scholarly journals 7-Aminocoumarin-4-acetic Acid as a Fluorescent Probe for Detecting Bacterial Dipeptidyl Peptidase Activities in Water-in-Oil Droplets and in Bulk

2021 ◽  
Author(s):  
Akihiro Nakamura ◽  
Nobuyuki Honma ◽  
Yuma Tanaka ◽  
Yoshiyuki Suzuki ◽  
Yosuke Shida ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Fluorescent Probe ◽  
Dipeptidyl Peptidase ◽  
Oil Droplets

scholarly journals Correlation of apoptosis with change in intracellular labile Zn(II) using zinquin [(2-methyl-8-p-toluenesulphonamido-6-quinolyloxy)acetic acid], a new specific fluorescent probe for Zn(II)

1993 ◽  
Vol 296 (2) ◽  
pp. 403-408 ◽  
Author(s):  
P D Zalewski ◽  
I J Forbes ◽  
W H Betts
Keyword(s):  
Image Analysis ◽  
Acetic Acid ◽  
Chronic Lymphocytic Leukaemia ◽  
Fluorescent Probe ◽  
Dna Fragmentation ◽  
Lymphocytic Leukaemia ◽  
Morphological Changes ◽  
Threshold Concentration ◽  
Video Image ◽  
Substantial Heterogeneity

Zinquin [(2-methyl-8-p-toluenesulphonamido-6-quinolyloxy)-acetic acid], a membrane-permeant fluorophore specific for Zn(II), was used with spectrofluorimetry and video image analysis to reveal and quantify labile intracellular Zn. Zinquin labelled human chronic-lymphocytic-leukaemia lymphocytes, rat splenocytes and thymocytes with a weak diffuse fluorescence that was quenched when intracellular Zn was chelated with NNN‘N’-tetrakis-(2-pyridylmethyl)ethylenediamine (TPEN) and was greatly intensified by pretreatment of cells with the Zn ionophore pyrithione and exogenous Zn. There was substantial heterogeneity of labile Zn among ionophore-treated cells, and fluorescence was largely extranuclear. The average contents of labile Zn in human leukaemic lymphocytes, rat splenocytes and rat thymocytes were approx. 20, 31 and 14 pmol/10(6) cells respectively. Morphological changes and internucleosomal DNA fragmentation indicated substantial apoptosis in these cells when the level of intracellular labile Zn was decreased by treatment with TPEN. Conversely, increasing labile Zn by pretreatment with Zn plus pyrithione suppressed both spontaneous DNA fragmentation and that induced by the potent apoptosis-induced agents colchicine and dexamethasone. These results suggest that prevention of apoptosis is a function of labile Zn, and that a reduction below a threshold concentration in this Zn pool induces apoptosis.

scholarly journals Detection of Early Adenocarcinoma of the Esophagogastric Junction by Spraying an Enzyme-Activatable Fluorescent Probe Targeting Dipeptidyl Peptidase-IV

2019 ◽  
Author(s):  
Keiko Yamamoto ◽  
Shunsuke Ohnishi ◽  
Takeshi Mizushima ◽  
Junichi Kodaira ◽  
Masayoshi Ono ◽  
...  
Keyword(s):  
Intestinal Metaplasia ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Esophagogastric Junction ◽  
Dipeptidyl Peptidase ◽  
Clinical Samples ◽  
Specific Sequence ◽  
Dpp Iv ◽  
Early Adenocarcinoma ◽  
Sensitivity Specificity

Abstract Background: It is still difficult to detect and diagnose early adenocarcinoma of the esophagogastric junction (EGJ) using conventional endoscopy or image-enhanced endoscopy. A glutamylprolyl hydroxymethyl rhodamine green (EP-HMRG) fluorescent probe that can be enzymatically activated to become fluorescent after the cleavage of a dipeptidyl peptidase (DPP)-IV-specific sequence has been developed and is reported to be useful for the detection of squamous cell carcinoma of the head and neck, and esophagus; however, there is a lack of studies that focuses on detecting EGJ adenocarcinoma by fluorescence molecular imaging. Therefore, we investigated the visualization of early EGJ adenocarcinoma by applying EP-HMRG and using clinical samples resected by endoscopic submucosal dissection (ESD). Methods: Fluorescence imaging with EP-HMRG was performed in 21 clinical samples resected by ESD, and the fluorescence intensity of the tumor and non-tumor regions of interest was prospectively measured. Immunohistochemistry was also performed to determine the expression of DPP-IV. Results: Fluorescence imaging of the clinical samples showed that the tumor lesions were visualized within a few minutes after the application of EP-HMRG, with a sensitivity, specificity, and accuracy of 85.7%, 85.7%, and 85.7%, respectively. However, tumors with a background of intestinal metaplasia did not have a sufficient contrast-to-background ratio since complete intestinal metaplasia also expresses DPP-IV. Immunohistochemistry measurements revealed that all fluorescent tumor lesions expressed DPP-IV. Conclusions: Fluorescence imaging with EP-HMRG could be useful for the detection of early EGJ adenocarcinoma lesions that do not have a background of intestinal metaplasia.

scholarly journals Detection of Early Adenocarcinoma of the Esophagogastric Junction by Spraying an Enzyme-Activatable Fluorescent Probe Targeting Dipeptidyl Peptidase-IV

2020 ◽  
Author(s):  
Keiko Yamamoto ◽  
Shunsuke Ohnishi ◽  
Takeshi Mizushima ◽  
Junichi Kodaira ◽  
Masayoshi Ono ◽  
...  
Keyword(s):  
Intestinal Metaplasia ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Esophagogastric Junction ◽  
Dipeptidyl Peptidase ◽  
Clinical Samples ◽  
Specific Sequence ◽  
Dpp Iv ◽  
Early Adenocarcinoma ◽  
Sensitivity Specificity

Abstract Background: It is still difficult to detect and diagnose early adenocarcinoma of the esophagogastric junction (EGJ) using conventional endoscopy or image-enhanced endoscopy. A glutamylprolyl hydroxymethyl rhodamine green (EP-HMRG) fluorescent probe that can be enzymatically activated to become fluorescent after the cleavage of a dipeptidyl peptidase (DPP)-IV-specific sequence has been developed and is reported to be useful for the detection of squamous cell carcinoma of the head and neck, and esophagus; however, there is a lack of studies that focuses on detecting EGJ adenocarcinoma by fluorescence molecular imaging. Therefore, we investigated the visualization of early EGJ adenocarcinoma by applying EP-HMRG and using clinical samples resected by endoscopic submucosal dissection (ESD). Methods: Fluorescence imaging with EP-HMRG was performed in 21 clinical samples resected by ESD, and the fluorescence intensity of the tumor and non-tumor regions of interest was prospectively measured. Immunohistochemistry was also performed to determine the expression of DPP-IV. Results: Fluorescence imaging of the clinical samples showed that the tumor lesions were visualized within a few minutes after the application of EP-HMRG, with a sensitivity, specificity, and accuracy of 85.7%, 85.7%, and 85.7%, respectively. However, tumors with a background of intestinal metaplasia did not have a sufficient contrast-to-background ratio since complete intestinal metaplasia also expresses DPP-IV. Immunohistochemistry measurements revealed that all fluorescent tumor lesions expressed DPP-IV. Conclusions: Fluorescence imaging with EP-HMRG could be useful for the detection of early EGJ adenocarcinoma lesions that do not have a background of intestinal metaplasia.

scholarly journals Detection of Early Adenocarcinoma of the Esophagogastric Junction by Spraying an Enzyme-Activatable Fluorescent Probe Targeting Dipeptidyl Peptidase-IV

2019 ◽  
Author(s):  
Keiko Yamamoto ◽  
Shunsuke Ohnishi ◽  
Takeshi Mizushima ◽  
Junichi Kodaira ◽  
Masayoshi Ono ◽  
...  
Keyword(s):  
Intestinal Metaplasia ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Esophagogastric Junction ◽  
Dipeptidyl Peptidase ◽  
Clinical Samples ◽  
Specific Sequence ◽  
Dpp Iv ◽  
Early Adenocarcinoma ◽  
Sensitivity Specificity

Abstract Background: It is still difficult to detect and diagnose early adenocarcinoma of the esophagogastric junction (EGJ) using conventional endoscopy or image-enhanced endoscopy. A glutamylprolyl hydroxymethyl rhodamine green (EP-HMRG) fluorescent probe that can be enzymatically activated to become fluorescent after the cleavage of a dipeptidyl peptidase (DPP)-IV-specific sequence has been developed and is reported to be useful for the detection of squamous cell carcinoma of the head and neck, and esophagus; however, there is a lack of studies that focuses on detecting EGJ adenocarcinoma by fluorescence molecular imaging. Therefore, we investigated the visualization of early EGJ adenocarcinoma by applying EP-HMRG and using clinical samples resected by endoscopic submucosal dissection (ESD). Methods: Fluorescence imaging with EP-HMRG was performed in 21 clinical samples resected by ESD, and the fluorescence intensity of the tumor and non-tumor regions of interest was prospectively measured. Immunohistochemistry was also performed to determine the expression of DPP-IV. Results: Fluorescence imaging of the clinical samples showed that the tumor lesions were visualized within a few minutes after the application of EP-HMRG, with a sensitivity, specificity, and accuracy of 85.7%, 85.7%, and 85.7%, respectively. However, tumors with a background of intestinal metaplasia did not have a sufficient contrast-to-background ratio since complete intestinal metaplasia also expresses DPP-IV. Immunohistochemistry measurements revealed that all fluorescent tumor lesions expressed DPP-IV. Conclusions: Fluorescence imaging with EP-HMRG could be useful for the detection of early EGJ adenocarcinoma lesions that do not have a background of intestinal metaplasia.

scholarly journals Effect of cyanopyrrolidine derivatives on the activity of prolylendopeptidase, acute exudative inflammation and visceral pain in mice

2020 ◽  
Vol 66 (1) ◽  
pp. 77-82
Author(s):  
E.A. Ivanova ◽  
N.N. Zolotov ◽  
V.F. Pozdnev ◽  
T.A. Voronina
Keyword(s):  
Acetic Acid ◽  
Visceral Pain ◽  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase Iv ◽  
Writhing Test ◽  
Tert Butyl

Cyanopyrrolidine derivatives benzyloxycarbonyl-methionyl-cyanopyrrolidine (ZMetPrdN), benzyloxycarbonylphenylalanyl- cyanopyrrolidine (ZPhePrdN), tert-butyl-hydroxycarbonyl-glycyl-cyanopyrrolidine (BocGlyPrdN), tert-butyl-hydroxycarbonyl-methionyl-cyanopyrrolidine (BocMetPrdN) are inhibitors of prolylendopeptidase (PREP; EC 3.4.21.26) with an IC50 of 2 nM to 12 nM. ZMetPrdN, ZPhePrdN and BocMetPrdN additionally inhibited dipeptidyl peptidase IV (DPP-4; EC 3.4.14.5) with an IC50 of 1100 nM to 3200 nM. All the compounds have antinociceptive properties in the acetic acid writhing test in mice. But only cyanopyrrolidine derivatives with aromatic substituents decrease exudative inflammation. The cyanopyrrolidine derivatives also increase PREP activity and compensatorily reduce DPP-4 activity in the serum of mice three hours after the induction of inflammation. Thus, cyanopyrrolidine derivatives exhibit antinociceptive and antiexudative properties in part via their effect on PREP.

Rapid detection of superficial head and neck squamous cell carcinoma by topically spraying fluorescent probe targeting dipeptidyl peptidase-IV

Head & Neck ◽  
2018 ◽  
Vol 40 (7) ◽  
pp. 1466-1475 ◽  
Author(s):  
Takeshi Mizushima ◽  
Shunsuke Ohnishi ◽  
Yuichi Shimizu ◽  
Yutaka Hatanaka ◽  
Kanako C. Hatanaka ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Fluorescent Probe ◽  
Squamous Cell ◽  
Rapid Detection ◽  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase Iv ◽  
Neck Squamous Cell Carcinoma

scholarly journals Interaction of the fluorescent probe 7-anilino-4-methylcoumarin-3-acetic acid with .ALPHA.-globulin.

1985 ◽  
Vol 33 (4) ◽  
pp. 1522-1527 ◽  
Author(s):  
AKIRA TAKADATE ◽  
YOSHIKO OHKUBO ◽  
MITSURU IRIKURA ◽  
SHUJIRO GOYA ◽  
MASAKI OTAGIRI ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Fluorescent Probe

scholarly journals Alteration of dipeptidyl peptidase IV activity in rodents with exudative inflammation

2018 ◽  
pp. 51-57
Author(s):  
Е.А. Иванова ◽  
Н.Н. Золотов ◽  
В.Ф. Позднев ◽  
Т.А. Воронина
Keyword(s):  
Acetic Acid ◽  
Blood Serum ◽  
Immune Cells ◽  
Inflammatory Process ◽  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase Iv ◽  
Paw Edema ◽  
Inflammation Mediators ◽  
Severe Inflammation

Известно, что участие дипептидилпептидазы IV (ЕС 3.4.14.5, CD26, ДПП-4) в воспалительном процессе обусловлено ее влиянием на хемотаксис, пролиферацию и накопление иммунных клеток в тканях и ферментативным воздействием на ряд медиаторов воспаления. Целью данной работы являлось изучение изменения активности растворимой формы ДПП-4 при экссудативном воспалении у грызунов и оценка влияния ингибиторов ДПП-4 на его выраженность. Методы. Активность ДПП-4 в перитонеальном выпоте у крыс с моделью уксусного перитонита и мышей с моделью гликоген-индуцированного перитонита, а также в сыворотке крови крыс с моделью уксусного перитонита и с моделью вызванного каррагенаном отека лапы оценивали спектрофлуориметрически. Результаты. Установлено, что при экспериментальных перитонитах в экссудате мышей и крыс значимо увеличивается активность ДПП-4. Кроме того, при уксусном перитоните активность ДПП-4 достоверно возрастает и в сыворотке крови. У крыс с отеком лапы достоверного увеличения активности ДПП-4 в сыворотке крови не выявлено, что может быть обусловлено меньшей тяжестью воспалительного процесса. Введение мышам ингибиторов ДПП-4, отличающихся параметрами ингибирования фермента - ситаглиптина (IC = 25,0 ± 9,0 нмоль/л) и AlaPrdN (2-S-аланина цианопирролидин, IC = 2,0 ± 0,3 нмоль/л), не привело к достоверному изменению выраженности экссудации при уксусном перитоните у мышей. Заключение. Полученные данные позволяют полагать, что повышение активности ДПП-4 при остром экссудативном воспалении является следствием начавшегося воспалительного процесса. The role of dipeptidyl peptidase IV (ЕС 3.4.14.5., CD26, DPPIV) in inflammation is based on its effects on chemotaxis, proliferation, and accumulation of immune cells in tissues, and proteolysis of some inflammation mediators. The goal of this study was to evaluate the activity of soluble DPPIV in rodents with exudative inflammation and effects of DPPIV inhibitors on exudation. Methods. DPPIV activity was measured using the fluorometric assay in peritoneal exudate from rats with acetic acid-induced peritonitis and mice with glycogen-induced peritonitis as well as in serum of rats with acetic-induced peritonitis and carrageenan-induced paw edema. Results. The DPPIV activity was significantly increased in the exudate from mice and rats with experimental peritonitis. Furthermore, the DPPIV activity was significantly higher in blood serum of rats with acetic acid-induced peritonitis. Absence of a significant increase in the serum DPPIV activity in rats with paw edema could be due to less severe inflammation. DPPIV inhibitors with different ICs, sitagliptin (IC = 25.0 ± 9.0 nmol/l) and AlaPrdN (2-S-alanine cyanopyrrolidine, IC = 2.0 ± 0.3 nmol/l), did not significantly change the exudation intensity in mice with acetic acid-induced peritonitis. Conclusion. The increased DPPIV activity in acute exudative inflammation results from inflammatory process onset.

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