-Androgenic Steroids for High-Throughput Doping Controls

2021 ◽  
Author(s):  
Vivian Delcourt ◽  
Patrice Garcia ◽  
Isabelle Pottier ◽  
Nasrine Mansoibou ◽  
Nicolai Bache ◽  
...  
Keyword(s):  
High Throughput ◽  
Anabolic Androgenic Steroids ◽  
Androgenic Steroids

P2545Long-term use of anabolic-androgenic steroids in male weightlifters is associated with left ventricular systolic dysfunction

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
R Abdullah ◽  
A Bjornebekk ◽  
L Dejgaard ◽  
L Hauger ◽  
T Edvardsen ◽  
...  
Keyword(s):  
Left Ventricular Mass ◽  
Systolic Function ◽  
Left Ventricular Systolic Function ◽  
Left Ventricular ◽  
Left Ventricular Ejection ◽  
Anabolic Androgenic Steroids ◽  
Ventricular Systolic Function ◽  
Ventricular Mass ◽  
Androgenic Steroids

Abstract Background Illicit use of anabolic-androgenic steroids (AAS) is widespread and has adverse psychological and cardiovascular effects. Few well-powered studies have explored the effect of long-term AAS-use on left ventricular systolic function. Purpose To explore the relationship between long-term use of AAS and left ventricular mass and systolic function. Methods We included male weightlifters with a history of >1 year of cumulative AAS-use and male weightlifters unexposed to AAS. We performed echocardiography in all and assessed left ventricular mass using 2D echocardiographic linear measurements and Cube formula, left ventricular ejection fraction (EF) ad modum Simpson, and left ventricular global longitudinal strain (GLS) by speckle-tracking echocardiography. Results We recruited 100 male weightlifters, 58 of whom were previous or current AAS-users with mean±SD AAS-use of 10.4±7.0 years, and 42 unexposed weightlifters. There were no difference in age (35.5±9.2 vs. 35.3±7.5 years, p=0.8) nor body mass index (BMI) (BMI 31.4±5.0 vs. 30.1±3.5, p=0.6) between AAS-exposed and unexposed weightlifters. Compared with unexposed weightlifters, AAS-exposed weightlifters demonstrated thicker interventricular septum (11.2±2.4 vs. 9.2±1.3 mm, p<0.001), thicker left ventricular posterior wall dimension (10.1±2.1 vs. 8.9±1.3 mm, p<0.001), and higher left ventricular mass index (99.7±25.4 vs. 78.4±12.1 g/m2, p<0.001). Both left ventricular EF and left ventricular GLS were decreased in AAS-exposed weightlifters compared with unexposed weightlifters (49±9 vs. 53±6%, p=0.02, and −15.6±2.6 vs. −18.3±2.1%, p<0.001) (Figure). Conclusion AAS use in male weightlifters was associated with increased left ventricular mass and impaired left ventricular systolic function. Our results suggest considerable adverse cardiac effects of AAS use, but the results need confirmation in prospective observational trials.

scholarly journals Anabolic-androgenic steroids cycle administration decreases anxious-like behavior but does not affect long-term memory acquisition in rats

2019 ◽  
Vol 41 (1) ◽  
pp. 45014
Author(s):  
Luís Flávio Souza de Oliveira ◽  
Miriam Staudt ◽  
Michel Mansur Machado ◽  
Alexandre Meneghello Fuentefria
Keyword(s):  
Anabolic Androgenic Steroids ◽  
Long Term Memory ◽  
Term Memory ◽  
Memory Acquisition ◽  
Androgenic Steroids

scholarly journals Long-Term Anabolic Androgenic Steroid Use Is Associated with Increased Atrial Electromechanical Delay in Male Bodybuilders

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Mustafa Akçakoyun ◽  
Elnur Alizade ◽  
Recep Gündoğdu ◽  
Mustafa Bulut ◽  
Mehmet Mustafa Tabakcı ◽  
...  
Keyword(s):  
Anabolic Androgenic Steroids ◽  
Anabolic Androgenic Steroid ◽  
Electromechanical Delay ◽  
Steroid Use ◽  
Atrial Electromechanical Delay ◽  
Androgenic Steroid ◽  
Androgenic Steroids

We investigated the effect of long-term supraphysiologic doses of anabolic androgenic steroids (AAS) on atrial electromechanical delay (AEMD) in male bodybuilders. We clearly demonstrated that long-term consumption of supraphysiologic doses of AAS is associated with higher values of inter- and intra-AEMD in healthy young bodybuilders.

scholarly journals New Insights into the Metabolism of Methyltestosterone and Metandienone: Detection of Novel A-ring Reduced Metabolites

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1354
Author(s):  
Steffen Loke ◽  
Lingyu Liu ◽  
Maxi Wenzel ◽  
Heike Scheffler ◽  
Michele Iannone ◽  
...  
Keyword(s):  
Anabolic Androgenic Steroids ◽  
Doping Analysis ◽  
Screening Procedures ◽  
Androgenic Steroids ◽  
Orally Active ◽  
Targeted Approach

Metandienone and methyltestosterone are orally active anabolic-androgenic steroids with a 17α-methyl structure that are prohibited in sports but are frequently detected in anti-doping analysis. Following the previously reported detection of long-term metabolites with a 17ξ-hydroxymethyl-17ξ-methyl-18-nor-5ξ-androst-13-en-3ξ-ol structure in the chlorinated metandienone analog dehydrochloromethyltestosterone (“oral turinabol”), in this study we investigated the formation of similar metabolites of metandienone and 17α-methyltestosterone with a rearranged D-ring and a fully reduced A-ring. Using a semi-targeted approach including the synthesis of reference compounds, two diastereomeric substances, viz. 17α-hydroxymethyl-17β-methyl-18-nor-5β-androst-13-en-3α-ol and its 5α-analog, were identified following an administration of methyltestosterone. In post-administration urines of metandienone, only the 5β-metabolite was detected. Additionally, 3α,5β-tetrahydro-epi-methyltestosterone was identified in the urines of both administrations besides the classical metabolites included in the screening procedures. Besides their applicability for anti-doping analysis, the results provide new insights into the metabolism of 17α-methyl steroids with respect to the order of reductions in the A-ring, the participation of different enzymes, and alterations to the D-ring.

scholarly journals Doping Practices in International Weightlifting: Analysis of sanctioned athletes/support personnel from 2008-2019 and retesting of samples from the 2008 and 2012 Olympic Games

2020 ◽  
Author(s):  
Alexander Kolliari-Turner ◽  
Brian Oliver ◽  
Giscard Lima ◽  
John P. Mills ◽  
Guan Wang ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Regional Differences ◽  
Olympic Games ◽  
Anabolic Androgenic Steroids ◽  
Sample Storage ◽  
Support Personnel ◽  
In Doping ◽  
Targeted Testing ◽  
Androgenic Steroids

Abstract Background The pervasiveness of doping and findings of anti-doping corruption threaten weightlifting’s position at the 2024 Olympics. Analysing the practices of doping weightlifters could identify patterns in doping that assist in future detection.Methods We analysed publicly available data on sanctioned athletes/support personnel from the International Weightlifting Federation (IWF) between 2008–2019 and retrospective Anti-Doping Rule Violations (ADRVs) from the 2008 and 2012 Olympics.Results There were 565 sanctions between 2008–2019 of which 82% related to exogenous and endogenous Anabolic Androgenic Steroids (AAS). The distribution of exogenous AAS, endogenous AAS and other detected substances varied by IWF Continental Federation (p ≤ 0.05) with Europe (74%, 11%, 15%) and Asia (70%, 15%, 15%) showing a higher detection of exogenous AAS compared to Pan America (37%, 30%, 33%) and Africa (50%, 17%, 33%). When looking at the 10 most detected substances, the nations with the highest number of sanctions (range 17–35) all had at least one overrepresented substance that accounted for 38–60% of all detected substances. The targeted re-analysis of samples from the 2008 and 2012 Olympics due to the discovery of long-term metabolites for exogenous AAS has to date produced 61 retrospective ADRVs in weightlifting. Of these, 34 were original medallists (9 Gold, 10 Silver and 15 Bronze), the highest of any sport. The exogenous AAS Dehydrochloromethyltestosterone and Stanozolol accounted for 83% of detected substances and were present in 95% of these samples.Conclusion Based on these findings of regional differences in doping practices, weightlifting would benefit from the targeted testing of certain regions and continuing investment in long-term sample storage as the sensitivity and specificity of detection continues to improve.

scholarly journals Doping Practices in International Weightlifting: Analysis of sanctioned athletes/support personnel from 2008-2019 and retesting of samples from the 2008 and 2012 Olympic Games

2020 ◽  
Author(s):  
Alexander Kolliari-Turner ◽  
Brian Oliver ◽  
Giscard Lima ◽  
John P. Mills ◽  
Guan Wang ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Regional Differences ◽  
Olympic Games ◽  
Anabolic Androgenic Steroids ◽  
Sample Storage ◽  
Support Personnel ◽  
In Doping ◽  
Targeted Testing ◽  
Androgenic Steroids

Abstract Background: The pervasiveness of doping and findings of anti-doping corruption threaten weightlifting’s position at the 2024 Olympic Games. Analysing the practices of doping in weightlifters could identify patterns in doping that assist in future detection. Methods: We analysed publicly available data on sanctioned athletes/support personnel from the International Weightlifting Federation between 2008-2019 and announced retrospective Anti-Doping Rule Violations (ADRVs) from the 2008 and 2012 Olympic Games. Results: There were 565 sanctions between 2008-2019 of which 82% related to the detection of exogenous Anabolic Androgenic Steroids (AAS) metabolites and markers indicating endogenous AAS usage. The detection of exogenous AAS metabolites, markers of endogenous AAS usage and other substance metabolites varied by IWF Continental Federation (p ≤ 0.05) with Europe (74%, 11%, 15%) and Asia (70%, 15%, 15%) showing a higher detection of exogenous AAS compared to Pan America (37%, 30%, 33%) and Africa (50%, 17%, 33%). When looking at the 10 most detected substances, the nations with the highest number of sanctions (range 17-35) all had at least one overrepresented substance that accounted for 38-60% of all detected substances. The targeted re-analysis of samples from the 2008 and 2012 Olympic Games due to the discovery of long-term metabolites for exogenous AAS resulted in 61 weightlifters producing retrospective ADRVs. This includes 34 original medallists (9 Gold, 10 Silver and 15 Bronze), the highest of any sport identified by Olympic Games sample re-testing. The exogenous AAS Dehydrochloromethyltestosterone and Stanozolol accounted for 83% of detected substances and were present in 95% of these samples. Conclusion: Based on these findings of regional differences in doping practices, weightlifting would benefit from the targeted testing of certain regions and continuing investment in long-term sample storage as the sensitivity and specificity of detection continues to improve.

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